LOCUS       sequence-95534-e         311 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cas9 nuclease and gRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Sakuma T, Nishikawa A, Kume S, Chayama K, Yamamoto T
  TITLE     Multiplex genome engineering in human cells using all-in-one 
            CRISPR/Cas9 vector system.
  JOURNAL   Sci Rep. 2014 Jun 23;4:5400. doi: 10.1038/srep05400.
  PUBMED    24954249
REFERENCE   2  (bases 1 to 311)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2014-06-23"; volume: "4:5400. doi"; pages: " 
            10.1038/srep05400"
COMMENT     Sequence Label: pX330A-1x7
FEATURES             Location/Qualifiers
     source          1..311
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(256..276)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ccggttcttc cgtctggtgt atcttcttct ggcggttctc ttcagccggg tggcctcggc
       61 tgtttcgccg ctgtcgaaca gcagggctcc gatcaggttc ttcttgatgc tgtgccggtc
      121 ggtgttgccc agcaccttga atttcttgct gggcaccttg tactcgtcgg tgatcacggc
      181 ccagcccaca gagttggtgc cgatgtccag gccgatgctg tacttcttgt cagctgctgg
      241 gactccgtgg ataccgacct tccgcttctt ctttggggcc atcttatcgn catcgtcttt
      301 gtaatcaata t
//
