LOCUS       sequence-95433-e         214 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CEN vector with Hygromycin resistance marker for single Gateway 
            insertion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 214)
  TITLE     Fluorescent X-QTL
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 214)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: p41Hyg 1-F GW
FEATURES             Location/Qualifiers
     source          1..214
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(25..31,32..49,50..55))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(80..204)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 acggatccta actcaaaatc cacacattat acgagccgga agcataaagt gtaaagcctg
       61 gggtgcctaa tgcggccgcc atagtgactg gatatgttgt gttttacagt attatgtagt
      121 ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg
      181 ttcagctttt ttgtacaaac ttgtgatggg ggat
//
