LOCUS       sequence-95410-e         755 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: SUMO3_pCSdest
FEATURES             Location/Qualifiers
     source          1..755
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    37..61
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(99..374)
                     /codon_start=1
                     /product="human small ubiquitin-related modifier 3"
                     /label=SUMO3
                     /translation="MSEEKPKEGVKTENDHINLKVAGQDGSVVQFKIKRHTPLSKLMKA
                     YCERQGLSMRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQQTGG"
     protein_bind    complement(377..401)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(455..473)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(456..473)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tacgactcac tatagttcta gaggctcgag aggatcacca ctttgtacaa gaaagctggg
       61 ttctagaaac tgtgccctgc caggctgctc tccggcacac ctcccgtctg ctgctggaac
      121 acgtcgatgg tgtcctcgtc ctccatctcc agctgtgctg gagtgtcagt ttcattgatt
      181 ggctgcccgt cgaacctgaa tctgatctgc ctcattgaca agccctgcct ctcgcagtag
      241 gccttcatca gcttgctcag cggcgtgtgc ctcttgatct tgaactgcac cacggagccg
      301 tcctgcccgg ccaccttcag gttgatgtgg tcattctctg tcttcacacc ctccttgggc
      361 ttctcctcgg acatggtgcc tgcttttttg tacaaacttg tgatccttga attcgaatcg
      421 atgggatcct gcaaaaagaa caagtagctt gtattctata gtgtcaccta aatcaagctt
      481 gctccgacgt ccccaggcag aatggcggtt ccctaaacga gcattgctta tatagacctc
      541 ccattaggca cgcctaccgc ccatttacgt caatggaacg cccatttgcg tcattgcccc
      601 tccccattga cgtcaatggg gatgtacttg gcagccatcg cgggccattt accgccattg
      661 acgtcaatgg gagtactgcc aatgtaccct ggcgtacttc caatagtaat gtacttgcca
      721 agttactatt aatagatatt gatgtactgc caagt
//
