LOCUS       sequence-95438-e         361 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Multicopy with clonNat resistance marker for single Gateway 
            insertion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 361)
  TITLE     Fluorescent X-QTL
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 361)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: p42Nat 1-F GW
FEATURES             Location/Qualifiers
     source          1..361
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(77..83,84..101,102..107))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(132..256)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(264..280)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(264..280)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(317..337)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(317..335)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct cgacggatcc
       61 taactcaaaa tccacacatt atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
      121 aatgcggccg ccatagtgac tggatatgtt gtgttttaca gtattatgta gtctgttttt
      181 tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcagctt
      241 ttttgtacaa acttgtgatg ggggatccac tagttctaga gcggccgcca ccgcggtgga
      301 gctccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat
      361 a
//
