LOCUS       sequence-9560-ex        9814 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9814)
  AUTHORS   Brambrink T, Foreman R, Welstead GG, Lengner CJ, Wernig M, Suh H, 
            Jaenisch R
  TITLE     Sequential expression of pluripotency markers during direct 
            reprogramming of mouse somatic cells.
  JOURNAL   Cell Stem Cell. 2008 Feb 7. 2(2):151-9.
  PUBMED    18371436
REFERENCE   2  (bases 1 to 9814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2008 Feb 7. 2(2):151-9."
COMMENT     Sequence Label: TetO-FUW-cMYC
FEATURES             Location/Qualifiers
     source          1..9814
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        388..586
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             604..784
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    831..956
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1449..1682
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1867..1911
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2209..2326
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2383..2401,2402..2424,2425..2443,2444..2466,2467..2485,
                     2486..2508,2509..2527,2528..2550,2551..2569,2570..2592,
                     2593..2611,2612..2634,2635..2653)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2686..2723
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2720..2744
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    4275..4863
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4328..4348)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4746..4757)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4866..4882)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4867..4883)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5388..5568
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5594..5611)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5600..5824
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5870..6298
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5957..5976)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6167..6188
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6307..6327)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6312..6641
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6492..6627
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6554..6573
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6689..6736
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6755..7129
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7259..7380
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7296..7315)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7350..7369
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7429..7445)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7429..7445)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7442..7464)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7453..7469
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7477..7483,7484..7501,7502..7507))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7522..7543
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7660..7677)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7831..8419)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7911..7930)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8590..9381,9382..9450))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9213..9232
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9451..9555)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9630..9649)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
      601 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
      661 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
      721 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
      781 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
      841 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
      901 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
      961 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     1021 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     1081 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     1141 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     1201 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     1261 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
     1321 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     1381 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     1441 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     1501 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     1561 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     1621 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     1681 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     1741 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     1801 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     1861 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     1921 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     1981 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     2041 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
     2101 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg
     2161 gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa
     2221 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     2281 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     2341 ggacagcaga gatccagttt ggttagatct cgagtttacc actccctatc agtgatagag
     2401 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
     2461 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
     2521 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     2581 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     2641 tcagtgatag agaaaagtga aagtcgagct cggtacccgg gtcgagtagg cgtgtacggt
     2701 gggaggccta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc
     2761 cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgc ggccccgaat
     2821 tcgcccttag agctcctcga gctgtttgaa ggctggattt cctttgggcg ttggaaaccc
     2881 cgcagacagc cacgacgatg cccctcaacg tgaacttcac caacaggaac tatgacctcg
     2941 actacgactc cgtacagccc tatttcatct gcgacgagga agagaatttc tatcaccagc
     3001 aacagcagag cgagctgcag ccgcccgcgc ccagtgagga tatctggaag aaattcgagc
     3061 tgcttcccac cccgcccctg tccccgagcc gccgctccgg gctctgctct ccatcctatg
     3121 ttgcggtcgc tacgtccttc tccccaaggg aagacgatga cggcggcggt ggcaacttct
     3181 ccaccgccga tcagctggag atgatgaccg agttacttgg aggagacatg gtgaaccaga
     3241 gcttcatctg cgatcctgac gacgagacct tcatcaagaa catcatcatc caggactgta
     3301 tgtggagcgg tttctcagcc gctgccaagc tggtctcgga gaagctggcc tcctaccagg
     3361 ctgcgcgcaa agacagcacc agcctgagcc ccgcccgcgg gcacagcgtc tgctccacct
     3421 ccagcctgta cctgcaggac ctcaccgccg ccgcgtccga gtgcattgac ccctcagtgg
     3481 tctttcccta cccgctcaac gacagcagct cgcccaaatc ctgtacctcg tccgattcca
     3541 cggccttctc tccttcctcg gactcgctgc tgtcctccga gtcctcccca cgggccagcc
     3601 ctgagcccct agtgctgcat gaggagacac cgcccaccac cagcagcgac tctgaagaag
     3661 agcaagaaga tgaggaagaa attgatgtgg tgtctgtgga gaagaggcaa acccctgcca
     3721 agaggtcgga gtcgggctca tctccatccc gaggccacag caaacctccg cacagcccac
     3781 tggtcctcaa gaggtgccac gtctccactc accagcacaa ctacgccgca cccccctcca
     3841 caaggaagga ctatccagct gccaagaggg ccaagttgga cagtggcagg gtcctgaagc
     3901 agatcagcaa caaccgcaag tgctccagcc ccaggtcctc agacacggag gaaaacgaca
     3961 agaggcggac acacaacgtc ttggaacgtc agaggaggaa cgagctgaag cgcagctttt
     4021 ttgccctgcg tgaccagatc cctgaattgg aaaacaacga aaaggccccc aaggtagtga
     4081 tcctcaaaaa agccaccgcc tacatcctgt ccattcaagc agacgagcac aagctcacct
     4141 ctgaaaagga cttattgagg aaacgacgag aacagttgaa acacaaactc gaacagcttc
     4201 gaaactctgg tgcataaact gacctaactc gaggaggagc tggaaagggc gaattcgata
     4261 tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     4321 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     4381 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     4441 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     4501 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     4561 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     4621 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     4681 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     4741 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     4801 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     4861 cgcatcgata ccgtcgacct cgagacctag aaaaacatgg agcaatcaca agtagcaata
     4921 cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt
     4981 ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct gtagatctta
     5041 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     5101 atatccttga tctgtggatc taccacacac aaggctactt ccctgattgg cagaactaca
     5161 caccagggcc agggatcaga tatccactga cctttggatg gtgctacaag ctagtaccag
     5221 ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa cacccgcttg ttacaccctg
     5281 tgagcctgca tgggatggat gacccggaga gagaagtatt agagtggagg tttgacagcc
     5341 gcctagcatt tcatcacatg gcccgagagc tgcatccgga ctgtactggg tctctctggt
     5401 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     5461 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     5521 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa
     5581 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     5641 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     5701 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     5761 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     5821 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
     5881 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     5941 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     6001 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     6061 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     6121 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     6181 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     6241 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
     6301 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     6361 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     6421 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     6481 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     6541 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
     6601 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
     6661 tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat agtatatcgg
     6721 catagtataa tacgacaagg tgaggaacta aaccatggcc aagttgacca gtgccgttcc
     6781 ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt
     6841 ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt
     6901 catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg
     6961 cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc
     7021 ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg
     7081 cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgac acgtgctacg
     7141 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     7201 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
     7261 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     7321 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     7381 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     7441 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     7501 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     7561 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     7621 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     7681 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     7741 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     7801 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     7861 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     7921 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     7981 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     8041 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     8101 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     8161 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     8221 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     8281 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     8341 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     8401 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     8461 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     8521 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     8581 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     8641 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     8701 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     8761 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     8821 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     8881 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     8941 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     9001 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     9061 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     9121 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     9181 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     9241 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     9301 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     9361 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     9421 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     9481 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     9541 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga
     9601 tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     9661 cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa
     9721 gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt
     9781 tttgcgctgc ttcgcgatgt acgggccaga tata
//
