LOCUS       sequence-95430-e         567 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pRS416 carrying Gateway cassette for a single insert.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 567)
  TITLE     Fluorescent X-QTL
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 567)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: p416 1-F GW
FEATURES             Location/Qualifiers
     source          1..567
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(85..91,92..109,110..115))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(140..264)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(272..288)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(272..288)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(325..345)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(325..343)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(364..380)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(364..380)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(377..399)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    388..404
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(412..418,419..436,437..442))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    457..478
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg
       61 acggatccta actcaaaatc cacacattat acgagccgga agcataaagt gtaaagcctg
      121 gggtgcctaa tgcggccgcc atagtgactg gatatgttgt gttttacagt attatgtagt
      181 ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg
      241 ttcagctttt ttgtacaaac ttgtgatggg ggatccacta gttctagagc ggccgccacc
      301 gcggtggagc tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc
      361 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
      421 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
      481 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
      541 aatcggccaa cgcgcgggga gaggcgg
//
