LOCUS       sequence-95618-e         980 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-terminal fusion of EGFP to Drosophila melanogaster Gr21a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Benton R, Sachse S, Michnick SW, Vosshall LB
  TITLE     Atypical membrane topology and heteromeric function of Drosophila 
            odorant receptors in vivo.
  JOURNAL   PLoS Biol. 2006 Feb;4(2):e20. Epub 2006 Jan 17.
  PUBMED    16402857
REFERENCE   2  (bases 1 to 980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2006-02"; volume: "4(2)"; pages: "e20. Epub 2006 Jan 
            17"
COMMENT     Sequence Label: RP156 pUAST EGFP:Gr21a
FEATURES             Location/Qualifiers
     source          1..980
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    61..155
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        174..412
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     359..380
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             423..434
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             join(438..440,441..443,444..956)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(483..504)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(744..763)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 agcttggctg cttccaacgc gttgggagct ctccggatcc aagcttgcat gcctgcaggt
       61 cggagtactg tcctccgagc ggagtactgt cctccgagcg gagtactgtc ctccgagcgg
      121 agtactgtcc tccgagcgga gtactgtcct ccgagcggag actctagcga gcgccggagt
      181 ataaatagag gcgcttcgtc tacggagcga caattcaatt caaacaagca aagtgaacac
      241 gtcgctaagc gaaagctaag caaataaaca agcgcagctg aacaagctaa acaatctgca
      301 gtaaagtgca agttaaagtg aatcaattaa aagtaaccag caaccaagta aatcaactgc
      361 aactactgaa atctgccaag aagtaattat tgaatacaag aagagaactc tgaataggga
      421 attgggaatt gcaaaacatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
      481 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
      541 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
      601 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
      661 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
      721 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
      781 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
      841 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
      901 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
      961 gcagcgtgca gctcgccgac
//
