LOCUS       sequence-9556-ex       12303 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid expressing mouse Oct4, Sox2, Klf4 and cMyc for 
            iPS cell generation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12303)
  AUTHORS   Carey BW, Markoulaki S, Hanna J, Saha K, Gao Q, Mitalipova M, 
            Jaenisch R
  TITLE     Reprogramming of murine and human somatic cells using a single 
            polycistronic vector.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Jan 6. 106(1):157-62.
  PUBMED    19109433
REFERENCE   2  (bases 1 to 12303)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2009 Jan 6. 106(1):157-62."
COMMENT     Sequence Label: FUW-OSKM
FEATURES             Location/Qualifiers
     source          1..12303
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1686..1919
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2104..2148
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2446..2563
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     2622..2638
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2680..2713)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2763..3974
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3811..3830
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             5083..5139
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6118..6171
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7642..7701
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     protein_bind    complement(9103..9136)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    9192..9780
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9245..9265)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9663..9674)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9783..9799)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             10309..10489
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(10551..11139)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10631..10650)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11310..12101,12102..12170))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11933..11952
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12171..12275)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg gccgcgctga tcttcagacc tggaggagga
     1561 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     1621 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     1681 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     1741 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     1801 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1861 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1921 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1981 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     2041 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     2101 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     2161 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     2221 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     2281 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     2341 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca
     2401 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg
     2461 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2521 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2581 cagcagagat ccagtttggt tagtaccggg cccgctctag tcgaggtcga cggtatcgat
     2641 aagctcgctt cacgagattc cagcaggtcg agggacctaa taacttcgta tagcatacat
     2701 tatacgaagt tatattaagg gttccaagct taagcggcct aacccgtgtc ggctccagat
     2761 ctggcctccg cgccgggttt tggcgcctcc cgcgggcgcc cccctcctca cggcgagcgc
     2821 tgccacgtca gacgaagggc gcagcgagcg tcctgatcct tccgcccgga cgctcaggac
     2881 agcggcccgc tgctcataag actcggcctt agaaccccag tatcagcaga aggacatttt
     2941 aggacgggac ttgggtgact ctagggcact ggttttcttt ccagagagcg gaacaggcga
     3001 ggaaaagtag tcccttctcg gcgattctgc ggagggatct ccgtggggcg gtgaacgccg
     3061 atgattatat aaggacgcgc cgggtgtggc acagctagtt ccgtcgcagc cgggatttgg
     3121 gtcgcggttc ttgtttgtgg atcgctgtga tcgtcacttg gtgagtagcg ggctgctggg
     3181 ctggccgggg ctttcgtggc cgccgggccg ctcggtggga cggaagcgtg tggagagacc
     3241 gccaagggct gtagtctggg tccgcgagca aggttgccct gaactggggg ttggggggag
     3301 cgcagcaaaa tggcggctgt tcccgagtct tgaatggaag acgcttgtga ggcgggctgt
     3361 gaggtcgttg aaacaaggtg gggggcatgg tgggcggcaa gaacccaagg tcttgaggcc
     3421 ttcgctaatg cgggaaagct cttattcggg tgagatgggc tggggcacca tctggggacc
     3481 ctgacgtgaa gtttgtcact gactggagaa ctcggtttgt cgtctgttgc gggggcggca
     3541 gttatggcgg tgccgttggg cagtgcaccc gtacctttgg gagcgcgcgc cctcgtcgtg
     3601 tcgtgacgtc acccgttctg ttggcttata atgcagggtg gggccacctg ccggtaggtg
     3661 tgcggtaggc ttttctccgt cgcaggacgc agggttcggg cctagggtag gctctcctga
     3721 atcgacaggc gccggacctc tggtgagggg agggataagt gaggcgtcag tttctttggt
     3781 cggttttatg tacctatctt cttaagtagc tgaagctccg gttttgaact atgcgctcgg
     3841 ggttggcgag tgtgttttgt gaagtttttt aggcaccttt tgaaatgtaa tcatttgggt
     3901 caatatgtaa ttttcagtgt tagactagta aattgtccgc taaattctgg ccgtttttgg
     3961 cttttttgtt agacgaagct tgggctgcag gtcgactcta gagaattcgc catggctgga
     4021 cacctggctt cagacttcgc cttctcaccc ccaccaggtg ggggtgatgg gtcagcaggg
     4081 ctggagccgg gctgggtgga tcctcgaacc tggctaagct tccaagggcc tccaggtggg
     4141 cctggaatcg gaccaggctc agaggtattg gggatctccc catgtccgcc cgcatacgag
     4201 ttctgcggag ggatggcata ctgtggacct caggttggac tgggcctagt cccccaagtt
     4261 ggcgtggaga ctttgcagcc tgagggccag gcaggagcac gagtggaaag caactcagag
     4321 ggaacctcct ctgagccctg tgccgaccgc cccaatgccg tgaagttgga gaaggtggaa
     4381 ccaactcccg aggagtccca ggacatgaaa gccctgcaga aggagctaga acagtttgcc
     4441 aagctgctga agcagaagag gatcaccttg gggtacaccc aggccgacgt ggggctcacc
     4501 ctgggcgttc tctttggaaa ggtgttcagc cagaccacca tctgtcgctt cgaggccttg
     4561 cagctcagcc ttaagaacat gtgtaagctg cggcccctgc tggagaagtg ggtggaggaa
     4621 gccgacaaca atgagaacct tcaggagata tgcaaatcgg agaccctggt gcaggcccgg
     4681 aagagaaagc gaactagcat tgagaaccgt gtgaggtgga gtctggagac catgtttctg
     4741 aagtgcccga agccctccct acagcagatc actcacatcg ccaatcagct tgggctagag
     4801 aaggatgtgg ttcgagtatg gttctgtaac cggcgccaga agggcaaaag atcaagtatt
     4861 gagtattccc aacgagaaga gtatgaggct acagggacac ctttcccagg gggggctgta
     4921 tcctttcctc tgcccccagg tccccacttt ggcaccccag gctatggaag cccccacttc
     4981 accacactct actcagtccc ttttcctgag ggcgaggcct ttccctctgt tcccgtcact
     5041 gctctgggct ctcccatgca ttcaaacgct agcggcagcg gcgccacaaa cttctctctg
     5101 ctaaagcaag caggtgatgt tgaagaaaac cccgggcctg catgcatgta taacatgatg
     5161 gagacggagc tgaagccgcc gggcccgcag caagcttcgg ggggcggcgg cggaggaggc
     5221 aacgccacgg cggcggcgac cggcggcaac cagaagaaca gcccggaccg cgtcaagagg
     5281 cccatgaacg ccttcatggt atggtcccgg gggcagcggc gtaagatggc ccaggagaac
     5341 cccaagatgc acaactcgga gatcagcaag cgcctgggcg cggagtggaa acttttgtcc
     5401 gagaccgaga agcggccgtt catcgacgag gccaagcggc tgcgcgctct gcacatgaag
     5461 gagcacccgg attataaata ccggccgcgg cggaaaacca agacgctcat gaagaaggat
     5521 aagtacacgc ttcccggagg cttgctggcc cccggcggga acagcatggc gagcggggtt
     5581 ggggtgggcg ccggcctggg tgcgggcgtg aaccagcgca tggacagcta cgcgcacatg
     5641 aacggctgga gcaacggcag ctacagcatg atgcaggagc agctgggcta cccgcagcac
     5701 ccgggcctca acgctcacgg cgcggcacag atgcaaccga tgcaccgcta cgacgtcagc
     5761 gccctgcagt acaactccat gaccagctcg cagacctaca tgaacggctc gcccacctac
     5821 agcatgtcct actcgcagca gggcaccccc ggtatggcgc tgggctccat gggctctgtg
     5881 gtcaagtccg aggccagctc cagccccccc gtggttacct cttcctccca ctccagggcg
     5941 ccctgccagg ccggggacct ccgggacatg atcagcatgt acctccccgg cgccgaggtg
     6001 ccggagcccg ctgcgcccag tagactgcac atggcccagc actaccagag cggcccggtg
     6061 cccggcacgg ccattaacgg cacactgccc ctgtcgcaca tggcatgcgg ctccggcgag
     6121 ggcaggggaa gtcttctaac atgcggggac gtggaggaaa atcccggccc actcgagatg
     6181 aggcagccac ctggcgagtc tgacatggct gtcagcgacg ctctgctccc gtccttctcc
     6241 acgttcgcgt ccggcccggc gggaagggag aagacactgc gtccagcagg tgccccgact
     6301 aaccgttggc gtgaggaact ctctcacatg aagcgacttc ccccacttcc cggccgcccc
     6361 tacgacctgg cggcgacggt ggccacagac ctggagagtg gcggagctgg tgcagcttgc
     6421 agcagtaaca acccggccct cctagcccgg agggagaccg aggagttcaa cgacctcctg
     6481 gacctagact ttatcctttc caactcgcta acccaccagg aatcggtggc cgccaccgtg
     6541 accacctcgg cgtcagcttc atcctcgtct tccccagcga gcagcggccc tgccagcgcg
     6601 ccctccacct gcagcttcag ctatccgatc cgggccgggg gtgacccggg cgtggctgcc
     6661 agcaacacag gtggagggct cctctacagc cgagaatctg cgccacctcc cacggccccc
     6721 ttcaacctgg cggacatcaa tgacgtgagc ccctcgggcg gcttcgtggc tgagctcctg
     6781 cggccggagt tggacccagt atacattccg ccacagcagc ctcagccgcc aggtggcggg
     6841 ctgatgggca agtttgtgct gaaggcgtct ctgaccaccc ctggcagcga gtacagcagc
     6901 ccttcggtca tcagtgttag caaaggaagc ccagacggca gccaccccgt ggtagtggcg
     6961 ccctacagcg gtggcccgcc gcgcatgtgc cccaagatta agcaagaggc ggtcccgtcc
     7021 tgcacggtca gccggtccct agaggcccat ttgagcgctg gaccccagct cagcaacggc
     7081 caccggccca acacacacga cttccccctg gggcggcagc tccccaccag gactacccct
     7141 acactgagtc ccgaggaact gctgaacagc agggactgtc accctggcct gcctcttccc
     7201 ccaggattcc atccccatcc ggggcccaac taccctcctt tcctgccaga ccagatgcag
     7261 tcacaagtcc cctctctcca ttatcaagag ctcatgccac cgggttcctg cctgccagag
     7321 gagcccaagc caaagagggg aagaaggtcg tggccccgga aaagaacagc cacccacact
     7381 tgtgactatg caggctgtgg caaaacctat accaagagtt ctcatctcaa ggcacacctg
     7441 cgaactcaca caggcgagaa accttaccac tgtgactggg acggctgtgg gtggaaattc
     7501 gcccgctccg atgaactgac caggcactac cgcaaacaca cagggcaccg gccctttcag
     7561 tgccagaagt gcgacagggc cttttccagg tcggaccacc ttgccttaca catgaagagg
     7621 cactttctcg agggctcggg ccagtgtact aattatgctc tcttgaaatt ggctggagat
     7681 gttgagagca acccaggtcc cggcgcgcca ctggatttcc tttgggcgtt ggaaaccccg
     7741 cagacagcca cgacgatgcc cctcaacgtg aacttcacca acaggaacta tgacctcgac
     7801 tacgactccg tacagcccta tttcatctgc gacgaggaag agaatttcta tcaccagcaa
     7861 cagcagagcg agctgcagcc gcccgcgccc agtgaggata tctggaagaa attcgagctg
     7921 cttcccaccc cgcccctgtc cccgagccgc cgctccgggc tctgctctcc atcctatgtt
     7981 gcggtcgcta cgtccttctc cccaagggaa gacgatgacg gcggcggtgg caacttctcc
     8041 accgccgatc agctggagat gatgaccgag ttacttggag gagacatggt gaaccagagc
     8101 ttcatctgcg atcctgacga cgagaccttc atcaagaaca tcatcatcca ggactgtatg
     8161 tggagcggtt tctcagccgc tgccaagctg gtctcggaga agctggcctc ctaccaggct
     8221 gcgcgcaaag acagcaccag cctgagcccc gcccgcgggc acagcgtctg ctccacctcc
     8281 agcctgtacc tgcaggacct caccgccgcc gcgtccgagt gcattgaccc ctcagtggtc
     8341 tttccctacc cgctcaacga cagcagctcg cccaaatcct gtacctcgtc cgattccacg
     8401 gccttctctc cttcctcgga ctcgctgctg tcctccgagt cctccccacg ggccagccct
     8461 gagcccctag tgctgcatga ggagacaccg cccaccacca gcagcgactc tgaagaagag
     8521 caagaagatg aggaagaaat tgatgtggtg tctgtggaga agaggcaaac ccctgccaag
     8581 aggtcggagt cgggctcatc tccatcccga ggccacagca aacctccgca cagcccactg
     8641 gtcctcaaga ggtgccacgt ctccactcac cagcacaact acgccgcacc cccctccaca
     8701 aggaaggact atccagctgc caagagggcc aagttggaca gtggcagggt cctgaagcag
     8761 atcagcaaca accgcaagtg ctccagcccc aggtcctcag acacggagga aaacgacaag
     8821 aggcggacac acaacgtctt ggaacgtcag aggaggaacg agctgaagcg cagctttttt
     8881 gccctgcgtg accagatccc tgaattggaa aacaacgaaa aggcccccaa ggtagtgatc
     8941 ctcaaaaaag ccaccgccta catcctgtcc attcaagcag acgagcacaa gctcacctct
     9001 gaaaaggact tattgaggaa acgacgagaa cagttgaaac acaaactcga acagcttcga
     9061 aactctggtg cataaggcgc gccgaattcg tcgagggacc taataacttc gtatagcata
     9121 cattatacga agttatacat gtttaagggt tccggttcca ctaggtacaa ttcgatatca
     9181 agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     9241 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     9301 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     9361 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     9421 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     9481 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     9541 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     9601 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     9661 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     9721 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     9781 atcgataccg tcgacctcga tcgagaccta gaaaaacatg gagcaatcac aagtagcaat
     9841 acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt
     9901 tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     9961 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
    10021 gatatccttg atctgtggat ctaccacaca caaggctact tccctgattg gcagaactac
    10081 acaccagggc cagggatcag atatccactg acctttggat ggtgctacaa gctagtacca
    10141 gttgagcaag agaaggtaga agaagccaat gaaggagaga acacccgctt gttacaccct
    10201 gtgagcctgc atgggatgga tgacccggag agagaagtat tagagtggag gtttgacagc
    10261 cgcctagcat ttcatcacat ggcccgagag ctgcatccgg actgtactgg gtctctctgg
    10321 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
    10381 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
    10441 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag catgtgagca
    10501 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
    10561 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
    10621 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
    10681 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
    10741 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
    10801 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
    10861 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
    10921 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
    10981 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
    11041 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
    11101 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
    11161 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
    11221 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
    11281 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
    11341 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
    11401 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
    11461 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
    11521 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
    11581 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
    11641 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
    11701 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
    11761 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
    11821 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
    11881 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
    11941 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
    12001 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
    12061 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
    12121 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
    12181 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    12241 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
    12301 gac
//
