LOCUS       sequence-95416-e         691 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Self-inactivating retrovirus vector containing the GFP gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Fujita T, Fujii H
  TITLE     Identification of Proteins Associated with an IFNgamma-Responsive 
            Promoter by a Retroviral Expression System for enChIP Using CRISPR.
  JOURNAL   PLoS One. 2014 Jul 22;9(7):e103084. doi: 
            10.1371/journal.pone.0103084. eCollection 2014.
  PUBMED    25051498
REFERENCE   2  (bases 1 to 691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-07-22"; volume: "9(7):e103084. doi"; pages: " 
            10.1371/journal.pone.0103084. eCollection 2014"
COMMENT     Sequence Label: pSIR-GFP
FEATURES             Location/Qualifiers
     source          1..691
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     203..224
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      266..275
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg gctgaagcac
       61 tgcacgccgt aggtcagggt ggtcacgagg gtgggccagg gcacgggcag cttgccggtg
      121 gtgcagatga acttcagggt cagcttgccg taggtggcat cgccctcgcc ctcgccggac
      181 acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg caccaccccg
      241 gtgaacagct cctcgccctt gctcaccatg gtggcgatgg atcagatcga tcttgatccc
      301 ctgcgccatc agatccttgg cggcaagaaa gccatccagt ttactttgca gggcttccca
      361 accttaccag agggcgcccc agctggcaat tccggttcgc ttgctgtctc gacgcgcttt
      421 gtgaggcctc tggcctgagg cagcgccttt atacgacagt tggcggaccg aactgagaac
      481 ctgaaagaag tcggcgggaa gtcccgcccc ggtgggggag gggaaatcta aagggccaaa
      541 ccgaaatagg ggggaaaaaa aagcgagctt cttgtttccg tgttctgaat tttgtaatgt
      601 gcaaagtatt ctgttaccac gttatgaggc tttaaaaaat tgcttttgaa cgcagaagat
      661 atacatcaat actgtgggga atacaaaaaa a
//
