LOCUS       sequence-95553-e         940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-terminal fusion of EGFP to Drosophila melanogaster Or43a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Benton R, Sachse S, Michnick SW, Vosshall LB
  TITLE     Atypical membrane topology and heteromeric function of Drosophila 
            odorant receptors in vivo.
  JOURNAL   PLoS Biol. 2006 Feb;4(2):e20. Epub 2006 Jan 17.
  PUBMED    16402857
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2006-02"; volume: "4(2)"; pages: "e20. Epub 2006 Jan 
            17"
COMMENT     Sequence Label: RP132 pUAST EGFP:Or43a
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    66..160
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        179..417
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     364..385
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             428..439
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             join(443..445,446..448,449..907)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMA"
     primer_bind     complement(488..509)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(749..768)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 cgcaaagctt ggctgcatcc aacgcgttgg gagctctccg gatccaagct tgcatgcctg
       61 caggtcggag tactgtcctc cgagcggagt actgtcctcc gagcggagta ctgtcctccg
      121 agcggagtac tgtcctccga gcggagtact gtcctccgag cggagactct agcgagcgcc
      181 ggagtataaa tagaggcgct tcgtctacgg agcgacaatt caattcaaac aagcaaagtg
      241 aacacgtcgc taagcgaaag ctaagcaaat aaacaagcgc agctgaacaa gctaaacaat
      301 ctgcagtaaa gtgcaagtta aagtgaatca attaaaagta accagcaacc aagtaaatca
      361 actgcaacta ctgaaatctg ccaagaagta attattgaat acaagaagag aactctgaat
      421 agggaattgg gaattgcaaa acatggtgag caagggcgag gagctgttca ccggggtggt
      481 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
      541 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
      601 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
      661 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
      721 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
      781 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
      841 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
      901 catggccgac aagcagaaga acggcatcaa ggtgaacttc
//
