LOCUS       sequence-9561-ex       12306 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid expressing mouse Sox2, Oct4, Klf4 and cMyc for 
            iPS cell generation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12306)
  AUTHORS   Carey BW, Markoulaki S, Hanna J, Saha K, Gao Q, Mitalipova M, 
            Jaenisch R
  TITLE     Reprogramming of murine and human somatic cells using a single 
            polycistronic vector.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Jan 6. 106(1):157-62.
  PUBMED    19109433
REFERENCE   2  (bases 1 to 12306)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2009 Jan 6. 106(1):157-62."
COMMENT     Sequence Label: FUW-SOKM
FEATURES             Location/Qualifiers
     source          1..12306
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1686..1919
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2104..2148
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2446..2563
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     2622..2638
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2680..2713)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2763..3974
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3811..3830
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             4987..5043
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6121..6174
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7645..7704
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     protein_bind    complement(9106..9139)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    9195..9783
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9248..9268)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9666..9677)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9786..9802)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             10312..10492
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(10554..11142)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10634..10653)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11313..12104,12105..12173))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11936..11955
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12174..12278)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg gccgcgctga tcttcagacc tggaggagga
     1561 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     1621 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     1681 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     1741 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     1801 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1861 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1921 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1981 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     2041 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     2101 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     2161 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     2221 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     2281 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     2341 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca
     2401 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg
     2461 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2521 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2581 cagcagagat ccagtttggt tagtaccggg cccgctctag tcgaggtcga cggtatcgat
     2641 aagctcgctt cacgagattc cagcaggtcg agggacctaa taacttcgta tagcatacat
     2701 tatacgaagt tatattaagg gttccaagct taagcggcct aacccgtgtc ggctccagat
     2761 ctggcctccg cgccgggttt tggcgcctcc cgcgggcgcc cccctcctca cggcgagcgc
     2821 tgccacgtca gacgaagggc gcagcgagcg tcctgatcct tccgcccgga cgctcaggac
     2881 agcggcccgc tgctcataag actcggcctt agaaccccag tatcagcaga aggacatttt
     2941 aggacgggac ttgggtgact ctagggcact ggttttcttt ccagagagcg gaacaggcga
     3001 ggaaaagtag tcccttctcg gcgattctgc ggagggatct ccgtggggcg gtgaacgccg
     3061 atgattatat aaggacgcgc cgggtgtggc acagctagtt ccgtcgcagc cgggatttgg
     3121 gtcgcggttc ttgtttgtgg atcgctgtga tcgtcacttg gtgagtagcg ggctgctggg
     3181 ctggccgggg ctttcgtggc cgccgggccg ctcggtggga cggaagcgtg tggagagacc
     3241 gccaagggct gtagtctggg tccgcgagca aggttgccct gaactggggg ttggggggag
     3301 cgcagcaaaa tggcggctgt tcccgagtct tgaatggaag acgcttgtga ggcgggctgt
     3361 gaggtcgttg aaacaaggtg gggggcatgg tgggcggcaa gaacccaagg tcttgaggcc
     3421 ttcgctaatg cgggaaagct cttattcggg tgagatgggc tggggcacca tctggggacc
     3481 ctgacgtgaa gtttgtcact gactggagaa ctcggtttgt cgtctgttgc gggggcggca
     3541 gttatggcgg tgccgttggg cagtgcaccc gtacctttgg gagcgcgcgc cctcgtcgtg
     3601 tcgtgacgtc acccgttctg ttggcttata atgcagggtg gggccacctg ccggtaggtg
     3661 tgcggtaggc ttttctccgt cgcaggacgc agggttcggg cctagggtag gctctcctga
     3721 atcgacaggc gccggacctc tggtgagggg agggataagt gaggcgtcag tttctttggt
     3781 cggttttatg tacctatctt cttaagtagc tgaagctccg gttttgaact atgcgctcgg
     3841 ggttggcgag tgtgttttgt gaagtttttt aggcaccttt tgaaatgtaa tcatttgggt
     3901 caatatgtaa ttttcagtgt tagactagta aattgtccgc taaattctgg ccgtttttgg
     3961 cttttttgtt agacgaagct tgggctgcag gtcgactcta gagaattcgc caccatgtat
     4021 aacatgatgg agacggagct gaagccgccg ggcccgcagc aagcttcggg gggcggcggc
     4081 ggaggaggca acgccacggc ggcggcgacc ggcggcaacc agaagaacag cccggaccgc
     4141 gtcaagaggc ccatgaacgc cttcatggta tggtcccggg ggcagcggcg taagatggcc
     4201 caggagaacc ccaagatgca caactcggag atcagcaagc gcctgggcgc ggagtggaaa
     4261 cttttgtccg agaccgagaa gcggccgttc atcgacgagg ccaagcggct gcgcgctctg
     4321 cacatgaagg agcacccgga ttataaatac cggccgcggc ggaaaaccaa gacgctcatg
     4381 aagaaggata agtacacgct tcccggaggc ttgctggccc ccggcgggaa cagcatggcg
     4441 agcggggttg gggtgggcgc cggcctgggt gcgggcgtga accagcgcat ggacagctac
     4501 gcgcacatga acggctggag caacggcagc tacagcatga tgcaggagca gctgggctac
     4561 ccgcagcacc cgggcctcaa cgctcacggc gcggcacaga tgcaaccgat gcaccgctac
     4621 gacgtcagcg ccctgcagta caactccatg accagctcgc agacctacat gaacggctcg
     4681 cccacctaca gcatgtccta ctcgcagcag ggcacccccg gtatggcgct gggctccatg
     4741 ggctctgtgg tcaagtccga ggccagctcc agcccccccg tggttacctc ttcctcccac
     4801 tccagggcgc cctgccaggc cggggacctc cgggacatga tcagcatgta cctccccggc
     4861 gccgaggtgc cggagcccgc tgcgcccagt agactgcaca tggcccagca ctaccagagc
     4921 ggcccggtgc ccggcacggc cattaacggc acactgcccc tgtcgcacat ggctagcggc
     4981 agcggcgcca caaacttctc tctgctaaag caagcaggtg atgttgaaga aaaccccggg
     5041 cctgcatgca tggctggaca cctggcttca gacttcgcct tctcaccccc accaggtggg
     5101 ggtgatgggt cagcagggct ggagccgggc tgggtggatc ctcgaacctg gctaagcttc
     5161 caagggcctc caggtgggcc tggaatcgga ccaggctcag aggtattggg gatctcccca
     5221 tgtccgcccg catacgagtt ctgcggaggg atggcatact gtggacctca ggttggactg
     5281 ggcctagtcc cccaagttgg cgtggagact ttgcagcctg agggccaggc aggagcacga
     5341 gtggaaagca actcagaggg aacctcctct gagccctgtg ccgaccgccc caatgccgtg
     5401 aagttggaga aggtggaacc aactcccgag gagtcccagg acatgaaagc cctgcagaag
     5461 gagctagaac agtttgccaa gctgctgaag cagaagagga tcaccttggg gtacacccag
     5521 gccgacgtgg ggctcaccct gggcgttctc tttggaaagg tgttcagcca gaccaccatc
     5581 tgtcgcttcg aggccttgca gctcagcctt aagaacatgt gtaagctgcg gcccctgctg
     5641 gagaagtggg tggaggaagc cgacaacaat gagaaccttc aggagatatg caaatcggag
     5701 accctggtgc aggcccggaa gagaaagcga actagcattg agaaccgtgt gaggtggagt
     5761 ctggagacca tgtttctgaa gtgcccgaag ccctccctac agcagatcac tcacatcgcc
     5821 aatcagcttg ggctagagaa ggatgtggtt cgagtatggt tctgtaaccg gcgccagaag
     5881 ggcaaaagat caagtattga gtattcccaa cgagaagagt atgaggctac agggacacct
     5941 ttcccagggg gggctgtatc ctttcctctg cccccaggtc cccactttgg caccccaggc
     6001 tatggaagcc cccacttcac cacactctac tcagtccctt ttcctgaggg cgaggccttt
     6061 ccctctgttc ccgtcactgc tctgggctct cccatgcatt caaacgcatg cggctccggc
     6121 gagggcaggg gaagtcttct aacatgcggg gacgtggagg aaaatcccgg cccactcgag
     6181 atgaggcagc cacctggcga gtctgacatg gctgtcagcg acgctctgct cccgtccttc
     6241 tccacgttcg cgtccggccc ggcgggaagg gagaagacac tgcgtccagc aggtgccccg
     6301 actaaccgtt ggcgtgagga actctctcac atgaagcgac ttcccccact tcccggccgc
     6361 ccctacgacc tggcggcgac ggtggccaca gacctggaga gtggcggagc tggtgcagct
     6421 tgcagcagta acaacccggc cctcctagcc cggagggaga ccgaggagtt caacgacctc
     6481 ctggacctag actttatcct ttccaactcg ctaacccacc aggaatcggt ggccgccacc
     6541 gtgaccacct cggcgtcagc ttcatcctcg tcttccccag cgagcagcgg ccctgccagc
     6601 gcgccctcca cctgcagctt cagctatccg atccgggccg ggggtgaccc gggcgtggct
     6661 gccagcaaca caggtggagg gctcctctac agccgagaat ctgcgccacc tcccacggcc
     6721 cccttcaacc tggcggacat caatgacgtg agcccctcgg gcggcttcgt ggctgagctc
     6781 ctgcggccgg agttggaccc agtatacatt ccgccacagc agcctcagcc gccaggtggc
     6841 gggctgatgg gcaagtttgt gctgaaggcg tctctgacca cccctggcag cgagtacagc
     6901 agcccttcgg tcatcagtgt tagcaaagga agcccagacg gcagccaccc cgtggtagtg
     6961 gcgccctaca gcggtggccc gccgcgcatg tgccccaaga ttaagcaaga ggcggtcccg
     7021 tcctgcacgg tcagccggtc cctagaggcc catttgagcg ctggacccca gctcagcaac
     7081 ggccaccggc ccaacacaca cgacttcccc ctggggcggc agctccccac caggactacc
     7141 cctacactga gtcccgagga actgctgaac agcagggact gtcaccctgg cctgcctctt
     7201 cccccaggat tccatcccca tccggggccc aactaccctc ctttcctgcc agaccagatg
     7261 cagtcacaag tcccctctct ccattatcaa gagctcatgc caccgggttc ctgcctgcca
     7321 gaggagccca agccaaagag gggaagaagg tcgtggcccc ggaaaagaac agccacccac
     7381 acttgtgact atgcaggctg tggcaaaacc tataccaaga gttctcatct caaggcacac
     7441 ctgcgaactc acacaggcga gaaaccttac cactgtgact gggacggctg tgggtggaaa
     7501 ttcgcccgct ccgatgaact gaccaggcac taccgcaaac acacagggca ccggcccttt
     7561 cagtgccaga agtgcgacag ggccttttcc aggtcggacc accttgcctt acacatgaag
     7621 aggcactttc tcgagggctc gggccagtgt actaattatg ctctcttgaa attggctgga
     7681 gatgttgaga gcaacccagg tcccggcgcg ccactggatt tcctttgggc gttggaaacc
     7741 ccgcagacag ccacgacgat gcccctcaac gtgaacttca ccaacaggaa ctatgacctc
     7801 gactacgact ccgtacagcc ctatttcatc tgcgacgagg aagagaattt ctatcaccag
     7861 caacagcaga gcgagctgca gccgcccgcg cccagtgagg atatctggaa gaaattcgag
     7921 ctgcttccca ccccgcccct gtccccgagc cgccgctccg ggctctgctc tccatcctat
     7981 gttgcggtcg ctacgtcctt ctccccaagg gaagacgatg acggcggcgg tggcaacttc
     8041 tccaccgccg atcagctgga gatgatgacc gagttacttg gaggagacat ggtgaaccag
     8101 agcttcatct gcgatcctga cgacgagacc ttcatcaaga acatcatcat ccaggactgt
     8161 atgtggagcg gtttctcagc cgctgccaag ctggtctcgg agaagctggc ctcctaccag
     8221 gctgcgcgca aagacagcac cagcctgagc cccgcccgcg ggcacagcgt ctgctccacc
     8281 tccagcctgt acctgcagga cctcaccgcc gccgcgtccg agtgcattga cccctcagtg
     8341 gtctttccct acccgctcaa cgacagcagc tcgcccaaat cctgtacctc gtccgattcc
     8401 acggccttct ctccttcctc ggactcgctg ctgtcctccg agtcctcccc acgggccagc
     8461 cctgagcccc tagtgctgca tgaggagaca ccgcccacca ccagcagcga ctctgaagaa
     8521 gagcaagaag atgaggaaga aattgatgtg gtgtctgtgg agaagaggca aacccctgcc
     8581 aagaggtcgg agtcgggctc atctccatcc cgaggccaca gcaaacctcc gcacagccca
     8641 ctggtcctca agaggtgcca cgtctccact caccagcaca actacgccgc acccccctcc
     8701 acaaggaagg actatccagc tgccaagagg gccaagttgg acagtggcag ggtcctgaag
     8761 cagatcagca acaaccgcaa gtgctccagc cccaggtcct cagacacgga ggaaaacgac
     8821 aagaggcgga cacacaacgt cttggaacgt cagaggagga acgagctgaa gcgcagcttt
     8881 tttgccctgc gtgaccagat ccctgaattg gaaaacaacg aaaaggcccc caaggtagtg
     8941 atcctcaaaa aagccaccgc ctacatcctg tccattcaag cagacgagca caagctcacc
     9001 tctgaaaagg acttattgag gaaacgacga gaacagttga aacacaaact cgaacagctt
     9061 cgaaactctg gtgcataagg cgcgccgaat tcgtcgaggg acctaataac ttcgtatagc
     9121 atacattata cgaagttata catgtttaag ggttccggtt ccactaggta caattcgata
     9181 tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     9241 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     9301 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     9361 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     9421 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     9481 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     9541 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     9601 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     9661 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     9721 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     9781 cgcatcgata ccgtcgacct cgatcgagac ctagaaaaac atggagcaat cacaagtagc
     9841 aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg
     9901 ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat
     9961 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
    10021 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttggcagaac
    10081 tacacaccag ggccagggat cagatatcca ctgacctttg gatggtgcta caagctagta
    10141 ccagttgagc aagagaaggt agaagaagcc aatgaaggag agaacacccg cttgttacac
    10201 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tattagagtg gaggtttgac
    10261 agccgcctag catttcatca catggcccga gagctgcatc cggactgtac tgggtctctc
    10321 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
    10381 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
    10441 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagcatgtga
    10501 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
    10561 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
    10621 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
    10681 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
    10741 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
    10801 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
    10861 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
    10921 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
    10981 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
    11041 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
    11101 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
    11161 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
    11221 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
    11281 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
    11341 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
    11401 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
    11461 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
    11521 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
    11581 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
    11641 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
    11701 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
    11761 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
    11821 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
    11881 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
    11941 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
    12001 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    12061 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    12121 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    12181 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    12241 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    12301 cctgac
//
