LOCUS       sequence-95574-e         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP(14) Kozak 
            mKate-G8-L7Ae-PEST 4xFF4 attB2 SGP2(98/30) XbaI attB1 EBFP2 ochre 
            attB2 (insert) AscI (truncated E1) 3'UTR Poly A I-SceI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Wroblewska L, Kitada T, Endo K, Siciliano V, Stillo B, Saito H, 
            Weiss R
  TITLE     Mammalian synthetic circuits with RNA binding proteins for RNA-only 
            delivery.
  JOURNAL   Nat Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301.
  PUBMED    26237515
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301."
COMMENT     Sequence Label: pTK331
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cgactcacta tagatgggcg gcgcatgaga gaagcccaga ccaattacct acccaaaatg
       61 gagaaagttc acgttgacat cgaggaagac agcccattcc tcagagcttt gcagcggagc
      121 ttcccgcagt ttgaggtaga agccaagcag gtcactgata atgaccatgc taatgccaga
      181 gcgttttcgc atctggcttc aaaactgatc gaaacggagg tggacccatc cgacacgatc
      241 cttgacattg gaagtgcgcc cgcccgcaga atgtattcta agcacaagta tcattgtatc
      301 tgtccgatga gatgtgcgga agatccggac agattgtata agtatgcaac taagctgaag
      361 aaaaactgta aggaaataac tgataaggaa ttggacaaga aaatgaagga gctcgccgcc
      421 gtcatgagcg accctgacct ggaaactgag actatgtgcc tccacgacga cgagtcgtgt
      481 cgctacgaag ggcaagtcgc tgtttaccag gatgtatacg cggttgacgg accgacaagt
      541 ctctatcacc aagccaataa gggagttaga gtcgcctact ggataggctt tgacaccacc
      601 ccttttatgt ttaagaactt ggctggagca tatccatcat actctaccaa ctgggccgac
      661 gaaaccgtgt taacggctcg taacataggc ctatgcagct ctgacgttat ggagcggtca
      721 cgtagaggga tgtccattct tagaaagaag tatttgaaac catccaacaa tgttctattc
      781 tctgttggct cgaccatcta ccacgagaag agggacttac tgaggagctg gcacctgccg
      841 tctgtatttc acttacgtgg caagcaaaat tacacatgtc ggtgtgagac tatagttagt
      901 tgcgacgggt acgtcg
//
