LOCUS       sequence-95467-e       13777 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  drives ZIF-1 protein in C. elegans ubiquitously.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13777)
  AUTHORS   Armenti ST, Lohmer LL, Sherwood DR, Nance J
  TITLE     Repurposing an endogenous degradation system for rapid and targeted 
            depletion of C. elegans proteins.
  JOURNAL   Development. 2014 Dec;141(23):4640-7. doi: 10.1242/dev.115048. Epub 
            2014 Nov 5.
  PUBMED    25377555
REFERENCE   2  (bases 1 to 13777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.115048"; journalName: "Development"; date: "2014-12"; 
            volume: "141"; issue: "23"; pages: "4640-7"
COMMENT     Sequence Label: pSA107
FEATURES             Location/Qualifiers
     source          1..13777
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      171..759
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     913..930
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1047..1068
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1083..1088,1089..1106,1107..1113)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1121..1137
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1145..1161
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1145..1161
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1180..1200
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1182..1200
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6443..6460)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6443..6459)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8167..8186)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8286..8308
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8346..8364)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8432..8536
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8537..8605,8606..9397)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8755..8774)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9568..10156
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(11792..11803)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
       61 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
      121 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
      181 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
      241 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
      301 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
      361 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
      421 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
      481 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
      541 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
      601 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
      661 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
      721 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
      781 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
      841 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
      901 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
      961 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     1021 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     1081 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     1141 cacacaggaa acagctatga ccatgattac gccaagctcg caattaaccc tcactaaagg
     1201 gaacaaaagc tgcgattcta gaggaggtcg tcgaggcgga aaaggaaagg gaaagagtaa
     1261 tctgcaattt gcacaagttg cagaattttc gttggttcaa acggttttgt cagataatag
     1321 agcgagtttc agaaataaaa aaaatgatac aaaatttgaa agtctttcag agtgctcaaa
     1381 tcataactgg ctctcacttc agtcaaacat atcggcttgg atacaaacta cttatcattt
     1441 gcaaagctcg tggagatcct agaccgacaa ttaaatggta taaggaaggt gctgaaattc
     1501 aaccaaaagc tagcattcac gtgagtcatg tacagtaatc cgtgggttaa ctgctttact
     1561 tgcagtatta cgaaaagcca atcgaaaatg atacaatttg gagtaaatta gaagttggta
     1621 agtgaaaatt gttgtcaatc aagaatatct ttagaaccct tgcgaaaaat gatacaatat
     1681 gataagaaac ttttttgtca ctgtcagcaa attttggaat ttcgctcatt tctctacaat
     1741 ttttttgcca tattcagcgc tttttaaagt ttttcttctt aactgtgagc gaatggcaaa
     1801 ctacaataac gacagctaaa tatacgacag ttgtcatatt ttcagatccg gcaacaatgg
     1861 gcgatcaggg tgtctatgca tgtgtggcta ataacccgca cggagtaatg gcaaagaatt
     1921 ttaaagcaga atatacgtat tgaataaaga tttttcacaa atcaaataat tttctctcac
     1981 ctaatctata tcatataaac ttgcgaagga atgcgtttga aacctcctcc ttctgttgcg
     2041 tgcaggcgca cgagcgacaa aacaaacagt gtttgggagt gagagaagcg ggatgaacag
     2101 gtgtgcggtg gcggtaaatt cgagtgaagg cgggaatgtg ttcagaatga atacagaaaa
     2161 agagctgatg acgttgtaaa cgaaattcat gaacgaaaag caggcacatc tcgcgcggaa
     2221 aagggaggag acgcggcagg gaaccggatt gggggactat tgatcgaaca ataaggaaga
     2281 agagaagtac gcgccgtcgg ttatcgaaac tccaaatttt tcttctttta attgggaaaa
     2341 gtattacgaa catgtaattt catggaattc acacggttta caatgaagtt atcaagagaa
     2401 aatatgcaat tctaaaaatt tagggcagat tgaatcgtcg attggattga agtgaaagaa
     2461 gagaaataga aatttcgcga gtgagattca atcggagcat tgttagaatt ctatctagaa
     2521 atgtaagaca aaaaatacga aatagcatat tgaaatataa aaccttaaaa ttaaaaagaa
     2581 ggcaatttta aaattaattt tacgaaatac aattgagaaa aaatggaaaa cacaacggat
     2641 tcaatcgaag ttaatattct agagatggta tttataaatt gaggacaaat attctgcact
     2701 aggagaaaca agaaaaaaga aagaagtttc tggctggcaa tcgttcgcac aggaaaaata
     2761 aaaaactaaa atggccgccc gtctgttagc atggaacggg ttacgctagt tatagactaa
     2821 gcttacatac aaaatatgta tgtataatat cctcacagcg taacaaagtg agatatagtt
     2881 taaattctag atgatgactt tagaaatcct atttggtggt tttacgaagt ttacagcgtc
     2941 atttctagac tgaaactgac cagtctggga tatcatttgt ctaatcaagt actctatcta
     3001 gataactgac cgatctaata agttttcacg agttacggga cctaaatttg aatccaataa
     3061 ttcggtgacg actggcgacc ctatgttaga tgtagtgatg tctccattct ctcaatactc
     3121 tctcgtcttt ttctctgcgt atctcaccac tagcgtagtc tatgagtgcg agcggccgcg
     3181 tcgcggtcgt ctttaggctc gccgacaaac tgtccaccat cttctttttt tccttttttc
     3241 gccgtgttca acaaagctca ttcacgcttt tcgttgtttt acaggctttt attgtttttt
     3301 ctaattattg attattcatt tataagagca gaattaatca ataaacgtag gtagacgatc
     3361 gaagtatcct atcagttttt aatttgataa attttctcga aaggagcaat catgttttaa
     3421 attatttaaa aaatggtccg cttaagtttg tctgactgtt taacaattat tgaaatcatt
     3481 ttcgtgacat tttattgaac attgcttcca aattccccta acataatcct gactgagatg
     3541 ttccagtgtt tcatgtcgaa ttattcctat ttttgtacct tttcttaaat ataactagtt
     3601 tcaaatatat gcaagttgtt tggtacggaa aaccgattca aacttccgac cagttaccga
     3661 tgaatcattg ccaaaattcc tcatctaaag aattatacgc acaagttgtt ttggccattt
     3721 tgccttattt cttcatcgtt tattaaggcg tttaccgttt ttttttcttg ctctttcttc
     3781 gataattatt gctcccaatg gtaaagaaac gctcgtggca tttcgaaaga gactattgat
     3841 tttctgcctg atgtttcgca attcgttttc attctctagt tatctctcct tttaaaacgt
     3901 gtgcgtgcac atttatgtga ctcatccgta tttttcacta taaagacgta attttaatac
     3961 ttttattcat tttttttttc aggcgaaatg agtgagtgtt ccgcgagtac cagtcagtta
     4021 tctactttct gtgtaaatga gataccatgg aagttggttc cacaggtaaa aatcttgaaa
     4081 accactcagt ttcgacaaaa caaaaatttc agacttgcga cgaattcagc gaatacgagt
     4141 tcatagatga aaaatatagg tgaggatctc tagatatata tctattaaat tttataacct
     4201 tttcaggcgc ctttctgacg tagaatcgtt tgtctggaag tggtcgccgt gtggaagata
     4261 tggaattgat cttccgggcg gtcgtgacgt cattaaccaa caactacgtg accgtatggc
     4321 tgtattcgat ctacatacga acaaatggac cctatacacc atggaccgtg gtgctttcga
     4381 aattccacga gtttgctttc tctactgggc acgaaacgat ataatcggca caataaccct
     4441 tgtagtaagt gtccgatcta ccatacctaa tccaaaaaat aaaatttcag aaagactatg
     4501 aagcaggacc ggatgatgaa attccattga aattcaagca atcattcttc tacgtatctc
     4561 acgaaacaca gacattagtg tactctggtt catgcaagta ccgtacgaat aaaacaatcg
     4621 tagcatctta ttggtaccat attttcgaaa atcgacgtgg cgaaccgttc attgatcaca
     4681 ataactacct gtggttggta ttctcaaacg gagtggattc tctgattctt cttccattta
     4741 ttccaacttc aacgtgtttc aaagcgaaat gtgtatcatt tgatatgaac gatatcgtca
     4801 gtcaattcac tggtattcgc gtatttctcg atccatttgt atcatctcac tacgtgccat
     4861 gggtctacag aaatgacgtt aattttttca ttaggattga ttcgcattca tttatcgagg
     4921 agcaaaaccg ggactatggt ctagatccgc aaattgataa cccggagacg aaaccgcaca
     4981 gaggagtatt tcaacttcgt gtgaatttga aagatgtcat cgaaaagggc gaaaaagatg
     5041 cgattcatgc agttaatcct gaaacaatag agacaagatt caatcgaggt gacattgaac
     5101 tatggcgaaa aatcagcgat gagctccgtc aagaacatca gagcatagca cagcatggca
     5161 aaatggttgt agtacaacga tggtcacact ttactgttga tatggggctc agcaagaaaa
     5221 tgagaaccgt aggatatgaa aatattattt gttgatagtt tattaattta atttcagcgt
     5281 gttgccgatt tggacgcccg tgattactgt tgcagccata agccacgtaa ttcgtcattc
     5341 tgctgtattg acttggacag cgagcgaatc aaggctgtgg atgctctcat tcttgggtac
     5401 aaattaatct atttttttcc taagtatttt tttttcagaa ctgacgtggt tcttccccat
     5461 cctagtggct ctgtattcct atttcgatac aaggaaaatt atggtaatcc ctccaccaaa
     5521 tagacgtttt cttcatcgta atttttcagg gatgagttat tgcgagcttc cattcttcca
     5581 gccactttca ttgaagctaa aatgtattca agttctcaag gaatctgtcg aaatcgaccc
     5641 aagttactat agaatcaaat gctgccttaa aatgcaagaa gaacttgaaa aagaaactgc
     5701 cgaaaatttg tgcacgataa atattcaaca ataagtccaa ttactcttca acatccctac
     5761 atgctctttc tccctgtgct cccaccccct atttttgtta ttatcaaaaa acttctctta
     5821 atttctttgt tttttagctt cttttaagtc acctctaaca atgaaattgt gtagattcaa
     5881 aaatagaatt aattcgtaat aaaaagtcga aaaaaattgt gctccctccc cccattaata
     5941 ataattctat cccaaaatct acacaatgtt ctgtgtacac ttcttatgtt ttttttactt
     6001 ctgataaatt ttttttgaaa catcatagaa aaaaccgcac acaaaatacc ttatcatatg
     6061 ttacgtttca gtttatgacc gcaattttta tttcttcgca cgtctgggcc tctcatgacg
     6121 tcaaatcatg ctcatcgtga aaaagttttg gagtattttt ggaatttttc aatcaagtga
     6181 aagtttatga aattaatttt cctgcttttg ctttttgggg gtttccccta ttgtttgtca
     6241 agagtttcga ggacggcgtt tttcttgcta aaatcacaag tattgatgag cacgatgcaa
     6301 gaaagatcgg aagaaggttt gggtttgagg ctcagtggaa ggtgagtaga agttgataat
     6361 ttgaaagtgg agtagtgtct atggggtttt tgccttaaat gacagaatac attcccaata
     6421 taccaaacat aactgtttaa acactggccg tcgttttaca ctcgagacgt acggtgcgcg
     6481 cgatgcattc gaagatctgc ccactagtga gtcgtattat aagtgcaagt aagatcagtg
     6541 tttgtttcga tatcagtctg tttcgtaacg gtcttctgta taactacaaa aaagaataaa
     6601 aaaccgtatc acaactgaga aaaaagacaa aacttattga acagtgttcc tttcaaaaaa
     6661 ttattttgta aaaaatcgag tttttctcca aaataacgca cgcaatcaaa aacaagaaaa
     6721 ctaacgtgtt ctcctgggcg tggaactcct tatcaagatt accattattg tctgtcagaa
     6781 ttgatatttc aattatttca tatctagaca aacccgggtg tcttgcattt attagtactg
     6841 tgagtttaca tacaaacgag gcttactcag ataacataga gtcacctcta attagcaaaa
     6901 acccatttct aattagctat ttcgaccagt tctaaagtca ccgtaattag aaatggtttt
     6961 tgtagctttt ctaattagaa atgaccccga gataagtgaa atatggcatt ttctgggttt
     7021 tctagtaaga aatgatcccg cacgtagtga ttccgcgtta caagccaaat tcaaattaaa
     7081 aaagtatatt acacaattca agttacatta ttttcaggaa acaatggcga agagcaaagc
     7141 agagattcaa aaggaggaaa gagacaacag atatgaacga agacttcttg aaaagagaaa
     7201 gagacatgag gtgagtaacc atgataataa ataatcaagt aagggctata gatttataaa
     7261 ttctggagtt aacagtttcg aaaaaaagta gttttcatat gtgcgaagga atatcaaaaa
     7321 taggggaata gcctaaacga gtgagaaaat taaatgtttg ttattttttc aaaaatgtct
     7381 gcagggccgt tatcctttca aatgcactct atcaaacata gatcatatcc atcatatctt
     7441 atccagaatt tatatcatat ccaagattga gagcgtttca aggaaaatct caaacaacca
     7501 tacaattttg tagatcgaca acaaggcctc cacttcttca tcgatggcct ctgcagcacc
     7561 tgcaccacca agtttgtatt tttcatatta atataaagtg agccacgcat agtcgttttt
     7621 aggatttttg ccagatcgat gtcgcgcaaa cagactgttt ttttttcttt gaaagccgtg
     7681 cgtgacatgg gccacggatt gtaacgccgc gctggatgga taaaaattta ttcagttttt
     7741 tagcctttac aaaatatggt tcgactactt tctttatttt tacattttta taggtgctca
     7801 ttgagctgag atattttggg tataatcgga aaatcgctga ttttggtcat ttttctgcac
     7861 taaagattac acaaaaagtt cagtatttag attcattgtt gaaaattggg gatgatctac
     7921 agatacatat cgccgtgttc aaaaacacac gtgctgtgac gtcgtgactg gggaaaaccc
     7981 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     8041 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg
     8101 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac
     8161 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
     8221 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     8281 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
     8341 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     8401 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     8461 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     8521 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     8581 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     8641 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     8701 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     8761 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     8821 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     8881 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     8941 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     9001 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     9061 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     9121 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     9181 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     9241 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     9301 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     9361 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     9421 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     9481 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     9541 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     9601 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     9661 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     9721 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     9781 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     9841 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     9901 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     9961 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
    10021 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
    10081 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
    10141 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg tcttttgctg
    10201 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
    10261 cgcctttgag tgagctgata ccagcggata acaatttcac agggccctgt cggattatgg
    10321 gctcttcttt tatcctctac tttctttcta gaaaatgcaa atgttgagtc agaaattgct
    10381 gaatggagta caactgcaat gggtttagct tatgtttctg gaacaattac tggtggaatt
    10441 gtgattgaaa agtaaggtta tatttcattt caataaatgt taccacagta taacaaaaac
    10501 aagttacatt gttcagagta tgtctgaatg tattattatt attgtatttg tgcatcagta
    10561 ctgtagttgt actgaagaag ggtttaaaat aatctcaacg tttttaagac gagttagtga
    10621 gaatacggtc gcagttagga tgtttggaca tagttttatt agataatcgt gaactaactc
    10681 actataaaaa cccgctaact cattgaataa gatagtttta aacgttcaaa atttgaagac
    10741 aaaattgtat gaataagttt acagagttgg tcgacgcaag cttctccttc tcttcacatt
    10801 tctcaataat ttagctctaa ttgcattcgt attcttcgca aaaatacgta ttctgattga
    10861 cccgatgaaa tacggatgcc tagttgcatt gatcatctac ggttatactt atgggtgagg
    10921 tttttcaaat atattttgat ttaatcgaat aatgaaattt cagaactgga gttggtccaa
    10981 tttcgtggtt tatatcctct gaactggtac ctcaaaaaca tcgtagtatt gctcaatctg
    11041 tggcatattc aatgaatact gtaatggtgg ttatcagtac atttacagta ttacctctat
    11101 attccgttat tggtagttat gctttcctta ttctatattc aattccttca tttataagta
    11161 tgcttatatt atttcgctac ttaccggaaa ccaaaggacg agagattcat gatattgtga
    11221 atgaacttaa aaaaaaatga aaacctttga aaacgttcat acggccgaaa ttttacttga
    11281 tcgagccaat tgaacttttg ttaagactta cagaaaagtg agagattttg ttacaaaaaa
    11341 gtgagaaaaa agcgaaaatg aacaggaaaa agaagaaaaa tacacccgaa gtatcatata
    11401 aaaattaaaa agattgtttg acctggcgga acttttttgc aaaaactctg aatatccatg
    11461 gcacatgctt cgtgcaaaac aggatccaaa tgaatatcaa caagagattc ttgaagacga
    11521 cgagccactt gctcagagca ttgcttatct cgaatgagac ccttgttaaa atcatttttc
    11581 aaacattata tgacattgtc gaaatgtcct cctgattcca tgatggtagc aaactcactt
    11641 cgtctttgag ccaatttatc caagtccttg taaaaagtat tcgaaaattg ttaacggata
    11701 aaatgtttat tataatatca aaaacaattt gtcagttgac cactttttga tatatttgac
    11761 agaaaccggg atgaattggc tcaaagttag ggcgcccttc tattacaggg tttctgataa
    11821 caaaccggtt attaactccc aacaagggat gatttcaatt catcatgctc aaattgaccc
    11881 aaattaagtt acatgacaaa ttcatcgccc ttttcaactc ttcttggctc atcatctgtt
    11941 attctgttcc tattctgtct gcaccctata cccctttgca tactctctcg tcattccttt
    12001 cttggatagt gtcttcttct ccccagctct gctacttcta tgacttgcgc ccgctgcttt
    12061 tccgctcgtt cctctcctct ctgacgtctt cttctgcttc ttttctcctt cctttttccc
    12121 ttctcaatct tcctcttttt cccatttctg tcaatcattc gaagaagaag aagaagaaga
    12181 ccctcattca tttcattttt ttttctgtcg gtgtgtgctg ctcggttaag agtgagctct
    12241 ctattccacg tcttcttctt tttcttcttc gattcgaatc aatcactcca ccaaaacgca
    12301 ttcgtttttg ggattcaccc cgcggttcgc aataggtttc tttttcaaat tattagcgtt
    12361 ataaatagaa aaatggtgga gtttcaataa aaatgataat ttacaaagtg tattttgatt
    12421 acatgtactc aaaaaggttg aaatttttca taccagtttt ccggaaatcc atctgatatc
    12481 attatcgtat ttttcttttt aaaaatgttt tcaaaaaaaa acaaaatata gctggttatt
    12541 tggcaccctc taattaccat tttcctgtca caccacactc ttttccttct tccacttctt
    12601 tccgttttca gccgcttcca accaaaccga tatgaaagcc gagcaacaac gatcgattcc
    12661 acccggctcg gcgaccttcc cgtcgcaggt gagactcaga aaactagaga aaccgctcaa
    12721 ctaactcttg atatccgatt tcattctttt ctcttttctt tttgttgaac ttttccatat
    12781 ttccagatgc cacggccacc accaagcacc gaacaaggaa tcacaacgga atcggagctt
    12841 gcgaagaaag ctcaaatcac tccgaacgac gttttagcac ttccgggaat cactcaaggt
    12901 atcttgctcc ctttggattt cagtttaaaa cataaattta ggattcttat gctccccatc
    12961 tgcgaacatc tataacatcg agttcaccaa gttccaaatc cgtgatctgg acactgagca
    13021 agtgcttttc gagatcgcca aaccggagaa cgatcaggag aatgatgagt cgccacagga
    13081 gtcggcaaga tacgtgcgtt atagatttgc tccaaacttt ttgaaactca aaacggtcgg
    13141 agcaactgtg gaattcaaag taggagacat cccaatccat catttccgaa tgatcgaacg
    13201 tcacttcttc aaagatcgcc ttctgaagtg ttttgacttt gaattcggat tctgtattcc
    13261 gaattcacga aacaactgtg aacatatcta tgagttccct caactctctc aacaactcag
    13321 tgagtcatta ttctaaaaag tacaattcaa aagactaatc tctttttcag tggacgacat
    13381 gatcaacaat ccaaacgaga ctcgttctga cagcttctat ttcgtcgata acaaactcgt
    13441 catgcacaac aaagccgact actcatatga tgcataaata tttaatacaa aaatgttctg
    13501 gataattatt ctgtcgaata gaaaaaaaac tccaaatgtg attaaattcc aataattcct
    13561 gtctagtttg ttccttcccc ttccccttct catgttcaat gcattcctaa gcttttcagt
    13621 tccccccttg tttctatatt ttttcgctgt cctgtcacac tcgctaaaaa cactaatcac
    13681 acggaaatct gttttcaata aaaactccaa ctttaactca ttttcaattt caactgaaag
    13741 attttttcat tagagaatgt ctagaactag gccgttt
//
