LOCUS       sequence-95497-e         749 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate entry vector at position 1, no 5' ATG, LacZ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Kirchmaier S, Lust K, Wittbrodt J
  TITLE     Golden GATEway cloning--a combinatorial approach to generate fusion 
            and recombination constructs.
  JOURNAL   PLoS One. 2013 Oct 7;8(10):e76117. doi: 
            10.1371/journal.pone.0076117.
  PUBMED    24116091
REFERENCE   2  (bases 1 to 749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-7"; volume: "8(10):e76117. doi"; pages: " 
            10.1371/journal.pone.0076117"
COMMENT     Sequence Label: pGGEV_-1_XcmI-LacZ
FEATURES             Location/Qualifiers
     source          1..749
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          42..614
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     706..723
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aaatgatgag acagcacaac aaccagcacg ttgcccagga gctgtaggaa agagaagaag
       61 gcatgaacat ggttagcaga ggggcccggt ttggactcag agtattttat cctcatctca
      121 aacagtgtat atcattgtaa ccataaagag aaaggcagga tgatgaccag ggtgtagttg
      181 tttctaccaa taagaatatt tccacgccag ccagaattta tatgcagaaa tattctacct
      241 tatcatttaa ttataacaat tgttctctaa aactgtgctg aagtacaata taatataccc
      301 tgattgcctt gaaaaaaaag tgattagaga aagtacttac aatctgacaa ataaacaaaa
      361 gtgaatttaa aaattcgtta caaatgcaag ctaaagttta acgaaaaagt tacagaaaat
      421 gaaaagaaaa taagaggaga caatggttgt caacagagta gaaagtgaaa gaaacaaaat
      481 tatcatgagg gtccatggtg atacaaggga catcttccca ttctaaacaa caccctgaaa
      541 actttgcccc ctccatataa catgaatttt acaatagcga aaaagaaaga acaatcaagg
      601 gtccccaaac tcaccctgaa gttctcagga tcggtcgaga tccttactta gttacccggg
      661 aggatgaggg tctcaagccg gtaccagcgg tggcgtggcg agcgcagcga gtcagtgagc
      721 gaggaagcgg aagagcgccc aatacgcaa
//
