LOCUS       sequence-95496-e         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate entry vector at position 1, no 5' ATG, LacZ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Kirchmaier S, Lust K, Wittbrodt J
  TITLE     Golden GATEway cloning--a combinatorial approach to generate fusion 
            and recombination constructs.
  JOURNAL   PLoS One. 2013 Oct 7;8(10):e76117. doi: 
            10.1371/journal.pone.0076117.
  PUBMED    24116091
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-7"; volume: "8(10):e76117. doi"; pages: " 
            10.1371/journal.pone.0076117"
COMMENT     Sequence Label: pGGEV_-1_XcmI-LacZ
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        36..54
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     CDS             complement(92..475)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSYLGDTIEYSSYASNALGALPYGRPAGGREFTSDIEFPR
                     PPWRPGACDVGPNSPYSESYYNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEA
                     RTDRPSQQLRSLNGEWTRPVAAH"
     primer_bind     241..263
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     255..272
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     256..272
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     279..298
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        279..297
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      328..337
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      332..341
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(435..453)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(471..487)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(471..487)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(484..506)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    495..511
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(519..525,526..543,544..549))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    564..585
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(702..719)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ccgccattct gcctggggac gtcggagcaa gcttgattta ggtgacacta tagaggtctc
       61 agtatccgga tccagcggtg gcgtggcgcg cttaatgcgc cgctacaggg cgcgtccatt
      121 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
      181 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
      241 cccagtcacg acgttgtaaa acgacggcca gtgaattgta atacgactca ctatagggcg
      301 aattgggccc gacgtcgcat gctcccggcc gccatggcgg ccgcgggaat tcgatatcac
      361 tagtgaattc gcggccgcct gcaggtcgac catatgggag agctcccaac gcgttggatg
      421 catagcttga gtattctata gtgtcaccta aatagcttgg cgtaatcatg gtcatagctg
      481 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
      541 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
      601 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
      661 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
      721 cgctcgccac gccaccgctg gtaccggctt gagaccctca tcctcccggg taactaagta
      781 aggatctcga ccgatcctga gaacttcagg gtgagtttgg ggacccttga ttgttctttc
      841 tttttcgcta ttgtaaaatt catgttatat ggagg
//
