LOCUS       sequence-95424-e         757 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP-Tagged CERT PH domain in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Levine TP, Munro S
  TITLE     Targeting of Golgi-specific pleckstrin homology domains involves 
            both PtdIns 4-kinase-dependent and -independent components.
  JOURNAL   Curr Biol. 2002 Apr 30;12(9):695-704.
  PUBMED    12007412
REFERENCE   2  (bases 1 to 757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr 
            Biol."; date: "2002-04-30"; volume: "12(9)"; pages: "695-704"
COMMENT     Sequence Label: pRS406-PHO5-GFP-CERT PH domain
FEATURES             Location/Qualifiers
     source          1..757
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(193..408)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(432..451)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     551..573
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(611..629)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gatatccaca tgtgttttta gtaaacaaat tttgggacct aatgcttcaa ctaactccag
       61 taattccttg gtggtacgaa catccaatga agcacacaag tttgtttgct tttcgtgcat
      121 gatattaaat agcttggcag caacaggact aggatgagta gcagcacgtt ccttatatgt
      181 agctttcgac atgatttatc ttcgtttcct gcaggttttt gttctgtgca gttgggttaa
      241 gaatactggg caatttcatg tttcttcaac actacatatg cgtatatata ccaatctaag
      301 tctgtgctcc ttccttcgtt cttccttctg ttcggagatt accgaatcaa aaaaatttca
      361 aggaaaccga aatcaaaaaa aagaataaaa aaaaaatgat gaattgaaaa ggtggtatgg
      421 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
      481 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
      541 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
      601 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
      661 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
      721 ttctaaatac attcaaatat gtatccgctc atgagac
//
