LOCUS       sequence-9542-ex       10912 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of ChR2 (C128T) Step Function Opsin (SFO) 
            fused to EYFP, for bi-stable optogenetic excitation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10912)
  AUTHORS   Berndt A, Yizhar O, Gunaydin LA, Hegemann P, Deisseroth K
  TITLE     Bi-stable neural state switches.
  JOURNAL   Nat Neurosci. 2009 Feb . 12(2):229-34.
  PUBMED    19079251
REFERENCE   2  (bases 1 to 10912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2009 Feb . 12(2):229-34."
COMMENT     Sequence Label: pLenti-CaMKIIa-hChR2(C128T)-EYFP-WPRE
FEATURES             Location/Qualifiers
     source          1..10912
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2602..3890
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     regulatory      3912..3921
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3918..4844
                     /codon_start=1
                     /product="H134R mutant of an N-terminal fragment of 
                     channelrhodopsin-2 from the alga Chlamydomonas reinhardtii 
                     (Nagel et al., 2005)"
                     /label=ChR2(H134R)
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTTPVILIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     regulatory      4854..4863
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4860..4862,4863..4865,4866..5579)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4905..4926)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5166..5185)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5513..5534
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5604..6192
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5657..5677)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6075..6086)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6195..6211)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6196..6212)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6717..6897
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6923..6940)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6929..7153
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7199..7627
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7286..7305)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7496..7517
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7636..7656)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7641..7970
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7821..7956
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7883..7902
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8018..8065
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8084..8458
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8588..8709
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8625..8644)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8679..8698
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8758..8774)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8758..8774)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8771..8793)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8782..8798
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8806..8812,8813..8830,8831..8836))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8851..8872
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8989..9006)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9160..9748)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9240..9259)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9919..10710,10711..10779))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10542..10561
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10780..10884)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta acattatggc cttaggtcac ttcatctcca tggggttctt
     2641 cttctgattt tctagaaaat gagatggggg tgcagagagc ttcctcagtg acctgcccag
     2701 ggtcacatca gaaatgtcag agctagaact tgaactcaga ttactaatct taaattccat
     2761 gccttggggg catgcaagta cgatatacag aaggagtgaa ctcattaggg cagatgacca
     2821 atgagtttag gaaagaagag tccagggcag ggtacatcta caccacccgc ccagccctgg
     2881 gtgagtccag ccacgttcac ctcattatag ttgcctctct ccagtcctac cttgacggga
     2941 agcacaagca gaaactggga caggagcccc aggagaccaa atcttcatgg tccctctggg
     3001 aggatgggtg gggagagctg tggcagaggc ctcaggaggg gccctgctgc tcagtggtga
     3061 cagatagggg tgagaaagca gacagagtca ttccgtcagc attctgggtc tgtttggtac
     3121 ttcttctcac gctaaggtgg cggtgtgata tgcacaatgg ctaaaaagca gggagagctg
     3181 gaaagaaaca aggacagaga cagaggccaa gtcaaccaga ccaattccca gaggaagcaa
     3241 agaaaccatt acagagacta caagggggaa gggaaggaga gatgaattag cttcccctgt
     3301 aaaccttaga acccagctgt tgccagggca acggggcaat acctgtctct tcagaggaga
     3361 tgaagttgcc agggtaacta catcctgtct ttctcaagga ccatcccaga atgtggcacc
     3421 cactagccgt taccatagca actgcctctt tgccccactt aatcccatcc cgtctgttaa
     3481 aagggcccta tagttggagg tgggggaggt aggaagagcg atgatcactt gtggactaag
     3541 tttgttcgca tccccttctc caaccccctc agtacatcac cctgggggaa cagggtccac
     3601 ttgctcctgg gcccacacag tcctgcagta ttgtgtatat aaggccaggg caaagaggag
     3661 caggttttaa agtgaaaggc aggcaggtgt tggggaggca gttaccgggg caacgggaac
     3721 agggcgtttc ggaggtggtt gccatgggga cctggatgct gacgaaggct cgcgaggctg
     3781 tgagcagcca cagtgccctg ctcagaagcc ccaagctcgt cagtcaagcc ggttctccgt
     3841 ttgcactcag gagcacgggc aggcgagtgg cccctagttc tgggggcagc gggggatccc
     3901 cgggtaccgg tgccaccatg gactatggcg gcgctttgtc tgccgtcgga cgcgaacttt
     3961 tgttcgttac taatcctgtg gtggtgaacg ggtccgtcct ggtccctgag gatcaatgtt
     4021 actgtgccgg atggattgaa tctcgcggca cgaacggcgc tcagaccgcg tcaaatgtcc
     4081 tgcagtggct tgcagcagga ttcagcattt tgctgctgat gttctatgcc taccaaacct
     4141 ggaaatctac atgcggctgg gaggagatct atgtgtgcgc cattgaaatg gttaaggtga
     4201 ttctcgagtt cttttttgag tttaagaatc cctctatgct ctaccttgcc acaggacacc
     4261 gggtgcagtg gctgcgctat gcagagtggc tgctcactac tcctgtcatc cttatccacc
     4321 tgagcaacct caccggcctg agcaacgact acagcaggag aaccatggga ctccttgtct
     4381 cagacatcgg gactatcgtg tggggggcta ccagcgccat ggcaaccggc tatgttaaag
     4441 tcatcttctt ttgtcttgga ttgtgctatg gcgcgaacac attttttcac gccgccaaag
     4501 catatatcga gggttatcat actgtgccaa agggtcggtg ccgccaggtc gtgaccggca
     4561 tggcatggct gtttttcgtg agctggggta tgttcccaat tctcttcatt ttggggcccg
     4621 aaggttttgg cgtcctgagc gtctatggct ccaccgtagg tcacacgatt attgatctga
     4681 tgagtaaaaa ttgttggggg ttgttgggac actacctgcg cgtcctgatc cacgagcaca
     4741 tattgattca cggagatatc cgcaaaacca ccaaactgaa catcggcgga acggagatcg
     4801 aggtcgagac tctcgtcgaa gacgaagccg aggccggagc cgtgccagcg gccgccacca
     4861 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     4921 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     4981 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     5041 tcgtgaccac cttcggctac ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc
     5101 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     5161 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     5221 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     5281 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     5341 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     5401 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     5461 acctgagcta ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     5521 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaag
     5581 aattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga
     5641 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     5701 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     5761 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     5821 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     5881 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     5941 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     6001 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     6061 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     6121 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     6181 ccgcctcccc gcatcgatac cgtcgacctc gagacctaga aaaacatgga gcaatcacaa
     6241 gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg
     6301 aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg
     6361 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     6421 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc
     6481 agaactacac accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc
     6541 tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt
     6601 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt
     6661 ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt
     6721 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     6781 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     6841 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg
     6901 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     6961 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     7021 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     7081 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     7141 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     7201 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     7261 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     7321 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     7381 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     7441 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     7501 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     7561 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     7621 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     7681 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     7741 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     7801 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     7861 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     7921 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     7981 gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata
     8041 gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag
     8101 tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg
     8161 gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt
     8221 gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt
     8281 gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt
     8341 ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt
     8401 cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca
     8461 cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     8521 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     8581 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     8641 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     8701 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
     8761 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     8821 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     8881 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     8941 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     9001 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     9061 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     9121 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     9181 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     9241 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     9301 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     9361 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     9421 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     9481 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     9541 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     9601 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     9661 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     9721 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     9781 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     9841 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     9901 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     9961 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
    10021 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
    10081 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
    10141 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
    10201 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
    10261 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
    10321 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
    10381 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
    10441 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
    10501 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    10561 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    10621 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    10681 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    10741 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    10801 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    10861 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg ac
//
