LOCUS       sequence-95402-e         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: UBE2G2_pCSdest
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    37..61
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(479..503)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(557..575)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(558..575)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tacgactcac tatagttcta gaggctcgag aggatcacca ctttgtacaa gaaagctggg
       61 ttctacagtc ccagagactt ctggacgatc tgcttggcaa tcttatagaa ctgctcccgg
      121 tcatcgcgcc acattttgga cgcatccacg ttagctccac tttcgtcatt gggctctgcc
      181 agcatgctca ccaccgacag caggatcttc tccacactct gcacaggact ccaccgctcc
      241 gcgctgctct cgtagcccat ggggtcatcg cctggcgcgt ggaggatgga aatgcagact
      301 ctcccatcag ggtagatgtt gggatgaaac atctcacagg taaatctcat ctttggggga
      361 cttaacgggt aatcaagtgg gaaactcagg atggcaggaa aaacaccaaa ctcaaagcag
      421 gtgtcttctg ggcccatgat caatgcctcc cattcaaaaa agttctcttc attcatggtg
      481 cctgcttttt tgtacaaact tgtgatcctt gaattcgaat cgatgggatc ctgcaaaaag
      541 aacaagtagc ttgtattcta tagtgtcacc taaatcaagc ttgctccgac gtccccaggc
      601 agaatggcgg ttccctaaac gagcattgct tatatagacc tcccattagg cacgcctacc
      661 gcccatttac gtcaatggaa cgcccatttg cgtcattgcc cctccccatt gacgtcaatg
      721 gggatgtact tggcagccat cgcgggccat ttaccgccat tgacgtcaat gggagtactg
      781 ccaatgtacc ctggcgtact tccaatagta atgtacttgc caagttacta ttaatagata
      841 ttgatgtact gccaagt
//
