LOCUS       sequence-9555-ex        5146 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5146)
  AUTHORS   Klotzsche M, Ehrt S, Schnappinger D
  TITLE     Improved tetracycline repressors for gene silencing in mycobacteria.
  JOURNAL   Nucleic Acids Res. 2009 Apr;37(6):1778-88. doi: 10.1093/nar/gkp015. 
            Epub 2009 Jan 27.
  PUBMED    19174563
REFERENCE   2  (bases 1 to 5146)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkp015"; journalName: "Nucleic Acids Res"; date: 
            "2009-04"; volume: "37"; issue: "6"; pages: "1778-88"
COMMENT     Sequence Label: pTE-mcs
FEATURES             Location/Qualifiers
     source          1..5146
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(48..75)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(165..182)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(336..924)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(416..435)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(5133..5146,1..3)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     join(5134..5146,1..4)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 atcgatttaa ttaagtttaa acgcggccgc aaacagtatt aaacgcaaaa aggccatccg
       61 tcaggatggc cttctgctta gctaatcaac tagttctagc tgcattaatg aatcggccaa
      121 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
      181 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
      241 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
      301 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
      361 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
      421 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
      481 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
      541 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
      601 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
      661 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
      721 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
      781 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      841 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      901 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      961 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     1021 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     1081 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     1141 tttcgttcat ccatagttgc ctgactcccc ggacgtggcc gaccagcccg tcatcgtcaa
     1201 cgcctgaccg cggtgcggac aggccgtgtc gcgaccggcc gtgcggaatt aagccggccc
     1261 gtaccctgtg aatagaggtc cgctgtgaca caagaatccc tgttacttct cgaccgtatt
     1321 gattcggatg attcctacgc gagcctgcgg aacgaccagg aattctggga gccgctggcc
     1381 cgccgagccc tggaggagct cgggctgccg gtgccgccgg tgctgcgggt gcccggcgag
     1441 agcaccaacc ccgtactggt cggcgagccc gacccggtga tcaagctgtt cggcgagcac
     1501 tggtgcggtc cggagagcct cgcgtcggag tcggaggcgt acgcggtcct ggcggacgcc
     1561 ccggtgccgg tgccccgcct cctcggccgc ggcgagctgc ggcccggcac cggagcctgg
     1621 ccgtggccct acctggtgat gagccggatg accggcacca cctggcggtc cgcgatggac
     1681 ggcacgaccg accggaacgc gctgctcgcc ctggcccgcg aactcggccg ggtgctcggc
     1741 cggctgcaca gggtgccgct gaccgggaac accgtgctca ccccccattc cgaggtcttc
     1801 ccggaactgc tgcgggaacg ccgcgcggcg accgtcgagg accaccgcgg gtggggctac
     1861 ctctcgcccc ggctgctgga ccgcctggag gactggctgc cggacgtgga cacgctgctg
     1921 gccggccgcg aaccccggtt cgtccacggc gacctgcacg ggaccaacat cttcgtggac
     1981 ctggccgcga ccgaggtcac cgggatcgtc gacttcaccg acgtctatgc gggagactcc
     2041 cgctacagcc tggtgcaact gcatctcaac gccttccggg gcgaccgcga gatcctggcc
     2101 gcgctgctcg acggggcgca gtggaagcgg accgaggact tcgcccgcga actgctcgcc
     2161 ttcaccttcc tgcacgactt cgaggtgttc gaggagaccc cgctggatct ctccggcttc
     2221 accgatccgg aggaactggc gcagttcctc tgggggccgc cggacaccgc ccccggcgcc
     2281 tgacgccccg ggccgcccgg cgccgccccc ggcccccggc ggccggccgc ccggagcccc
     2341 gcccgcgctc gggagccccg ggcccgcgcc gaagcccgct gctgcgagcc cggagcgggc
     2401 cggccgacgg cggtgcgggc ccggcggcgg acgctcagca gcggcgggcg tgaaaggccc
     2461 tggcatcctc gatcatctcc tccagggtgg tcggccgagc ttccatccca gctcggcaag
     2521 gatcaacgag gacagtcgca cgacgaagtt cttctggatc gcgcccgtgc tggaagcact
     2581 caacctcgaa gcgtgtggtt gcggagccat ctagcaacca cacgaaacat gcgcaacgaa
     2641 ccgcgcaacg aacaacgcct agaactggcc ctagatgagc tgactcgtat cgttggtaaa
     2701 cctagtttga ccagcatgtt ttaactacgt tcggtgagct gtcaacgggg cctgtaacgg
     2761 cacaacgaac cgtgcaacga gagtggccac ggatgccacc acaggcacta caacggagtt
     2821 cgccacgtac atcaccacaa ccaccgattc tggcggtgag ctccccgata ttcagcggaa
     2881 atggcttggt atcgaccaag attcgtagaa ccccgtctcg tctggctggt attcaaaacg
     2941 ggcgcaacga aacacgcaac gagacaggca tggcccaaac cagaaaacta gcgtctacca
     3001 ggacttttac ctgtccgacc cgttgcaacg gaacccccca cggaaccccc gcgacacccg
     3061 ctccccaatt gcgttagaac agcggtggat tgtcggcttc gttgtgggcc ttttgagccg
     3121 cttcctgttc tgccgcacgc tctttcctcg cccgatagcc gagtcgctta acggtgtcca
     3181 gatgcagccc gaaatgtttg gccgtttgcg gccaagagtg gccctcgtcg tcgtgatagg
     3241 cgcggatgcg ttcgcggcgt gcagcctgct cggcgagcca ctcgctgcgt tcctgcgcca
     3301 cgagccggac gacgtggcgt tcggatagtc cggtgattcg agcgccttcg gcggcggtca
     3361 cgcgccgctt tttgcggaca gtcggctgcc ggttgtagcc gtcgctgtag ccgtcgctgt
     3421 agccgtcgct catagcaatg cctccatggc tgacgcggac tttgcgcgcc gcgcaactgt
     3481 gctcgccgcc gtgcgcgctg ctgcgccctt ccgcgagatg gccgactggc gcgcactgag
     3541 tgtggcctcg tagaccacga tcccgtccgc ccaaatgcgc gacttggttg tgatccaacg
     3601 ccaaatgctg ttggcgatgg cgcggacctc gctgtccggt agcggtccgg gacacacgtc
     3661 gttgcacggg aattcggcgt ttcgcgcgtg gcactcggca tagatcgcgc ggccgagtcc
     3721 gtccacgttc cgggtcggca ggtagatccg catgagggcg ggacgatagg cccacaacct
     3781 gacggaatcg aacagtgcgc aattccgccc tagcggcgtc ggagccgctt tgtacgtggt
     3841 ctgctgacgc cagcgcggcg gtggcatgtt cgcgccgagc tcggcctcga tgtggctgag
     3901 tgtgtagaga tctgagtgga gccattccgt ttcccaggcg atgtggccgg ggtttttggt
     3961 catgaggcct gagtaactgc ggtcgccgtc gacggcgcgc cgaaggcctt cggcgcacgc
     4021 cgccatgtat gcgagcggct tacgccgcgc gtattcggtg cgtggaacag gggcgttgag
     4081 tgcccacact gcgtgtgcgt ggccgttggc gcgattgccc acgatcgcgt tgggcagcgg
     4141 atgggacccc cgggcgctga gcgctcggag cgctgcgtct ggatggtcta cgtccacgac
     4201 cagcaggttt gccagcgctg ttgggttcgc ctcgatgtac cggcggccta gggccgacgc
     4261 gcggctttgg cggtagatcc cctcgagcag atcgtcgctt gccagcggcc agtacggcag
     4321 ccagagctgc tcaaattcgt cggcgacgtg gctcacgctt ggtagtagac cacgattaat
     4381 caccggtgta tggtccgaca cgagctccaa gtcagatatt tcgctgaggg gccaccccac
     4441 aactgcacac tcccccgctc tcccgtcgag ccctggtggt ggaacaccag cgacagccga
     4501 gcacccccaa ccacctgtac caaccaggag gaacacatgc gtcgtttcga ggacgtttcc
     4561 gggccgctga gagccgctgt ggcggccgta cacgccgcct tagacccgtt agaccccctg
     4621 ccgcctgaat gcgcgggtac gagccacaca gcgcccgaac ttacggagct ggtgggctca
     4681 cctggcttta tggcgtacga atcggctgtg tgcgacctgt tgggcgaggt gaggtacgcg
     4741 ctactcacgc tggcaagggc gacacagccg ccccaccgag cccgcacggc cgcgcgcggt
     4801 gtcaacaacc gggtgagtcg tgcacaccag caggtgttcg aggcttggct cgaagtgcag
     4861 gacatcgtgg cgaacgccgc ccgatgagcc gcgccttacg ctggctgcca gccgttcgcg
     4921 ggctggttgg tgcagcgcgt cgagcggtta gaggccctgc ggtgttccac caccgcaggc
     4981 ctcgcccttt ttaaggctga atttgcttgt ctccgaatcc aactggcttg tccaagggtg
     5041 tatctacgct taatccaaag ttcaaacgag gggattacac atgaccaact tcgataacgt
     5101 tctcggctcg atgatccccg gcgggccccc cctcgaggtc gacggt
//
