LOCUS       sequence-9562-ex        9377 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9377)
  AUTHORS   Brambrink T, Foreman R, Welstead GG, Lengner CJ, Wernig M, Suh H, 
            Jaenisch R
  TITLE     Sequential expression of pluripotency markers during direct 
            reprogramming of mouse somatic cells.
  JOURNAL   Cell Stem Cell. 2008 Feb 7. 2(2):151-9.
  PUBMED    18371436
REFERENCE   2  (bases 1 to 9377)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2008 Feb 7. 2(2):151-9."
COMMENT     Sequence Label: TetO-FUW-sox2
FEATURES             Location/Qualifiers
     source          1..9377
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        388..586
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             604..784
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    831..956
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1449..1682
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1867..1911
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2209..2326
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2383..2401,2402..2424,2425..2443,2444..2466,2467..2485,
                     2486..2508,2509..2527,2528..2550,2551..2569,2570..2592,
                     2593..2611,2612..2634,2635..2653)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2686..2723
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2720..2744
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    3838..4426
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3891..3911)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4309..4320)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4429..4445)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4430..4446)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4951..5131
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5157..5174)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5163..5387
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5433..5861
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5520..5539)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5730..5751
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5870..5890)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5875..6204
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6055..6190
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6117..6136
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6252..6299
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6318..6692
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6822..6943
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6859..6878)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6913..6932
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6992..7008)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6992..7008)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7005..7027)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7016..7032
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7040..7046,7047..7064,7065..7070))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7085..7106
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7223..7240)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7394..7982)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7474..7493)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8153..8944,8945..9013))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8776..8795
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9014..9118)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9193..9212)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
      601 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
      661 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
      721 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
      781 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
      841 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
      901 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
      961 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     1021 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     1081 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     1141 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     1201 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     1261 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
     1321 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     1381 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     1441 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     1501 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     1561 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     1621 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     1681 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     1741 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     1801 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     1861 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     1921 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     1981 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     2041 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
     2101 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg
     2161 gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa
     2221 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     2281 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     2341 ggacagcaga gatccagttt ggttagatct cgagtttacc actccctatc agtgatagag
     2401 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
     2461 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
     2521 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     2581 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     2641 tcagtgatag agaaaagtga aagtcgagct cggtacccgg gtcgagtagg cgtgtacggt
     2701 gggaggccta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc
     2761 cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgc ggccccgaat
     2821 tcgcccttca gcgcccgcat gtataacatg atggagacgg agctgaagcc gccgggcccg
     2881 cagcaagctt cggggggcgg cggcggagga ggcaacgcca cggcggcggc gaccggcggc
     2941 aaccagaaga acagcccgga ccgcgtcaag aggcccatga acgccttcat ggtatggtcc
     3001 cgggggcagc ggcgtaagat ggcccaggag aaccccaaga tgcacaactc ggagatcagc
     3061 aagcgcctgg gcgcggagtg gaaacttttg tccgagaccg agaagcggcc gttcatcgac
     3121 gaggccaagc ggctgcgcgc tctgcacatg aaggagcacc cggattataa ataccggccg
     3181 cggcggaaaa ccaagacgct catgaagaag gataagtaca cgcttcccgg aggcttgctg
     3241 gcccccggcg ggaacagcat ggcgagcggg gttggggtgg gcgccggcct gggtgcgggc
     3301 gtgaaccagc gcatggacag ctacgcgcac atgaacggct ggagcaacgg cagctacagc
     3361 atgatgcagg agcagctggg ctacccgcag cacccgggcc tcaacgctca cggcgcggca
     3421 cagatgcaac cgatgcaccg ctacgacgtc agcgccctgc agtacaactc catgaccagc
     3481 tcgcagacct acatgaacgg ctcgcccacc tacagcatgt cctactcgca gcagggcacc
     3541 cccggtatgg cgctgggctc catgggctct gtggtcaagt ccgaggccag ctccagcccc
     3601 cccgtggtta cctcttcctc ccactccagg gcgccctgcc aggccgggga cctccgggac
     3661 atgatcagca tgtacctccc cggcgccgag gtgccggagc ccgctgcgcc cagtagactg
     3721 cacatggccc agcactacca gagcggcccg gtgcccggca cggccattaa cggcacactg
     3781 cccctgtcgc acatgtgagg gctggacaag ggcgaattcg atatcaagct tatcgataat
     3841 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     3901 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     3961 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     4021 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     4081 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     4141 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     4201 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     4261 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     4321 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     4381 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga
     4441 cctcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct
     4501 gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag
     4561 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     4621 aaggggggac tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg
     4681 atctaccaca cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc
     4741 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagca agagaaggta
     4801 gaagaagcca atgaaggaga gaacacccgc ttgttacacc ctgtgagcct gcatgggatg
     4861 gatgacccgg agagagaagt attagagtgg aggtttgaca gccgcctagc atttcatcac
     4921 atggcccgag agctgcatcc ggactgtact gggtctctct ggttagacca gatctgagcc
     4981 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     5041 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     5101 cccttttagt cagtgtggaa aatctctagc agggcccgtt taaacccgct gatcagcctc
     5161 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     5221 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     5281 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     5341 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     5401 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     5461 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     5521 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     5581 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     5641 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     5701 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     5761 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     5821 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
     5881 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     5941 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     6001 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     6061 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     6121 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
     6181 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
     6241 gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca
     6301 aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg
     6361 acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg
     6421 aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg
     6481 accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt
     6541 acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga
     6601 ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact
     6661 gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg
     6721 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     6781 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
     6841 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     6901 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
     6961 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     7021 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     7081 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     7141 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     7201 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     7261 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     7321 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     7381 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     7441 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     7501 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     7561 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     7621 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     7681 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     7741 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     7801 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     7861 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     7921 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     7981 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     8041 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     8101 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     8161 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     8221 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     8281 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     8341 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     8401 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     8461 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     8521 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     8581 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     8641 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     8701 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     8761 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     8821 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     8881 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     8941 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     9001 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     9061 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     9121 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg
     9181 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc
     9241 ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg
     9301 cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga
     9361 tgtacgggcc agatata
//
