LOCUS       sequence-95451-e         530 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Multicopy with clonNat resistance marker for triple Gateway 
            insertion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 530)
  TITLE     Fluorescent X-QTL
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 530)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: p42Nat 3-F GW
FEATURES             Location/Qualifiers
     source          1..530
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(33..135)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    complement(281..404)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(452..468)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(453..469)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(487..505)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc
       61 gataactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct
      121 cttacgtgcc gatcaacgtc tcattttcgc caaaagttgg cccagggctt cccggtatca
      181 acagggacac caggatttat ttattctgcg aagtgatctt ccgtcacagg tatttattcg
      241 gcgcaaagtg cgtcgggtga tgctgccaac ttacggccgc catagtgact ggatatgttg
      301 tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt
      361 tatatcattt tacgtttctc gttcaacttt attatacata gttgataatt cactggccgt
      421 cggggctgca ggaattcgat atcaagctta tcgataccgt cgacctcgag tcatgtaatt
      481 agttatgtca cgcttacatt cacgccctcc ccccacatcc gctctaaccg
//
