LOCUS       sequence-95469-e       10116 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  drives mCherry in C. elegans neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10116)
  AUTHORS   Armenti ST, Lohmer LL, Sherwood DR, Nance J
  TITLE     Repurposing an endogenous degradation system for rapid and targeted 
            depletion of C. elegans proteins.
  JOURNAL   Development. 2014 Dec;141(23):4640-7. doi: 10.1242/dev.115048. Epub 
            2014 Nov 5.
  PUBMED    25377555
REFERENCE   2  (bases 1 to 10116)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.115048"; journalName: "Development"; date: "2014-12"; 
            volume: "141"; issue: "23"; pages: "4640-7"
COMMENT     Sequence Label: pSA102
FEATURES             Location/Qualifiers
     source          1..10116
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2782..2799)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2782..2798)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4506..4525)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4625..4647
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4685..4703)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4771..4875
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4876..4944,4945..5736)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5094..5113)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5907..6495
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6396..6415
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8131..8142)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 atcttcagat gggagcagtg gactgtctat ttgatattct taaaatgtac tctataaaag
       61 atgaaactgt aatgaccaat tccctacata aatattttta actaattggt cggatatttg
      121 gggatcagag aagtacttgt ttttgcgata ttaataatga tatatttttc tagtaaaaaa
      181 atgttaagcg atttgtgaat aattaagttg gcttgaaaaa gattttttga tagctatgta
      241 ttttgttaat ttaaatcaga ttttaaaact ttattattga ctatttatat atattatata
      301 ttatatattt tcatggttta atcaaaatct attaaattat ttctaaaatc tctaaatatc
      361 gaattctcta aaaagcttca tcaacaaaag catttcaatt atctaacaca atgagaataa
      421 ttcaagaact gaaatagaaa gaaatctaga aaaaaaagag agtgaggtgc cctattaaat
      481 cggtgctctc ctctctaaca gaagcgccta cacgcaccca agagagctct ttttctttct
      541 tttttcggag cacacataca cgcatacact cctcagaagt gtatagagaa aatgggagaa
      601 gaagcgaaag aagacgaaga agcggttgtg atgtatgcat cgggcgtttc tatctctctc
      661 cgtgagcaac gagctagtca acccatcgat ccatttttgt gagcacacac agagagagac
      721 tcaaacacac tcacagcttc acactgagct ccaagagctc tgtagagaga agccagagta
      781 tgctttcctc gattttccta acgtcttctt cttctgcttt tttctttttc ccaacgagtt
      841 gtagtccact aacttcaatt tttcctccag agaaagagtt ttagagagac agctgataaa
      901 tacactagag aaataaaaag aatgataaaa atttgctatt ttaatcagtt ccctctcgtt
      961 tctctaagtt ttgttgttgg gttgggaaat taaaattgga aaatggaaat aacgtttttt
     1021 tctgtctaat cttgaattct ccagacattt cttatttcct tttttgttgc aagtttttct
     1081 tccttctcta atttttaatc aaagatatct cgctatgaac tttgaatttt tgatattttg
     1141 attctataaa tacataaagt aatctaaatg ctcttttaaa ataaatctac agtagcccta
     1201 ttttcagatg gtctcaaagg gtgaagaaga taacatggca attattaaag agtttatgcg
     1261 tttcaaggtg catatggagg gatctgtcaa tgggcatgag tttgaaattg aaggtgaagg
     1321 agaaggccga ccatatgagg gaacacaaac cgcaaaacta aaggtaagtt taaacatata
     1381 tatactaact aaccctgatt atttaaattt tcaggtaact aaaggcggac cattaccatt
     1441 cgcctgggac atcctctctc cacagttcat gtatggaagt aaagcttatg ttaaacatcc
     1501 ggcagatata ccagattatt tgaaactttc attcccggag ggttttaagt gggaacgcgt
     1561 aatgaatttt gaagacggag gagttgttac agtgacgcaa gactcaaggt aagtttaaac
     1621 agttcggtac taactaacca tacatattta aattttcagc ctccaagatg gagaatttat
     1681 ttataaagtc aaacttcgag gaacgaattt cccctcggat ggacctgtta tgcagaagaa
     1741 gactatggga tgggaagctt caagtgaaag aatgtaccct gaagacggtg ctcttaaggg
     1801 agagattaaa caacgtctta aattgaaaga tggaggacat tacgatgctg aggtaagttt
     1861 aaacatgatt ttactaacta actaatctga tttaaatttt caggtgaaga caacttacaa
     1921 agccaaaaaa ccagttcagc tgccaggagc gtacaatgtt aatattaaac tggatatcac
     1981 ctcccacaac gaggattaca ctatcgttga gcaatatgaa agagctgaag ggcggcactc
     2041 gacaggtggc atggatgaat tgtataagta ggtccaatta ctcttcaaca tccctacatg
     2101 ctctttctcc ctgtgctccc accccctatt tttgttatta tcaaaaaaac ttcttcttaa
     2161 tttctttgtt ttttagcttc ttttaagtca cctctaacaa tgaaattgtg tagattcaaa
     2221 aatagaatta attcgtaata aaaagtcgaa aaaaattgtg ctccctcccc ccattaataa
     2281 taattctatc ccaaaatcta cacaatgttc tgtgtacact tcttatgttt tttttacttc
     2341 tgataaattt tttttgaaac atcatagaaa aaaccgcaca caaaatacct tatcatatgt
     2401 tacgtttcag tttatgaccg caatttttat ttcttcgcac gtctgggcct ctcatgacgt
     2461 caaatcatgc tcatcgtgaa aaagttttgg agtatttttg gaatttttca atcaagtgaa
     2521 agtttatgaa attaattttc ctgcttttgc tttttggggg tttcccctat tgtttgtcaa
     2581 gagtttcgag gacggcgttt ttcttgctaa aatcacaagt attgatgagc acgatgcaag
     2641 aaagatcgga agaaggtttg ggtttgaggc tcagtggaag gtgagtagaa gttgataatt
     2701 tgaaagtgga gtagtgtcta tggggttttt gccttaaatg acagaataca ttcccaatat
     2761 accaaacata actgtttaaa cactggccgt cgttttacac tcgagacgta cggtgcgcgc
     2821 gatgcattcg aagatctgcc cactagtgag tcgtattata agtgcaagta agatcagtgt
     2881 ttgtttcgat atcagtctgt ttcgtaacgg tcttctgtat aactacaaaa aagaataaaa
     2941 aaccgtatca caactgagaa aaaagacaaa acttattgaa cagtgttcct ttcaaaaaat
     3001 tattttgtaa aaaatcgagt ttttctccaa aataacgcac gcaatcaaaa acaagaaaac
     3061 taacgtgttc tcctgggcgt ggaactcctt atcaagatta ccattattgt ctgtcagaat
     3121 tgatatttca attatttcat atctagacaa acccgggtgt cttgcattta ttagtactgt
     3181 gagtttacat acaaacgagg cttactcaga taacatagag tcacctctaa ttagcaaaaa
     3241 cccatttcta attagctatt tcgaccagtt ctaaagtcac cgtaattaga aatggttttt
     3301 gtagcttttc taattagaaa tgaccccgag ataagtgaaa tatggcattt tctgggtttt
     3361 ctagtaagaa atgatcccgc acgtagtgat tccgcgttac aagccaaatt caaattaaaa
     3421 aagtatatta cacaattcaa gttacattat tttcaggaaa caatggcgaa gagcaaagca
     3481 gagattcaaa aggaggaaag agacaacaga tatgaacgaa gacttcttga aaagagaaag
     3541 agacatgagg tgagtaacca tgataataaa taatcaagta agggctatag atttataaat
     3601 tctggagtta acagtttcga aaaaaagtag ttttcatatg tgcgaaggaa tatcaaaaat
     3661 aggggaatag cctaaacgag tgagaaaatt aaatgtttgt tattttttca aaaatgtctg
     3721 cagggccgtt atcctttcaa atgcactcta tcaaacatag atcatatcca tcatatctta
     3781 tccagaattt atatcatatc caagattgag agcgtttcaa ggaaaatctc aaacaaccat
     3841 acaattttgt agatcgacaa caaggcctcc acttcttcat cgatggcctc tgcagcacct
     3901 gcaccaccaa gtttgtattt ttcatattaa tataaagtga gccacgcata gtcgttttta
     3961 ggatttttgc cagatcgatg tcgcgcaaac agactgtttt tttttctttg aaagccgtgc
     4021 gtgacatggg ccacggattg taacgccgcg ctggatggat aaaaatttat tcagtttttt
     4081 agcctttaca aaatatggtt cgactacttt ctttattttt acatttttat aggtgctcat
     4141 tgagctgaga tattttgggt ataatcggaa aatcgctgat tttggtcatt tttctgcact
     4201 aaagattaca caaaaagttc agtatttaga ttcattgttg aaaattgggg atgatctaca
     4261 gatacatatc gccgtgttca aaaacacacg tgctgtgacg tcgtgactgg ggaaaaccct
     4321 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     4381 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc
     4441 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact
     4501 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
     4561 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     4621 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
     4681 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     4741 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     4801 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     4861 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     4921 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     4981 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     5041 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     5101 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     5161 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     5221 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     5281 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     5341 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     5401 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     5461 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     5521 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     5581 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     5641 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     5701 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     5761 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     5821 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     5881 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     5941 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     6001 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     6061 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     6121 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     6181 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     6241 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     6301 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     6361 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     6421 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     6481 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggt cttttgctgg
     6541 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     6601 gcctttgagt gagctgatac cagcggataa caatttcaca gggccctgtc ggattatggg
     6661 ctcttctttt atcctctact ttctttctag aaaatgcaaa tgttgagtca gaaattgctg
     6721 aatggagtac aactgcaatg ggtttagctt atgtttctgg aacaattact ggtggaattg
     6781 tgattgaaaa gtaaggttat atttcatttc aataaatgtt accacagtat aacaaaaaca
     6841 agttacattg ttcagagtat gtctgaatgt attattatta ttgtatttgt gcatcagtac
     6901 tgtagttgta ctgaagaagg gtttaaaata atctcaacgt ttttaagacg agttagtgag
     6961 aatacggtcg cagttaggat gtttggacat agttttatta gataatcgtg aactaactca
     7021 ctataaaaac ccgctaactc attgaataag atagttttaa acgttcaaaa tttgaagaca
     7081 aaattgtatg aataagttta cagagttggt cgacgcaagc ttctccttct cttcacattt
     7141 ctcaataatt tagctctaat tgcattcgta ttcttcgcaa aaatacgtat tctgattgac
     7201 ccgatgaaat acggatgcct agttgcattg atcatctacg gttatactta tgggtgaggt
     7261 ttttcaaata tattttgatt taatcgaata atgaaatttc agaactggag ttggtccaat
     7321 ttcgtggttt atatcctctg aactggtacc tcaaaaacat cgtagtattg ctcaatctgt
     7381 ggcatattca atgaatactg taatggtggt tatcagtaca tttacagtat tacctctata
     7441 ttccgttatt ggtagttatg ctttccttat tctatattca attccttcat ttataagtat
     7501 gcttatatta tttcgctact taccggaaac caaaggacga gagattcatg atattgtgaa
     7561 tgaacttaaa aaaaaatgaa aacctttgaa aacgttcata cggccgaaat tttacttgat
     7621 cgagccaatt gaacttttgt taagacttac agaaaagtga gagattttgt tacaaaaaag
     7681 tgagaaaaaa gcgaaaatga acaggaaaaa gaagaaaaat acacccgaag tatcatataa
     7741 aaattaaaaa gattgtttga cctggcggaa cttttttgca aaaactctga atatccatgg
     7801 cacatgcttc gtgcaaaaca ggatccaaat gaatatcaac aagagattct tgaagacgac
     7861 gagccacttg ctcagagcat tgcttatctc gaatgagacc cttgttaaaa tcatttttca
     7921 aacattatat gacattgtcg aaatgtcctc ctgattccat gatggtagca aactcacttc
     7981 gtctttgagc caatttatcc aagtccttgt aaaaagtatt cgaaaattgt taacggataa
     8041 aatgtttatt ataatatcaa aaacaatttg tcagttgacc actttttgat atatttgaca
     8101 gaaaccggga tgaattggct caaagttagg gcgcccttct attacagggt ttctgataac
     8161 aaaccggtta ttaactccca acaagggatg atttcaattc atcatgctca aattgaccca
     8221 aattaagtta catgacaaat tcatcgccct tttcaactct tcttggctca tcatctgtta
     8281 ttctgttcct attctgtctg caccctatac ccctttgcat actctctcgt cattcctttc
     8341 ttggatagtg tcttcttctc cccagctctg ctacttctat gacttgcgcc cgctgctttt
     8401 ccgctcgttc ctctcctctc tgacgtcttc ttctgcttct tttctccttc ctttttccct
     8461 tctcaatctt cctctttttc ccatttctgt caatcattcg aagaagaaga agaagaagac
     8521 cctcattcat ttcatttttt tttctgtcgg tgtgtgctgc tcggttaaga gtgagctctc
     8581 tattccacgt cttcttcttt ttcttcttcg attcgaatca atcactccac caaaacgcat
     8641 tcgtttttgg gattcacccc gcggttcgca ataggtttct ttttcaaatt attagcgtta
     8701 taaatagaaa aatggtggag tttcaataaa aatgataatt tacaaagtgt attttgatta
     8761 catgtactca aaaaggttga aatttttcat accagttttc cggaaatcca tctgatatca
     8821 ttatcgtatt tttcttttta aaaatgtttt caaaaaaaaa caaaatatag ctggttattt
     8881 ggcaccctct aattaccatt ttcctgtcac accacactct tttccttctt ccacttcttt
     8941 ccgttttcag ccgcttccaa ccaaaccgat atgaaagccg agcaacaacg atcgattcca
     9001 cccggctcgg cgaccttccc gtcgcaggtg agactcagaa aactagagaa accgctcaac
     9061 taactcttga tatccgattt cattcttttc tcttttcttt ttgttgaact tttccatatt
     9121 tccagatgcc acggccacca ccaagcaccg aacaaggaat cacaacggaa tcggagcttg
     9181 cgaagaaagc tcaaatcact ccgaacgacg ttttagcact tccgggaatc actcaaggta
     9241 tcttgctccc tttggatttc agtttaaaac ataaatttag gattcttatg ctccccatct
     9301 gcgaacatct ataacatcga gttcaccaag ttccaaatcc gtgatctgga cactgagcaa
     9361 gtgcttttcg agatcgccaa accggagaac gatcaggaga atgatgagtc gccacaggag
     9421 tcggcaagat acgtgcgtta tagatttgct ccaaactttt tgaaactcaa aacggtcgga
     9481 gcaactgtgg aattcaaagt aggagacatc ccaatccatc atttccgaat gatcgaacgt
     9541 cacttcttca aagatcgcct tctgaagtgt tttgactttg aattcggatt ctgtattccg
     9601 aattcacgaa acaactgtga acatatctat gagttccctc aactctctca acaactcagt
     9661 gagtcattat tctaaaaagt acaattcaaa agactaatct ctttttcagt ggacgacatg
     9721 atcaacaatc caaacgagac tcgttctgac agcttctatt tcgtcgataa caaactcgtc
     9781 atgcacaaca aagccgacta ctcatatgat gcataaatat ttaatacaaa aatgttctgg
     9841 ataattattc tgtcgaatag aaaaaaaact ccaaatgtga ttaaattcca ataattcctg
     9901 tctagtttgt tccttcccct tccccttctc atgttcaatg cattcctaag cttttcagtt
     9961 ccccccttgt ttctatattt tttcgctgtc ctgtcacact cgctaaaaac actaatcaca
    10021 cggaaatctg ttttcaataa aaactccaac tttaactcat tttcaatttc aactgaaaga
    10081 ttttttcatt agagaatgtc tagaactagg ccgttt
//
