LOCUS       sequence-95397-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: SH3BGR_pCSdest
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    37..61
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(452..476)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(530..548)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(531..548)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tacgactcac tatagttcta gaggctcgag aggatcacca ctttgtacaa gaaagctggg
       61 ttctaggaat cttcgtcttc tccaggctct tctccttctg cagtttcttc ctcctcctcg
      121 gcttcccctt ccgcaccctc catggctatt tcttccgtct cttctgtggc tgtctctcct
      181 tcttcttcat tcttctcctg ggcttcaggg aggttgccca catcttcact gccctctttt
      241 tgtgcctcag tttctccacc ttcttcagcc ttctctgatc cctttgagtc tggaggagga
      301 gccaaaccaa ggaaggaata aataatattc tcttcttttg cagagaagaa agagtcaaaa
      361 tccccacagt actgctcctc attgaagatc tggggaggca aaggaatccc attttgaggt
      421 tttttctctc caggaacatt ctctctcatg gtgcctgctt ttttgtacaa acttgtgatc
      481 cttgaattcg aatcgatggg atcctgcaaa aagaacaagt agcttgtatt ctatagtgtc
      541 acctaaatca agcttgctcc gacgtcccca ggcagaatgg cggttcccta aacgagcatt
      601 gcttatatag acctcccatt aggcacgcct accgcccatt tacgtcaatg gaacgcccat
      661 ttgcgtcatt gcccctcccc attgacgtca atggggatgt acttggcagc catcgcgggc
      721 catttaccgc cattgacgtc aatgggagta ctgccaatgt accctggcgt acttccaata
      781 gtaatgtact tgccaagtta ctattaatag atattgatgt actgccaagt
//
