LOCUS       sequence-95582-e         642 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cas9 nuclease and gRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Sakuma T, Nishikawa A, Kume S, Chayama K, Yamamoto T
  TITLE     Multiplex genome engineering in human cells using all-in-one 
            CRISPR/Cas9 vector system.
  JOURNAL   Sci Rep. 2014 Jun 23;4:5400. doi: 10.1038/srep05400.
  PUBMED    24954249
REFERENCE   2  (bases 1 to 642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2014-06-23"; volume: "4:5400. doi"; pages: " 
            10.1038/srep05400"
COMMENT     Sequence Label: pX330S-4
FEATURES             Location/Qualifiers
     source          1..642
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        100..348
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     100..120
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     271..290
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        367..442
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
       61 ttttgctggc cttttgctca ggtctcacta gaggcatgtg agggcctatt tcccatgatt
      121 ccttcatatt tgcatatacg atacaaggct gttagagaga taattggaat taatttgact
      181 gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt tcttgggtag
      241 tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt aacttgaaag
      301 tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccgg gtcttcgaga
      361 agacctgttt tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga
      421 aaaagtggca ccgagtcggt gcttttttgt tttagagcta gaaatagcaa gttaaaataa
      481 ggctagtccg tttttagcgc gtgcgccaat tctgcagaca aatggctcta gatgagaccc
      541 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
      601 acgtcaatag taacgccaat agggactttc cattgacgtc aa
//
