LOCUS       sequence-95446-e         580 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CEN vector with Hygromycin resistance marker for triple Gateway 
            insertion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 580)
  TITLE     Fluorescent X-QTL
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: p41Hyg 3-F GW
FEATURES             Location/Qualifiers
     source          1..580
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(359..482)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(503..519)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(503..519)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(556..576)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(556..574)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ggggaatata aatgtcaggc tcccttatac acagccagtc tgcaggtcga tacagtagaa
       61 attacagaaa ctttatcacg tttagtaagt atagaggctg aaaatccaga tgaagccgaa
      121 cgacttgtaa gagaaaagta taagagttgt gaaattgttc ttgatgcaga tgattttcag
      181 gactatgaca ctagcgtata tgaataggta gatgttttta ttttgtcaca caaaaaagag
      241 gctcgcacct ctttttctta tttcttttta tgatttaata cggcattgag gacaatagcg
      301 agtaggctgg atacgacgat tccgtttgag aagaacattt ggaaggctgt cggtcgacca
      361 tagtgactgg atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta
      421 atttaatata ttgatattta tatcatttta cgtttctcgt tcaacttttc tatacaaagt
      481 tgatagcttg gcgtaatcgg gggatccact agttctagag cggccgccac cgcggtggag
      541 ctccagcttt tgttcccttt agtgagggtt aattgcgcgc
//
