LOCUS       sequence-95576-e         588 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cas9 nuclease and gRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Sakuma T, Nishikawa A, Kume S, Chayama K, Yamamoto T
  TITLE     Multiplex genome engineering in human cells using all-in-one 
            CRISPR/Cas9 vector system.
  JOURNAL   Sci Rep. 2014 Jun 23;4:5400. doi: 10.1038/srep05400.
  PUBMED    24954249
REFERENCE   2  (bases 1 to 588)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2014-06-23"; volume: "4:5400. doi"; pages: " 
            10.1038/srep05400"
COMMENT     Sequence Label: pX330S-2
FEATURES             Location/Qualifiers
     source          1..588
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(321..341)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(348..413)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     regulatory      413..422
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctgtggaaga agctgtcgtc caccttggcc atctcgttgc tgaagatctc ttgcagatag
       61 cagatccggt tcttccgtct ggtgtatctt cttctggcgg ttctcttcag ccgggtggcc
      121 tcggctgttt cgccgctgtc gaacagcagg gctccgatca ggttcttctt gatgctgtgc
      181 cggtcggtgt tgcccagcac cttgaatttc ttgctgggca ccttgtactc gtcggtgatc
      241 acggcccagc ccacagagtt ggtgccgatg tccaggccga tgctgtactt cttgtcggct
      301 gctgggactc cgtggatacc gaccttccgc ttcttctttg gggccatctt atcgtcatcg
      361 tctttgtaat caatatcatg atccttgtag tctccgtcgt ggtccttata gtccatggtg
      421 gcaccggtcc aacctgaaaa aaagtgattt caggcaggtg ctccaggtaa ttaaacatta
      481 ataccccacc aaccaaccat cccttaaacc cttacctctt gctcagctaa ttacagcccg
      541 gaggagaagg gccgtcccgc ccgctcacct gtgggagtaa cgcggtca
//
