LOCUS       sequence-95506-e         329 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses alpha subunit of hERG1a K channel in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Mitcheson JS, Chen J, Lin M, Culberson C, Sanguinetti MC
  TITLE     A structural basis for drug-induced long QT syndrome.
  JOURNAL   Proc Natl Acad Sci U S A. 2000 Oct 24;97(22):12329-33.
  PUBMED    11005845
REFERENCE   2  (bases 1 to 329)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2000-10-24"; volume: "97(22)"; pages: 
            "12329-33"
COMMENT     Sequence Label: pCEP4-hERG1a
FEATURES             Location/Qualifiers
     source          1..329
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        54..153
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     104..124
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     150..174
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ggggatttcc aagtctccac cccattgacg ttcaatggga gtttgttttg gcaccaaaat
       61 caacgggact ttccaaaatg tcgtaataac cccgccccgt tgacgcaaat gggcggtagg
      121 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatctctaga
      181 agctgggtac cagctgctag caagcttctc aggatgccgg tgcggagggg ccacgtcgcg
      241 ccgcagaaca ccttcctgga caccatcatc cgcaagtttg agggccagag ccgtaagttc
      301 atcatcgcca acgctcgggt ggagaactg
//
