LOCUS       sequence-95429-e         722 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pRS416 carrying Gateway cassette for a single insert.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 722)
  TITLE     Fluorescent X-QTL
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: p416 1-F GW
FEATURES             Location/Qualifiers
     source          1..722
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(103..227)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(260..276)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(261..277)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(301..320)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(302..320)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(330..347)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(330..346)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(339..361)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(574..593)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat
       61 aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga ctggatatgt
      121 tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata
      181 tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggtgat gggctgcagg
      241 aattcgatat caagcttatc gataccgtcg acctcgaggg ggggcccggt acccaattcg
      301 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
      361 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
      421 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
      481 gaatggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
      541 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
      601 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
      661 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
      721 gt
//
