LOCUS       sequence-95432-e         324 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CEN vector with Hygromycin resistance marker for single Gateway 
            insertion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 324)
  TITLE     Fluorescent X-QTL
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 324)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: p41Hyg 1-F GW
FEATURES             Location/Qualifiers
     source          1..324
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(48..172)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(205..221)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(206..222)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(240..258)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 aatataaatg tcaggctccc ttatacacag ccagtctgca ggtcgaccat agtgactgga
       61 tatgttgtgt tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat
      121 tgatatttat atcattttac gtttctcgtt cagctttctt gtacaaagtg gtgatgggct
      181 gcaggaattc gatatcaagc ttatcgatac cgtcgacctc gagtcatgta attagttatg
      241 tcacgcttac attcacgccc tccccccaca tccgctctaa ccgaaaagga aggagttaga
      301 caacctgaag tctaggtccc tatt
//
