LOCUS       sequence-95570-e         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressed GCaMP6-slow, a calcium indicator, in neurons in zebrafish 
            larva. The plasmid is in Tol2 transposon backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Vladimirov N, Mu Y, Kawashima T, Bennett DV, Yang CT, Looger LL, 
            Keller PJ, Freeman J, Ahrens MB
  TITLE     Light-sheet functional imaging in fictively behaving zebrafish.
  JOURNAL   Nat Methods. 2014 Jul 27. doi: 10.1038/nmeth.3040.
  PUBMED    25068735
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Jul 27. doi: 10.1038/nmeth.3040."
COMMENT     Sequence Label: Tol2-elavl3-GCaMP6s
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      65..74
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(join(81..95,96..104))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             complement(108..140)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(144..161)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctcagccgac ctatagctct gactgcgtga cctgtcttat tccacttacg acgtgatgag
       61 tcgaccatgg tggcgagatc cttatcgtca tcgtcgtaca gatcccgacc catttgctgt
      121 ccaccagtca tgctagccat accatgatga tgatgatgat gagaacccat accggtaatt
      181 atctgcaggt ggaaaatata acaggttggg tgaggaacca taatttgagg ggtgtaatgc
      241 ttggcattct cgctgagagg ttaaaaatca atgcgtctga gagggatagg gcctgaggag
      301 gacaatatcc cacccttcct cctcctctga cctccctcac ttcagctctc tccatcagcc
      361 cctggccctg cagatgtcac agtcctttca ctcattccca accccccctt tacgctgtcc
      421 agggtcaaac tgtgttacgt cactcgccgc tgatgacaaa gggaggacga aaaagcatag
      481 ctagcataac taaaccttgt ttatgtgaaa agtgtgtgcg tttgtgtctg ggttttcggt
      541 tcatccgtgt tgctgtggag cacagcgtga tgcttaattt ggcctacagg ggattttcaa
      601 agaacagatg tatgcaggtt gtagatgcta ctttcagttt ttaacggtcg ctttctgtca
      661 caatgcacaa gttctcagag caagcactct gcatcgtacc agaagggatt tgccaatttt
      721 atctatgttg atttattttt ttcgatgttt tcccagggct ggtaactatc cagaatgtac
      781 a
//
