LOCUS       sequence-95562-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressed nuclear-localized GCaMP6-slow, a calcium indicator, in 
            neurons in zebrafish larva. The plasmid is in Tol2 transposon 
            backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Freeman J, Vladimirov N, Kawashima T, Mu Y, Sofroniew NJ, Bennett 
            DV, Rosen J, Yang CT, Looger LL, Ahrens MB
  TITLE     Mapping brain activity at scale with cluster computing.
  JOURNAL   Nat Methods. 2014 Jul 27. doi: 10.1038/nmeth.3041.
  PUBMED    25068736
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Jul 27. doi: 10.1038/nmeth.3041."
COMMENT     Sequence Label: Tol2-elavl3-H2B-GCaMP6s
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      62..71
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(join(78..92,93..101))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             complement(105..137)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(141..158)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     regulatory      164..173
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(186..563)
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
ORIGIN
        1 agccgaccta tagctctgac tgcgtgacct gtcttattcc acttacgacg tgatgagtcg
       61 accatggtgg cgagatcctt atcgtcatcg tcgtacagat cccgacccat ttgctgtcca
      121 ccagtcatgc tagccatacc atgatgatga tgatgatgag aacccatggt ggcgactggt
      181 ggatccttag cgctggtgta cttggtgatg gccttagtac cctcggacac ggcgtgcttg
      241 gccaactccc caggcagcag caggcgcacg gccgtctgga tctccctgga ggtgatggtc
      301 gagcgcttgt tgtaatgcgc caggcgggaa gcctcacctg cgatgcgctc gaaaatgtcg
      361 ttcacaaacg aattcatgat gcccatggcc ttggacgaaa tgccggtgtc agggtggacc
      421 tgcttcagaa ccttgtacac atagatggaa tagctctcct tgcggctgcg cttgcgcttc
      481 ttgccgcctt tcttctgcgc cttagtcacc gccttcttgg agcccttttt cggggcggga
      541 gcagacttcg ctggctctgg cataccggta attatctgca ggtggaaaat ataacaggtt
      601 gggtgaggaa ccataatttg aggggtgtaa tgcttggcat tctcgctgag aggttaaaaa
      661 tcaatgcgtc tgagagggat agggcctgag gaggacaata tcccaccctt cctcctcctc
      721 tgacctccct cacttcagct ctctccatca gcccctggcc ctgcagatgt cacagtcctt
      781 tcactcattc ccaacccccc
//
