LOCUS       sequence-95625-e         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP XbaI attB1 Kozak 
            EGFP ochre attB2 AscI (truncated E1) 3'UTR Poly A I-SceI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Wroblewska L, Kitada T, Endo K, Siciliano V, Stillo B, Saito H, 
            Weiss R
  TITLE     Mammalian synthetic circuits with RNA binding proteins for RNA-only 
            delivery.
  JOURNAL   Nat Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301.
  PUBMED    26237515
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301."
COMMENT     Sequence Label: pTK312
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(20..44)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ccttgctcac catggtggca gcctgctttt ttgtacaaac ttgttctaga cttggcggac
       61 tagactatgt cgtagtccat tcaggttagc cgtagagagt tataggggcc cctctcaggt
      121 agctgaatga tttaacactg ctagctagag tagtcatggc cataactatg atggaagttc
      181 ctacggtttc ataccttgat tctactgcct tgcacagctc tgaaagaata cccactcggt
      241 tccagcgtgt tgactcttca tgcaatgccc ttctcctgtc atcatcatgt tcatcgtctg
      301 ctgccagagg tttgccaagc ttaaacagcc tttttagggg gtctgccaca cggcacgctg
      361 tgccggtcac ggagtcacac aaaataaacc ctccacagaa ataaggcgct ttctcgccca
      421 ccacagcatc tataatcttg acttccatat tcaaccaggt ggcgcacctg tctgccatta
      481 atttgtccga tttgactcct ttcacgatat tgtcatctcc aatgaatgct gcacatggtg
      541 atccggttag ccgttctctc aacactctgc ttgcgattac aatgttaatg actgtgttca
      601 caaacagtgt gaggaacatt ccagatttca tcatggctcc gaatttaaat ttagttttag
      661 tgggcaaatg tattgatgaa atttcgccga aagccgcctc aatcagcgtc aacagctctg
      721 cgtccacacc taagtcttcc agaatcatta acgcggtcag agccatggcg tcgtcctcac
      781 ttttatcaa
//
