LOCUS       sequence-95500-e        1083 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human Kras with a G12C mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1083)
  TITLE     Mutant Ras Constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBabe-Kras G12C
FEATURES             Location/Qualifiers
     source          1..1083
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             22..48
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             28..54
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             76..642
                     /codon_start=1
                     /product="human small GTPase proto-oncoprotein"
                     /label=K-Ras
                     /translation="MTEYKLVVVGACGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVK
                     DSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREI
                     RKHKEKMSKDGKKKKKKSKTKCVIM"
     primer_bind     complement(654..674)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        659..988
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      839..974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(998..1017)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 gccgggatca tggcttcaag ttatccatat gatgtgcccg actatgcctc acttggagga
       61 ccctctggat ccaccatgac tgaatataaa cttgtggtag ttggagcttg cggcgtaggc
      121 aagagtgcct tgacgataca gctaattcag aatcattttg tggacgaata tgatccaaca
      181 atagaggatt cctacaggaa gcaagtagta attgatggag aaacctgtct cttggatatt
      241 ctcgacacag caggtcaaga ggagtacagt gcaatgaggg accagtacat gaggactggg
      301 gagggctttc tttgtgtatt tgccataaat aatactaaat catttgaaga tattcaccat
      361 tatagagaac aaattaaaag agttaaggac tctgaagatg tacctatggt cctagtagga
      421 aataaatgtg atttgccttc cagaacagta gacacaaaac aggctcagga cttagcaaga
      481 agttatggaa ttccttttat tgaaacatca gcaaagacaa gacagggtgt tgatgatgcc
      541 ttctatacat tagttcgaga aattcgaaaa cataaagaaa agatgagcaa agatggtaaa
      601 aagaagaaaa agaagtcaaa gacaaagtgt gtaattatgt aagtcgaccc tgtggaatgt
      661 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
      721 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
      781 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
      841 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
      901 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
      961 cttttttgga ggcctaggct tttgcaaaaa gcttaccatg accgagtaca agcccacggt
     1021 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     1081 cga
//
