LOCUS       sequence-9553-ex        6103 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6103)
  AUTHORS   Klotzsche M, Ehrt S, Schnappinger D
  TITLE     Improved tetracycline repressors for gene silencing in mycobacteria.
  JOURNAL   Nucleic Acids Res. 2009 Apr;37(6):1778-88. doi: 10.1093/nar/gkp015. 
            Epub 2009 Jan 27.
  PUBMED    19174563
REFERENCE   2  (bases 1 to 6103)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkp015"; journalName: "Nucleic Acids Res"; date: 
            "2009-04"; volume: "37"; issue: "6"; pages: "1778-88"
COMMENT     Sequence Label: pTE-10M0X
FEATURES             Location/Qualifiers
     source          1..6103
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     38..54
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     39..55
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(1056..1083)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1173..1190)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1344..1932)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1424..1443)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgttctcg gctcgatgat ccccggcggg ccccccctcg aggtcgacgg tatcgattta
       61 attaatatgc atcaattgat ttaaatacta gtgttaacta tttaattggg gaccctagag
      121 gtcccctttt ttattttaaa aattttttcn aaggggacct ctagggtccc cagctggcta
      181 gcgagtcatg aggtnaccat ggtcattagg agccgctctc gcacttcagc tgcttctcca
      241 agccgcagat gatcagctcc aggccgaaca agaaggccgg ctcggcgccc tggtggtcga
      301 acagctcgat ggcctggcgc agcaacggcg gcatgctgtc ggtggtcggg gtctcgcgct
      361 cctccttggc gacctggtgc tcctggtcct ccaacacgca gcccagggtg aagtggccca
      421 cggccgacag ggcgtacaac gcgttctcca gcgagaagcc ctgctggcac aggaaggcca
      481 actggttctc cagggtctcg tactgcttct cggtcggccg ggtgcccagg tgcaccttgg
      541 cgccgtcgcg gtggctcagc aaggcgcagc ggaagctctt ggcgttgttg cgcaggaagt
      601 cctgccagct ctcgccctcc agcgggcaga agtgggtgtg gtggcggtcc aacatctcga
      661 tggccagggc gtcgagcagg gcccgcttgt tcttgacgtg ccagtacagg gtcggctgct
      721 cgacgcccag cttctgggcc agcttgcggg tggtcaggcc ctcgatgccg acctcgttca
      781 gcagctccag ggcggagttg atgaccttgg acttgtccag gcgggacatt gcgaagtgat
      841 tcctcctgat ggtaccggat ccgtggcagg agggtcgcct gcgtcggcaa ctcgcccagg
      901 ctacacgaga gccctcgact gcgaaaatgc cgaccaggca ccaccgcact ccaggcttaa
      961 cccggtgcga cgatccaata ttacgagatc tgatatcctc gaggcatgcc acgtgcctgc
     1021 aggtttaaac gcggccgcaa acagtattaa acgcaaaaag gccatccgtc aggatggcct
     1081 tctgcttagc taatcaacta gttctagctg cattaatgaa tcggccaacg cgcggggaga
     1141 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     1201 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     1261 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     1321 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     1381 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     1441 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     1501 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     1561 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     1621 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     1681 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     1741 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     1801 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     1861 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     1921 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     1981 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     2041 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     2101 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     2161 atagttgcct gactccccgg acgtggccga ccagcccgtc atcgtcaacg cctgaccgcg
     2221 gtgcggacag gccgtgtcgc gaccggccgt gcggaattaa gccggcccgt accctgtgaa
     2281 tagaggtccg ctgtgacaca agaatccctg ttacttctcg accgtattga ttcggatgat
     2341 tcctacgcga gcctgcggaa cgaccaggaa ttctgggagc cgctggcccg ccgagccctg
     2401 gaggagctcg ggctgccggt gccgccggtg ctgcgggtgc ccggcgagag caccaacccc
     2461 gtactggtcg gcgagcccga cccggtgatc aagctgttcg gcgagcactg gtgcggtccg
     2521 gagagcctcg cgtcggagtc ggaggcgtac gcggtcctgg cggacgcccc ggtgccggtg
     2581 ccccgcctcc tcggccgcgg cgagctgcgg cccggcaccg gagcctggcc gtggccctac
     2641 ctggtgatga gccggatgac cggcaccacc tggcggtccg cgatggacgg cacgaccgac
     2701 cggaacgcgc tgctcgccct ggcccgcgaa ctcggccggg tgctcggccg gctgcacagg
     2761 gtgccgctga ccgggaacac cgtgctcacc ccccattccg aggtcttccc ggaactgctg
     2821 cgggaacgcc gcgcggcgac cgtcgaggac caccgcgggt ggggctacct ctcgccccgg
     2881 ctgctggacc gcctggagga ctggctgccg gacgtggaca cgctgctggc cggccgcgaa
     2941 ccccggttcg tccacggcga cctgcacggg accaacatct tcgtggacct ggccgcgacc
     3001 gaggtcaccg ggatcgtcga cttcaccgac gtctatgcgg gagactcccg ctacagcctg
     3061 gtgcaactgc atctcaacgc cttccggggc gaccgcgaga tcctggccgc gctgctcgac
     3121 ggggcgcagt ggaagcggac cgaggacttc gcccgcgaac tgctcgcctt caccttcctg
     3181 cacgacttcg aggtgttcga ggagaccccg ctggatctct ccggcttcac cgatccggag
     3241 gaactggcgc agttcctctg ggggccgccg gacaccgccc ccggcgcctg acgccccggg
     3301 ccgcccggcg ccgcccccgg cccccggcgg ccggccgccc ggagccccgc ccgcgctcgg
     3361 gagccccggg cccgcgccga agcccgctgc tgcgagcccg gagcgggccg gccgacggcg
     3421 gtgcgggccc ggcggcggac gctcagcagc ggcgggcgtg aaaggccctg gcatcctcga
     3481 tcatctcctc cagggtggtc ggccgagctt ccatcccagc tcggcaagga tcaacgagga
     3541 cagtcgcacg acgaagttct tctggatcgc gcccgtgctg gaagcactca acctcgaagc
     3601 gtgtggttgc ggagccatct agcaaccaca cgaaacatgc gcaacgaacc gcgcaacgaa
     3661 caacgcctag aactggccct agatgagctg actcgtatcg ttggtaaacc tagtttgacc
     3721 agcatgtttt aactacgttc ggtgagctgt caacggggcc tgtaacggca caacgaaccg
     3781 tgcaacgaga gtggccacgg atgccaccac aggcactaca acggagttcg ccacgtacat
     3841 caccacaacc accgattctg gcggtgagct ccccgatatt cagcggaaat ggcttggtat
     3901 cgaccaagat tcgtagaacc ccgtctcgtc tggctggtat tcaaaacggg cgcaacgaaa
     3961 cacgcaacga gacaggcatg gcccaaacca gaaaactagc gtctaccagg acttttacct
     4021 gtccgacccg ttgcaacgga accccccacg gaacccccgc gacacccgct ccccaattgc
     4081 gttagaacag cggtggattg tcggcttcgt tgtgggcctt ttgagccgct tcctgttctg
     4141 ccgcacgctc tttcctcgcc cgatagccga gtcgcttaac ggtgtccaga tgcagcccga
     4201 aatgtttggc cgtttgcggc caagagtggc cctcgtcgtc gtgataggcg cggatgcgtt
     4261 cgcggcgtgc agcctgctcg gcgagccact cgctgcgttc ctgcgccacg agccggacga
     4321 cgtggcgttc ggatagtccg gtgattcgag cgccttcggc ggcggtcacg cgccgctttt
     4381 tgcggacagt cggctgccgg ttgtagccgt cgctgtagcc gtcgctgtag ccgtcgctca
     4441 tagcaatgcc tccatggctg acgcggactt tgcgcgccgc gcaactgtgc tcgccgccgt
     4501 gcgcgctgct gcgcccttcc gcgagatggc cgactggcgc gcactgagtg tggcctcgta
     4561 gaccacgatc ccgtccgccc aaatgcgcga cttggttgtg atccaacgcc aaatgctgtt
     4621 ggcgatggcg cggacctcgc tgtccggtag cggtccggga cacacgtcgt tgcacgggaa
     4681 ttcggcgttt cgcgcgtggc actcggcata gatcgcgcgg ccgagtccgt ccacgttccg
     4741 ggtcggcagg tagatccgca tgagggcggg acgataggcc cacaacctga cggaatcgaa
     4801 cagtgcgcaa ttccgcccta gcggcgtcgg agccgctttg tacgtggtct gctgacgcca
     4861 gcgcggcggt ggcatgttcg cgccgagctc ggcctcgatg tggctgagtg tgtagagatc
     4921 tgagtggagc cattccgttt cccaggcgat gtggccgggg tttttggtca tgaggcctga
     4981 gtaactgcgg tcgccgtcga cggcgcgccg aaggccttcg gcgcacgccg ccatgtatgc
     5041 gagcggctta cgccgcgcgt attcggtgcg tggaacaggg gcgttgagtg cccacactgc
     5101 gtgtgcgtgg ccgttggcgc gattgcccac gatcgcgttg ggcagcggat gggacccccg
     5161 ggcgctgagc gctcggagcg ctgcgtctgg atggtctacg tccacgacca gcaggtttgc
     5221 cagcgctgtt gggttcgcct cgatgtaccg gcggcctagg gccgacgcgc ggctttggcg
     5281 gtagatcccc tcgagcagat cgtcgcttgc cagcggccag tacggcagcc agagctgctc
     5341 aaattcgtcg gcgacgtggc tcacgcttgg tagtagacca cgattaatca ccggtgtatg
     5401 gtccgacacg agctccaagt cagatatttc gctgaggggc caccccacaa ctgcacactc
     5461 ccccgctctc ccgtcgagcc ctggtggtgg aacaccagcg acagccgagc acccccaacc
     5521 acctgtacca accaggagga acacatgcgt cgtttcgagg acgtttccgg gccgctgaga
     5581 gccgctgtgg cggccgtaca cgccgcctta gacccgttag accccctgcc gcctgaatgc
     5641 gcgggtacga gccacacagc gcccgaactt acggagctgg tgggctcacc tggctttatg
     5701 gcgtacgaat cggctgtgtg cgacctgttg ggcgaggtga ggtacgcgct actcacgctg
     5761 gcaagggcga cacagccgcc ccaccgagcc cgcacggccg cgcgcggtgt caacaaccgg
     5821 gtgagtcgtg cacaccagca ggtgttcgag gcttggctcg aagtgcagga catcgtggcg
     5881 aacgccgccc gatgagccgc gccttacgct ggctgccagc cgttcgcggg ctggttggtg
     5941 cagcgcgtcg agcggttaga ggccctgcgg tgttccacca ccgcaggcct cgcccttttt
     6001 aaggctgaat ttgcttgtct ccgaatccaa ctggcttgtc caagggtgta tctacgctta
     6061 atccaaagtt caaacgaggg gattacacat gaccaacttc gat
//
