LOCUS       sequence-95419-e         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: PTTG1IP_pCSdest
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    37..61
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(608..632)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(686..704)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(687..704)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tacgactcac tatagttcta gaggctcgag aggatcacca ctttgtacaa gaaagctggg
       61 ttctagttgt tttcaaatct agcatacggg ttttcttctt taaacaggcc atattttttt
      121 ctgatttcat catgtcttgt cttcatctct gctctccgtt cctcctgccg tatccgcctc
      181 tcctcccgct cacgcatggc cttctcctca ctcctgtccg gcttccggct cctcttcctc
      241 ctgcagcagc agcagcagca gatggcaatg cccaggagga gggttccccc gactaccgac
      301 atggtgatga tcagcgcctc aaagttcacc caacaaactc cccagcgtgc agagctcaat
      361 ttacaaaggg aagccggtgg caagacgctt gtaactgggt agtccagaca agccttgtta
      421 gtgttgcacc aaagacagga gacgttcttc aggcactctt cacaggtttt gtttgtgttc
      481 tgagaacaag cagctccggg aggctcctgc gcggcggcca ccgggatgag cagcaggagc
      541 agcgcggcgc caccgaggcg caacctccag tacggcgtcg gcccgcgggc cactccgggc
      601 gccatggtgc ctgctttttt gtacaaactt gtgatccttg aattcgaatc gatgggatcc
      661 tgcaaaaaga acaagtagct tgtattctat agtgtcacct aaatcaagct tgctccgacg
      721 tccccaggca gaatggcggt tccctaaacg agcattgctt atatagacct cccattaggc
      781 acgcctaccg
//
