LOCUS       sequence-95593-e         495 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cas9 nuclease and gRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Sakuma T, Nishikawa A, Kume S, Chayama K, Yamamoto T
  TITLE     Multiplex genome engineering in human cells using all-in-one 
            CRISPR/Cas9 vector system.
  JOURNAL   Sci Rep. 2014 Jun 23;4:5400. doi: 10.1038/srep05400.
  PUBMED    24954249
REFERENCE   2  (bases 1 to 495)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2014-06-23"; volume: "4:5400. doi"; pages: " 
            10.1038/srep05400"
COMMENT     Sequence Label: pX330S-6
FEATURES             Location/Qualifiers
     source          1..495
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        126..374
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     126..146
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     297..316
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        393..468
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
       61 cnacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcagg tctcaagagg
      121 catgtgaggg cctatttccc atgattcctt catatttgca tatacgatac aaggctgtta
      181 gagagataat tggaattaat ttgactgtaa acacaaagat attagtacaa aatacgtgac
      241 gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact
      301 atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
      361 aaggacgaaa caccgggtct tcgagaagac ctgttttaga gctagaaata gcaagttaaa
      421 ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt ttttgtttta
      481 gagctagaaa tagca
//
