LOCUS       sequence-95465-e       14786 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  drives ZIF-1 protein in C. elegans intestinal cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14786)
  AUTHORS   Armenti ST, Lohmer LL, Sherwood DR, Nance J
  TITLE     Repurposing an endogenous degradation system for rapid and targeted 
            depletion of C. elegans proteins.
  JOURNAL   Development. 2014 Dec;141(23):4640-7. doi: 10.1242/dev.115048. Epub 
            2014 Nov 5.
  PUBMED    25377555
REFERENCE   2  (bases 1 to 14786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.115048"; journalName: "Development"; date: "2014-12"; 
            volume: "141"; issue: "23"; pages: "4640-7"
COMMENT     Sequence Label: pSA109
FEATURES             Location/Qualifiers
     source          1..14786
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3700..3711
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7455..7472)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7455..7471)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9179..9198)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9298..9320
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9358..9376)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9444..9548
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9549..9617,9618..10409)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9767..9786)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10580..11168
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11069..11088
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(12804..12815)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ggtcgagctg aatacacgtg cacaagtctg catgcagcat tttagatgaa ccatgtcttt
       61 tttcaaagtc taagggtgaa atcacgtcct ttctgcatat tggaggagtt ttcgtgtaaa
      121 aattttaaac cgtttttttt tctaacatag tcaaaaaacg aaagcagaat attaacatga
      181 ttgatatgtg tttttagggg cttcattgac attttcagca gaaaattatt cgtctgtgtc
      241 ggttcaagta aactgtttct tgaagttatc gtgatttttc agagtatgcc aaagaaacaa
      301 atcaaataat gttgtggttc tgaggaagaa agttatcctc tttgcccact ggctccgttt
      361 caaaaaataa aaccaagtat gaactttatt tatttgcatc agaacttgga tagaacattt
      421 ttgcatttta tcaaatggac ccaagtacaa gctataattt atttaaagaa gtttacacat
      481 cgtttctttc gattttcatt tttagtaaat agtttttcat gatctcgctc acgccaatat
      541 accgaaaaac gcaccaaatg agcattttct ttggtatata cactatccac atgctggcgt
      601 aatgaatctt catcacaaag cgaatcttca gctttcacga atttgtgtat aggaggacct
      661 gagacggata tacatcggtt tagaagtgcc aaagaagatt taatgtgcgt cacaatcatc
      721 gtaaaatgcg gcttcaagac gggccaggag ctcatttttc aattttatac tgccacctgt
      781 caacaattct cttgtgcatt cctaacaact gagaacttaa aaatggtaga cattaaaact
      841 ttaacttccc ctaaagttgc aattcctatt ataggaatcg tggttttagt ggtcgtctca
      901 acgttggtcg caatgttcta tgttaggtaa tcattacaat atgataggaa ttgccgaaag
      961 atgaattact actattacag aaacccaacg gtcactgata atatttttgg caatgcaaat
     1021 acggatgaga aagacgcatc aggggactct ccgctggatc atgctacgca atcttcggta
     1081 ggtaatatac acattgtccg caacaaaagt ttgaattgca ggtgggatct tctccttccc
     1141 atgtgctgca aaccacaact tccggcagcc acggaggtat gtgataagtt gaatgagtca
     1201 tcatgacgtt tgaaaaatta caacgaattg attaatcgac aaatttcagc agattcaacc
     1261 atttcgtcaa caacatcttt ggcatcacct gtcactttac caatgactcc atcttcagat
     1321 tcttctactc caatgcacgc catcgaaccc gttcagcatc tctaatttcg aaatgtatga
     1381 actccaattc ttgataacca attcctgact ctgtaactat tattattata atcctattac
     1441 ccaccttttt tcccattctg ctcttagttc tcccccaacc cgtacatttt ctcaactaat
     1501 aaagacaatg agaaagtgag agagaataag acgaactgaa aagaatgtgt aaacagttta
     1561 ttgcctctcg attgtaccat ctagtgtctt gtcgtgtgca taactactga taacagatcg
     1621 aatgtgaaag acccaataaa tgcttggaaa gtatcaaata tgtttagaaa tagtatttag
     1681 agacttatgt gggacgaaaa aacaggagca ttaagaatac agttgcaaac attcatcaag
     1741 ttataatcta ataatataaa ttcaaaaatg ttaatgataa aaaatgatga tcaaagtagg
     1801 aaattagtgg cgagaaaaca ataaatcatc agaaaatgac tgatcttgag tagagtgctt
     1861 tttttgtaga tttctgattc taaaactgaa aaaaaaacta aaaatgctcg atgagtcata
     1921 ctaattgaaa aataatcaga ttgaaaacgt taaaaatgta cttgctaaca tacttccatg
     1981 gttatttttg aacacaaata aagttatata catctttacc ggcaccagaa gaaatcaccg
     2041 ctaaccaatg ttcagttttt acctgaatat ccctgaaagt acaaaaaaat caatgttcaa
     2101 tcaagttttt gcatgccttc gtgtgatggc tcgcaccaag tattctgaca gttataaaat
     2161 gtttcctgtg atcatgaatt tgaaaaaaaa agaactgatg ggaaaatgtg atattcataa
     2221 atgaaatgtt taataaacaa aaaagaaccg tcatcacaaa tctactctga aacgttcaca
     2281 gttcctaatt gaccaattca gaattttttg tactgactta ataaatataa atttacagtt
     2341 ttaagaaaca taaattgaat tatataaata aaaaaaaaaa aactaaaatg aagacgtgat
     2401 ggaatgtttc acgtattaaa aaagctctcg gaccgtttga catccagaaa ttatgtgaaa
     2461 gtgattttct aaccctttga cgtcaaaaaa gtttttcttt tttgaaagat acgctgttta
     2521 gaaatactta tacagctgat tttagaatct ccaaaaaacc ttcaattact taacaaactt
     2581 tctaaataaa gaattttgat tttgattttc tttgaacttc aaaccccccg ttaaaaaacc
     2641 aaattaaaaa aaagatacca gattaatttt aaagtgaaaa atataaattt attcccgact
     2701 gttaaaaacg gtttataaac ggagtcacaa cactcatata caactatcga caccaaataa
     2761 ataacttcga aagctaattg tgtcatttcg gatatagaga gtagtgtgag cagatgtgtt
     2821 tttgcagaac taaattgatg acactattat ggaatataat gaccaaatgt taaatgtgtt
     2881 aaggtttgat atcaaaacct gtattttctt tttatacaga attgataatg ttatcttcaa
     2941 ttgatttcta cttctgagct acggcgatac gaggacgcat tctcaacgat aatgttgcca
     3001 ttttgtcctg ataatttttt tactgattgt ttcagaacac ccatagtttt tctctattaa
     3061 acgttcatcc ttgacttccc cgagtttgct ggctgaatag gaaatttgaa gacaaaaagg
     3121 aaagaatcgg ctcaaacgtc atgcaactga taaggcgacc gtacttcatt tcaaaagaag
     3181 ctcacttact gagcgcaaac attgagaaat gagagcaaaa gaagtggttt tacaacatgc
     3241 taatgttttt agaccttgta cccaataata ttactgtagt atacagttcg gagagcatat
     3301 ggttgaaatc ttgaaatacc aatttatcac tagtttgatt gtgttatcga tgtataaaga
     3361 tatattttat catttttgat tattatctga tattgtgggg tgtgaagtaa tattatgtgc
     3421 gtgtgtggct gattatcgaa aaaaactgaa aattatcaat ttttctacag gttatctttt
     3481 tttgttttat ttttcattat tgtattcttc atactcctta tcctgcccga atcacaaaag
     3541 ttccagacaa cttgaaggtc gcatcacgtt ttgtttataa gaatgttgaa ctggtcagct
     3601 ttggagacag aactgtcctc caaatgttga atatgtttcc gtaaaacaca ctgtgtaaca
     3661 aaaagcgtac cctttgatcc ggtattcttg aaaaaaaaca tagaagggcg tacttttcat
     3721 atctcacaaa tatgcgtttt ttattttata ataatatcat ttattgataa ttatatgtat
     3781 ttgtatgttt tcttgtcttt aaaattatgg tgtgacatca cagtttaaaa attatatgaa
     3841 aactaatgag tgaaaagtta aaaatgtata gtttgccgta ttttctccat tagtattgta
     3901 ttcaaaactt atttccaatt ggtgagcact ataaactttg tagggtaatt gaggttcaat
     3961 actaattttt gaatatctag cttttgacaa attattcaag ttttgacaat gatgccttat
     4021 caaattaaaa aaaattacca taaattcttg aaagcttttg attttgtttc actctgtgaa
     4081 ggatagtaat ttatcaacat tttaactaga tacatctgtt ctggcaataa attaaagcaa
     4141 gactattact atgtgcagta ttaatagctg cattgcaact tgtttgaaag tttcagtgaa
     4201 aacgcaaaaa ctcaatttaa ttttcaagtt tgtttgcaaa attggatata tgtatccatc
     4261 aaaagtctga tcgcctgttt tatctactta ttgtctcctc agttgatttt tttgtagtta
     4321 ttataaacgc tactttgaaa ccaatatttt cttcctcatt ccactttttt caattaccaa
     4381 ctaccgtaca tgcaatgatg ggcggacccg agtcccatgt ttggctggaa gtgggtggtt
     4441 gtctgcgtat atgagcgaca gaggtcgggg ctgaaactga taagaatagt cgacactaac
     4501 gccataatcg ctagccagcc atcatgcaca ccgagctcgg tgtgcacacc atctttcttt
     4561 tcaaaccaat acgctttgtg ccttcattga caattttctt tttgataaaa tcagcctatc
     4621 tatacttccc aatcattttt agtcttatcg ttgaacagct atcgaggtgc cactgttttc
     4681 actgatatct tctaagttac tatggcatta acatctttgt gttcttattt tatgggttat
     4741 tttaattaat ttttgcagtt aatttttgga atgagcaaga aaatgttaat tgtaatatct
     4801 tcgtctgaaa attgtcttca aatagtttta attttaaagg cagtatttaa gaaaatacac
     4861 ttctcgaagc atttttgaat ttttgaattt ttaaactgct tgatgtttta ggtgccactg
     4921 ttttcattga taagttttga tgtataaatg cttgattttc ttggcattct aataaaatag
     4981 aaactagaaa atagattata gaatgagtga gtgttccgcg agtaccagtc agttatctac
     5041 tttctgtgta aatgagatac catggaagtt ggttccacag gtaaaaatct tgaaaaccac
     5101 tcagtttcga caaaacaaaa atttcagact tgcgacgaat tcagcgaata cgagttcata
     5161 gatgaaaaat ataggtgagg atctctagat atatatctat taaattttat aaccttttca
     5221 ggcgcctttc tgacgtagaa tcgtttgtct ggaagtggtc gccgtgtgga agatatggaa
     5281 ttgatcttcc gggcggtcgt gacgtcatta accaacaact acgtgaccgt atggctgtat
     5341 tcgatctaca tacgaacaaa tggaccctat acaccatgga ccgtggtgct ttcgaaattc
     5401 cacgagtttg ctttctctac tgggcacgaa acgatataat cggcacaata acccttgtag
     5461 taagtgtccg atctaccata cctaatccaa aaaataaaat ttcagaaaga ctatgaagca
     5521 ggaccggatg atgaaattcc attgaaattc aagcaatcat tcttctacgt atctcacgaa
     5581 acacagacat tagtgtactc tggttcatgc aagtaccgta cgaataaaac aatcgtagca
     5641 tcttattggt accatatttt cgaaaatcga cgtggcgaac cgttcattga tcacaataac
     5701 tacctgtggt tggtattctc aaacggagtg gattctctga ttcttcttcc atttattcca
     5761 acttcaacgt gtttcaaagc gaaatgtgta tcatttgata tgaacgatat cgtcagtcaa
     5821 ttcactggta ttcgcgtatt tctcgatcca tttgtatcat ctcactacgt gccatgggtc
     5881 tacagaaatg acgttaattt tttcattagg attgattcgc attcatttat cgaggagcaa
     5941 aaccgggact atggtctaga tccgcaaatt gataacccgg agacgaaacc gcacagagga
     6001 gtatttcaac ttcgtgtgaa tttgaaagat gtcatcgaaa agggcgaaaa agatgcgatt
     6061 catgcagtta atcctgaaac aatagagaca agattcaatc gaggtgacat tgaactatgg
     6121 cgaaaaatca gcgatgagct ccgtcaagaa catcagagca tagcacagca tggcaaaatg
     6181 gttgtagtac aacgatggtc acactttact gttgatatgg ggctcagcaa gaaaatgaga
     6241 accgtaggat atgaaaatat tatttgttga tagtttatta atttaatttc agcgtgttgc
     6301 cgatttggac gcccgtgatt actgttgcag ccataagcca cgtaattcgt cattctgctg
     6361 tattgacttg gacagcgagc gaatcaaggc tgtggatgct ctcattcttg ggtacaaatt
     6421 aatctatttt tttcctaagt attttttttt cagaactgac gtggttcttc cccatcctag
     6481 tggctctgta ttcctatttc gatacaagga aaattatggt aatccctcca ccaaatagac
     6541 gttttcttca tcgtaatttt tcagggatga gttattgcga gcttccattc ttccagccac
     6601 tttcattgaa gctaaaatgt attcaagttc tcaaggaatc tgtcgaaatc gacccaagtt
     6661 actatagaat caaatgctgc cttaaaatgc aagaagaact tgaaaaagaa actgccgaaa
     6721 atttgtgcac gataaatatt caacaataag tccaattact cttcaacatc cctacatgct
     6781 ctttctccct gtgctcccac cccctatttt tgttattatc aaaaaaactt cttcttaatt
     6841 tctttgtttt ttagcttctt ttaagtcacc tctaacaatg aaattgtgta gattcaaaaa
     6901 tagaattaat tcgtaataaa aagtcgaaaa aaattgtgct ccctcccccc attaataata
     6961 attctatccc aaaatctaca caatgttctg tgtacacttc ttatgttttt tacttctgat
     7021 aaattttttt gaaacatcat agaaaaaacc gcacacaaaa taccttatca tatgttacgt
     7081 ttcagtttat gaccgcaatt tttatttctt cgcacgtctg ggcctctcat gacgtcaaat
     7141 catgctcatc gtgaaaaagt tttggagtat ttttggaatt tttcaatcaa gtgaaagttt
     7201 atgaaattaa ttttcctgct tttgcttttt ggggtttccc ctattgtttg tcaagatttc
     7261 gaggacggcg tttttcttgc taaaatcaca agtattgatg agcacgatgc aagaaagatc
     7321 ggaagaaggt ttgggtttga ggctcagtgg aaggtgagta gaagttgata atttgaaagt
     7381 ggagtagtgt ctatggggtt tttgccttaa atgacagaat acattcccaa tataccaaac
     7441 ataactgttt aaacactggc cgtcgtttta cactcgagac gtacggtgcg cgcgatgcat
     7501 tcgaagatct gcccactagt gagtcgtatt ataagtgcaa gtaagatcag tgtttgtttc
     7561 gatatcagtc tgtttcgtaa cggtcttctg tataactaca aaaaagaata aaaaaccgta
     7621 tcacaactga gaaaaaagac aaaacttatt gaacagtgtt cctttcaaaa aattattttg
     7681 taaaaaatcg agtttttctc caaaataacg cacgcaatca aaaacaagaa aactaacgtg
     7741 ttctcctggg cgtggaactc cttatcaaga ttaccattat tgtctgtcag aattgatatt
     7801 tcaattattt catatctaga caaacccggg tgtcttgcat ttattagtac tgtgagttta
     7861 catacaaacg aggcttactc agataacata gagtcacctc taattagcaa aaacccattt
     7921 ctaattagct atttcgacca gttctaaagt caccgtaatt agaaatggtt tttgtagctt
     7981 ttctaattag aaatgacccc gagataagtg aaatatggca ttttctgggt tttctagtaa
     8041 gaaatgatcc cgcacgtagt gattccgcgt tacaagccaa attcaaatta aaaaagtata
     8101 ttacacaatt caagttacat tattttcagg aaacaatggc gaagagcaaa gcagagattc
     8161 aaaaggagga aagagacaac agatatgaac gaagacttct tgaaaagaga aagagacatg
     8221 aggtgagtaa ccatgataat aaataatcaa gtaagggcta tagatttata aattctggag
     8281 ttaacagttt cgaaaaaaag tagttttcat atgtgcgaag gaatatcaaa aataggggaa
     8341 tagcctaaac gagtgagaaa attaaatgtt tgttattttt tcaaaaatgt ctgcagggcc
     8401 gttatccttt caaatgcact ctatcaaaca tagatcatat ccatcatatc ttatccagaa
     8461 tttatatcat atccaagatt gagagcgttt caaggaaaat ctcaaacaac catacaattt
     8521 tgtagatcga caacaaggcc tccacttctt catcgatggc ctctgcagca cctgcaccac
     8581 caagtttgta tttttcatat taatataaag tgagccacgc atagtcgttt ttaggatttt
     8641 tgccagatcg atgtcgcgca aacagactgt ttttttttct ttgaaagccg tgcgtgacat
     8701 gggccacgga ttgtaacgcc gcgctggatg gataaaaatt tattcagttt tttagccttt
     8761 acaaaatatg gttcgactac tttctttatt tttacatttt tataggtgct cattgagctg
     8821 agatattttg ggtataatcg gaaaatcgct gattttggtc atttttctgc actaaagatt
     8881 acacaaaaag ttcagtattt agattcattg ttgaaaattg gggatgatct acagatacat
     8941 atcgccgtgt tcaaaaacac acgtgctgtg acgtcgtgac tggggaaaac cctggcgtta
     9001 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     9061 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc
     9121 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta
     9181 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
     9241 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     9301 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
     9361 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     9421 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     9481 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     9541 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     9601 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     9661 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     9721 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     9781 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     9841 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     9901 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     9961 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
    10021 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
    10081 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
    10141 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
    10201 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
    10261 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
    10321 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
    10381 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
    10441 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    10501 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    10561 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
    10621 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
    10681 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
    10741 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    10801 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    10861 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    10921 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    10981 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    11041 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    11101 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    11161 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggtcttttgc tggccttttg
    11221 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
    11281 agtgagctga taccagcgga taacaatttc acagggccct gtcggattat gggctcttct
    11341 tttatcctct actttctttc tagaaaatgc aaatgttgag tcagaaattg ctgaatggag
    11401 tacaactgca atgggtttag cttatgtttc tggaacaatt actggtggaa ttgtgattga
    11461 aaagtaaggt tatatttcat ttcaataaat gttaccacag tataacaaaa acaagttaca
    11521 ttgttcagag tatgtctgaa tgtattatta ttattgtatt tgtgcatcag tactgtagtt
    11581 gtactgaaga agggtttaaa ataatctcaa cgtttttaag acgagttagt gagaatacgg
    11641 tcgcagttag gatgtttgga catagtttta ttagataatc gtgaactaac tcactataaa
    11701 aacccgctaa ctcattgaat aagatagttt taaacgttca aaatttgaag acaaaattgt
    11761 atgaataagt ttacagagtt ggtcgacgca agcttctcct tctcttcaca tttctcaata
    11821 atttagctct aattgcattc gtattcttcg caaaaatacg tattctgatt gacccgatga
    11881 aatacggatg cctagttgca ttgatcatct acggttatac ttatgggtga ggtttttcaa
    11941 atatattttg atttaatcga ataatgaaat ttcagaactg gagttggtcc aatttcgtgg
    12001 tttatatcct ctgaactggt acctcaaaaa catcgtagta ttgctcaatc tgtggcatat
    12061 tcaatgaata ctgtaatggt ggttatcagt acatttacag tattacctct atattccgtt
    12121 attggtagtt atgctttcct tattctatat tcaattcctt catttataag tatgcttata
    12181 ttatttcgct acttaccgga aaccaaagga cgagagattc atgatattgt gaatgaactt
    12241 aaaaaaaaat gaaaaccttt gaaaacgttc atacggccga aattttactt gatcgagcca
    12301 attgaacttt tgttaagact tacagaaaag tgagagattt tgttacaaaa aagtgagaaa
    12361 aaagcgaaaa tgaacaggaa aaagaagaaa aatacacccg aagtatcata taaaaattaa
    12421 aaagattgtt tgacctggcg gaactttttt gcaaaaactc tgaatatcca tggcacatgc
    12481 ttcgtgcaaa acaggatcca aatgaatatc aacaagagat tcttgaagac gacgagccac
    12541 ttgctcagag cattgcttat ctcgaatgag acccttgtta aaatcatttt tcaaacatta
    12601 tatgacattg tcgaaatgtc ctcctgattc catgatggta gcaaactcac ttcgtctttg
    12661 agccaattta tccaagtcct tgtaaaaagt attcgaaaat tgttaacgga taaaatgttt
    12721 attataatat caaaaacaat ttgtcagttg accacttttt gatatatttg acagaaaccg
    12781 ggatgaattg gctcaaagtt agggcgccct tctattacag ggtttctgat aacaaaccgg
    12841 ttattaactc ccaacaaggg atgatttcaa ttcatcatgc tcaaattgac ccaaattaag
    12901 ttacatgaca aattcatcgc ccttttcaac tcttcttggc tcatcatctg ttattctgtt
    12961 cctattctgt ctgcacccta tacccctttg catactctct cgtcattcct ttcttggata
    13021 gtgtcttctt ctccccagct ctgctacttc tatgacttgc gcccgctgct tttccgctcg
    13081 ttcctctcct ctctgacgtc ttcttctgct tcttttctcc ttcctttttc ccttctcaat
    13141 cttcctcttt ttcccatttc tgtcaatcat tcgaagaaga agaagaagaa gaccctcatt
    13201 catttcattt ttttttctgt cggtgtgtgc tgctcggtta agagtgagct ctctattcca
    13261 cgtcttcttc tttttcttct tcgattcgaa tcaatcactc caccaaaacg cattcgtttt
    13321 tgggattcac cccgcggttc gcaataggtt tctttttcaa attattagcg ttataaatag
    13381 aaaaatggtg gagtttcaat aaaaatgata atttacaaag tgtattttga ttacatgtac
    13441 tcaaaaaggt tgaaattttt cataccagtt ttccggaaat ccatctgata tcattatcgt
    13501 atttttcttt ttaaaaatgt tttcaaaaaa aaacaaaata tagctggtta tttggcaccc
    13561 tctaattacc attttcctgt cacaccacac tcttttcctt cttccacttc tttccgtttt
    13621 cagccgcttc caaccaaacc gatatgaaag ccgagcaaca acgatcgatt ccacccggct
    13681 cggcgacctt cccgtcgcag gtgagactca gaaaactaga gaaaccgctc aactaactct
    13741 tgatatccga tttcattctt ttctcttttc tttttgttga acttttccat atttccagat
    13801 gccacggcca ccaccaagca ccgaacaagg aatcacaacg gaatcggagc ttgcgaagaa
    13861 agctcaaatc actccgaacg acgttttagc acttccggga atcactcaag gtatcttgct
    13921 ccctttggat ttcagtttaa aacataaatt taggattctt atgctcccca tctgcgaaca
    13981 tctataacat cgagttcacc aagttccaaa tccgtgatct ggacactgag caagtgcttt
    14041 tcgagatcgc caaaccggag aacgatcagg agaatgatga gtcgccacag gagtcggcaa
    14101 gatacgtgcg ttatagattt gctccaaact ttttgaaact caaaacggtc ggagcaactg
    14161 tggaattcaa agtaggagac atcccaatcc atcatttccg aatgatcgaa cgtcacttct
    14221 tcaaagatcg ccttctgaag tgttttgact ttgaattcgg attctgtatt ccgaattcac
    14281 gaaacaactg tgaacatatc tatgagttcc ctcaactctc tcaacaactc agtgagtcat
    14341 tattctaaaa agtacaattc aaaagactaa tctctttttc agtggacgac atgatcaaca
    14401 atccaaacga gactcgttct gacagcttct atttcgtcga taacaaactc gtcatgcaca
    14461 acaaagccga ctactcatat gatgcataaa tatttaatac aaaaatgttc tggataatta
    14521 ttctgtcgaa tagaaaaaaa actccaaatg tgattaaatt ccaataattc ctgtctagtt
    14581 tgttccttcc ccttcccctt ctcatgttca atgcattcct aagcttttca gttcccccct
    14641 tgtttctata ttttttcgct gtcctgtcac actcgctaaa aacactaatc acacggaaat
    14701 ctgttttcaa taaaaactcc aactttaact cattttcaat ttcaactgaa agattttttc
    14761 attagatgtc tagaactagg ccgttt
//
