LOCUS       sequence-95423-e         791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP-Tagged Scs2 in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Loewen CJ, Roy A, Levine TP
  TITLE     A conserved ER targeting motif in three families of lipid binding 
            proteins and in Opi1p binds VAP.
  JOURNAL   EMBO J. 2003 May 1;22(9):2025-35.
  PUBMED    12727870
REFERENCE   2  (bases 1 to 791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2003-05-1"; volume: "22(9)"; pages: "2025-35"
COMMENT     Sequence Label: pRS406-PHO5-GFP-Scs2
FEATURES             Location/Qualifiers
     source          1..791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(196..411)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(435..454)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     554..576
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(614..632)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gatatccaca tggtggtttt tagtaaacaa attttgggac ctaatgcttc aactaactcc
       61 agtaattcct tggtggtacg aacatccaat gaagcacaca agctttgttt gcttttcgtg
      121 catgatatta aatagcttgg cagcaacagg actaggatga gtagcagcac gttccttata
      181 tgtagctttc gacatgattt atcttcgttt cctgcaggtt tttgttctgt gcagttgggt
      241 taagaatact gggcaatttc atgtttcttc aacactacat atgcgtatat ataccaatct
      301 aagtctgtgc tccttccttc gttcttcctt ctgttcggag attaccgaat caaaaaaatt
      361 tcaaggaaac cgaaatcaaa aaaaagaata aaaaaaaaat gatgaattga aaaggtggta
      421 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
      481 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
      541 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
      601 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
      661 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
      721 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
      781 caataatatt g
//
