LOCUS       sequence-95623-e         457 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak L7Ae-P2A-EYFP 4xFF4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Wroblewska L, Kitada T, Endo K, Siciliano V, Stillo B, Saito H, 
            Weiss R
  TITLE     Mammalian synthetic circuits with RNA binding proteins for RNA-only 
            delivery.
  JOURNAL   Nat Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301.
  PUBMED    26237515
REFERENCE   2  (bases 1 to 457)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301."
COMMENT     Sequence Label: pTK299
FEATURES             Location/Qualifiers
     source          1..457
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(12..68)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 ttgctcacca ttgggcctgg gttctcctcc acgtcgccgg cctgcttcag cagagagaag
       61 ttggtggcgc cagatccctt ctgaaggcct ttaatcttct ccacaaggct tccaagctcc
      121 tttctcagct ctccctcgtt gattatcgct gccgaagcgc atggcacctc aatgcccaca
      181 gcccttccaa ggtcgttctt gcttttaacg taaatgtacg gcacattctt ctcctcgcag
      241 aggaggggca gatgagcaac gatctcaggc gggtcaacat cctctgcgat gtaaacgagc
      301 tttgccagtc ccctctccac agcctttgtc gtctcgttgg tacctttctt taccttaccg
      361 ctctccctaa ccttctccag cagactcaga gcttcgttct gcatgtcctc aggaacctca
      421 aatctcacgt acatggtggc actatgctga ctattta
//
