LOCUS       sequence-95488-e        1125 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses sigma32 I54N mutant in E. coli to render a heat-shock like
            cellular folding environment.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Zhang X, Liu Y, Genereux JC, Nolan C, Singh M, Kelly JW
  TITLE     The Heat-Shock Response Transcriptional Program Enables High-Yield 
            and High-Quality Recombinant Protein Production in Escherichia coli.
  JOURNAL   ACS Chem Biol. 2014 Jul 22.
  PUBMED    25051296
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2014 Jul 22."
COMMENT     Sequence Label: pBAD.Sigma32.I54N
FEATURES             Location/Qualifiers
     source          1..1125
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(969..986)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 ctactgacgc tttttatcgc aactctctac tgtttctcca tacccgtttt ttgggctaac
       61 aggaggaatt aaccatgact gacaaaatgc aaagtttagc tttagcccca gttggcaacc
      121 tggattccta catccgggca gctaacgcgt ggccgatgtt gtcggctgac gaggagcggg
      181 cgctggctga aaagctgcat taccatggcg atctggaagc agctaaaacg ctgaacctgt
      241 ctcacctgcg gtttgttgtt catattgctc gtaattatgc gggctatggc ctgccacagg
      301 cggatttgat tcaggaaggt aacatcggcc tgatgaaagc agtgcgccgt ttcaacccgg
      361 aagtgggtgt gcgcctggtc tccttcgccg ttcactggat caaagcagag atccacgaat
      421 acgttctgcg taactggcgt atcgtcaaag ttgcgaccac caaagcgcag cgcaaactgt
      481 tcttcaacct gcgtaaaacc aagcagcgtc tgggctggtt taaccaggat gaagtcgaaa
      541 tggtggcccg tgaactgggc gtaaccagca aagacgtacg tgagatggaa tcacgtatgg
      601 cggcacagga catgaccttt gacctgtctt ccgacgacga ttccgacagc cagccgatgg
      661 ctccggtgct ctatctgcag gataaatcat ctaactttgc cgacggcatt gaagatgata
      721 actgggaaga gcaggcggca aaccgtctga ccgacgcgat gcagggtctg gacgaacgca
      781 gccaggacat catccgtgcg cgctggctgg acgaagacaa caagtccacg ttgcaggaac
      841 tggctgaccg ttacggcgtt tccgctgagc gtgtacgcca gctggaaaag aacgcgatga
      901 aaaaattgcg tgctgccatt gaagcgtaat agaagcttgg tgtttggcgg atgagagaag
      961 attttcagcc tgatacagat taaatcagaa cgcagaagcg gtctgataaa acagaatttg
     1021 cctggggcag ttagcggggc ccacctgacc catgccgaac tcagaaggaa acgccgtacc
     1081 gagggggtcc cccatgaata taaacgcaaa cgttctcggc gagga
//
