LOCUS       sequence-95518-e         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral GFP reporter for testing enhancer activity in different 
            cell types. Insert is an enhancer identified in the exonic region of
            the keratin type II cluster. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  TITLE     Yu Lab-keratin enhancer GFP reporters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV-Enh eGFP Reporter-muKC2-X5
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    135..159
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             435..452
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
       61 aaaacaaatt acaaaaattc aaaattttat cgatcacgag actagcctcg agaagcttga
      121 tatcgaattc catcacaagt ttgtacaaaa aagcaggctt tggatgcctc caggtgggca
      181 caccgggaag ccagctcccc catagccacc accaaagcca gctccaccac cgaagccaaa
      241 gccactacca gctccaccac caaaaccaaa tccactgccg gctcctcctc caaagccaaa
      301 gccaccagca ccaaattggt tcctgaagct gcctccgcca gaactgtagg atatcctttt
      361 ggagcccccc acattgtaca ggctccggct gccatagccc ccggctccac aagcaccccc
      421 aaggctgatc ctgccaccac caccaccacc actgcgggac accgagctga agctcgtacg
      481 agagacagat ggggtgatgg cagaggcggc gctgaaagag cggctgcccc cacttcggaa
      541 ggacacactg gactggcgag acatgatggt tcctggagga gcaagaacac tgggcgctgg
      601 tggtggggag gtgctggcga ggtcagcggc gttcaacagg acgctgtggg tggctctgta
      661 acttaggaga tttgaggtcc ccttttatcc ccttagaagt gggttgggca gaggagctgt
      721 cgagctgtga atgattagtg ggctgggcat gcctgggggg cagctgtgag ctcacccagc
      781 acacctgcac agtggtgt
//
