LOCUS       sequence-95447-e         625 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CEN vector with Hygromycin resistance marker for triple Gateway 
            insertion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 625)
  TITLE     Fluorescent X-QTL
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 625)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: p41Hyg 3-F GW
FEATURES             Location/Qualifiers
     source          1..625
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(32..134)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    complement(280..403)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(451..467)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(452..468)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(486..504)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg
       61 ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc
      121 ttacgtgccg atcaacgtct cattttcgcc aaaagttggc ccagggcttc ccggtatcaa
      181 cagggacacc aggatttatt tattctgcga agtgatcttc cgtcacaggt atttattcgg
      241 cgcaaagtgc gtcgggtgat gctgccaact tacggccgcc atagtgactg gatatgttgt
      301 gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt
      361 atatcatttt acgtttctcg ttcaacttta ttatacatag ttgataattc actggccgtc
      421 ggggctgcag gaattcgata tcaagcttat cgataccgtc gacctcgagt catgtaatta
      481 gttatgtcac gcttacattc acgccctccc cccacatccg ctctaaccga aaaggaagga
      541 gttagacaac ctgaagtcta ggtccctatt tattttttta tagttatgtt agtattaaga
      601 acgttattta tatttcaaat ttttc
//
