LOCUS       sequence-95405-e         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Self-inactivating retrovirus vector containing the GFP gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Fujita T, Fujii H
  TITLE     Identification of Proteins Associated with an IFNgamma-Responsive 
            Promoter by a Retroviral Expression System for enChIP Using CRISPR.
  JOURNAL   PLoS One. 2014 Jul 22;9(7):e103084. doi: 
            10.1371/journal.pone.0103084. eCollection 2014.
  PUBMED    25051498
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-07-22"; volume: "9(7):e103084. doi"; pages: " 
            10.1371/journal.pone.0103084. eCollection 2014"
COMMENT     Sequence Label: pSIR-GFP
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             77..493
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     423..445
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     461..477
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
ORIGIN
        1 ttgtctgctg cagcatcgtt ctgtgttgtc tctgtctgac tgtgtttctg tatttgtctg
       61 aaaatatggg cccgcgggcc agactgttac cactccctta agtttgacct taggtcactg
      121 gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga gacgttgggt
      181 taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag acggcacctt
      241 taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc cgcatggaca
      301 cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc cccctccctg
      361 ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg ccccgtctct
      421 cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag ccctcactcc
      481 ttctctaggc gccaaaccta aacctcaagt tctttctgac agtggggggc cgctcatcga
      541 cctacttaca gaagaccccc cgccttatag ggacccaaga ccaccccctt ccgacaggga
      601 cggaaatggt ggagaagcga cccctgcggg agaggcaccg gacccctccc caatggcatc
      661 tcgcctacgt gggagacggg agccccctgt ggccgactcc actacctcgc aggcattccc
      721 cctccgcgca ggaggaaacg gacagcttca atactggccg ttctcctctt ctgaccttta
      781 caactggaaa aataataacc cttctttttc tgaagatcca ggtaaactga cagctctgat
      841 cgagtctgtc ctcatcaccc atcagcccac tg
//
