LOCUS       sequence-95577-e         695 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for use as a standard for titering experiments 
            when trying to compare vectors with different selection markers or 
            to determine appropriate antibiotic conditions..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 695)
  TITLE     David Root plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 695)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pRosetta
FEATURES             Location/Qualifiers
     source          1..695
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(36..55)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     532..552
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 cgaatcaccg acctctctcc ccagggggat ccaccatgac cgagtacaag cccaccgtgc
       61 gcctcgccac ccgcgacgac gtgcccaggg ccgtccgcac cctcgccgcc gctttcgccg
      121 actaccccgc cacccgccac accgtcgatc ccgaccgcca catcgagcgg gtcaccgagc
      181 tgcaagaact cttcctcacc cgcgtcgggc tcgacatcgg caaggtgtgg gtcgctgacg
      241 acggcgccgc agtggctgtc tggaccacac ccgagagcgt cgaagctggg gcggtgttcg
      301 ccgagatcgg ccctcgcatg gccgagttga gcggttcccg gctggccgct cagcaacaga
      361 tggaaggcct cctggctcca caccggccca aggagcccgc atggttcctg gccaccgtcg
      421 gcgtctctcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
      481 aggctgccga gcgcgccggg gtgcccgcct tcctggagac ctccgcaccc cgcaacctcc
      541 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt ggaggtgccc gaaggaccac
      601 gcacctggtg catgacccgc aagcccggtg ccctgggaag cggagctact aacttcagcc
      661 tgctgaagca ggctggagac gtggaggaga accct
//
