LOCUS       sequence-95609-e         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP XbaI attB1 Kozak 
            mKate opal attB2 AscI (truncated E1) 3'UTR Poly A I-SceI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Wroblewska L, Kitada T, Endo K, Siciliano V, Stillo B, Saito H, 
            Weiss R
  TITLE     Mammalian synthetic circuits with RNA binding proteins for RNA-only 
            delivery.
  JOURNAL   Nat Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301.
  PUBMED    26237515
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301."
COMMENT     Sequence Label: pTK194
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gatgggcggc gcatgagaga agcccagacc aattacctac ccaaaatgga gaaagttcac
       61 gttgacatcg aggaagacag cccattcctc agagctttgc agcggagctt cccgcagttt
      121 gaggtagaag ccaagcaggt cactgataat gaccatgcta atgccagagc gttttcgcat
      181 ctggcttcaa aactgatcga aacggaggtg gacccatccg acacgatcct tgacattgga
      241 agtgcgcccg cccgcagaat gtattctaag cacaagtatc attgtatctg tccgatgaga
      301 tgtgcggaag atccggacag attgtataag tatgcaacta agctgaagaa aaactgtaag
      361 gaaataactg ataaggaatt ggacaagaaa atgaaggagc tcgccgccgt catgagcgac
      421 cctgacctgg aaactgagac tatgtgcctc cacgacgacg agtcgtgtcg ctacgaaggg
      481 caagtcgctg tttaccagga tgtatacgcg gttgacggac cgacaagtct ctatcaccaa
      541 gccaataagg gagttagagt cgcctactgg ataggctttg acaccacccc ttttatgttt
      601 aagaacttgg ctggagcata tccatcatac tctaccaact gggccgacga aaccgtgtta
      661 acggctcgta acataggcct atgcagctct gacgttatgg agcggtcacg tagagggatg
      721 tccattctta gaaagaagta tttgaaacca tccaacaatg ttctattctc tgttggctcg
      781 accatctacc acgagaagag ggacttactg aggagctggc acctg
//
