LOCUS       sequence-95422-e         791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP-Tagged and mutated CERT PH domain in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Roy A, Levine TP
  TITLE     Multiple pools of phosphatidylinositol 4-phosphate detected using 
            the pleckstrin homology domain of Osh2p.
  JOURNAL   J Biol Chem. 2004 Oct 22;279(43):44683-9. Epub 2004 Jul 22.
  PUBMED    15271978
REFERENCE   2  (bases 1 to 791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2004-10-22"; volume: "279(43)"; pages: "44683-9. Epub
            2004 Jul 22"
COMMENT     Sequence Label: pRS406-PHO5-GFP-CERT PH domain EE mutation
FEATURES             Location/Qualifiers
     source          1..791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(194..409)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(433..452)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     552..574
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(612..630)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gatatccaca tggtgttttt agtaaacaaa ttttgggacc taatgcttca actaactcca
       61 gtaattcctt ggtggtacga acatccaatg aagcacacaa gtttgtttgc ttttcgtgca
      121 tgatattaaa tagcttggca gcaacaggac taggatgagt agcagcacgt tccttatatg
      181 tagctttcga catgatttat cttcgtttcc tgcaggtttt tgttctgtgc agttgggtta
      241 agaatactgg gcaatttcat gtttcttcaa cactacatat gcgtatatat accaatctaa
      301 gtctgtgctc cttccttcgt tcttccttct gttcggagat taccgaatca aaaaaatttc
      361 aaggaaaccg aaatcaaaaa aaagaataaa aaaaaaatga tgaattgaaa aggtggtatg
      421 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
      481 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
      541 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
      601 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
      661 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
      721 tttctaaata cattcaaata tgtatccgct catgagacaa ttaaccctga taaatgcttt
      781 caataatatt g
//
