LOCUS       sequence-9563-ex       10947 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10947)
  AUTHORS   Carey BW, Markoulaki S, Hanna J, Saha K, Gao Q, Mitalipova M, 
            Jaenisch R
  TITLE     Reprogramming of murine and human somatic cells using a single 
            polycistronic vector.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Jan 6. 106(1):157-62.
  PUBMED    19109433
REFERENCE   2  (bases 1 to 10947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2009 Jan 6. 106(1):157-62."
COMMENT     Sequence Label: FUW-SOK
FEATURES             Location/Qualifiers
     source          1..10947
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1686..1919
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2104..2148
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2446..2563
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     2622..2638
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2680..2713)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2763..3974
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3811..3830
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             4990..5055
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             6133..6186
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7660..7719
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     protein_bind    complement(7747..7780)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    7836..8424
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7889..7909)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8307..8318)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8427..8443)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             8953..9133
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(9195..9783)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9275..9294)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9954..10745,10746..10814))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10577..10596
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10815..10919)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg gccgcgctga tcttcagacc tggaggagga
     1561 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     1621 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     1681 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     1741 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     1801 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1861 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1921 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1981 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     2041 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     2101 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     2161 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     2221 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     2281 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     2341 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca
     2401 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg
     2461 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2521 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2581 cagcagagat ccagtttggt tagtaccggg cccgctctag tcgaggtcga cggtatcgat
     2641 aagctcgctt cacgagattc cagcaggtcg agggacctaa taacttcgta tagcatacat
     2701 tatacgaagt tatattaagg gttccaagct taagcggcct aacccgtgtc ggctccagat
     2761 ctggcctccg cgccgggttt tggcgcctcc cgcgggcgcc cccctcctca cggcgagcgc
     2821 tgccacgtca gacgaagggc gcagcgagcg tcctgatcct tccgcccgga cgctcaggac
     2881 agcggcccgc tgctcataag actcggcctt agaaccccag tatcagcaga aggacatttt
     2941 aggacgggac ttgggtgact ctagggcact ggttttcttt ccagagagcg gaacaggcga
     3001 ggaaaagtag tcccttctcg gcgattctgc ggagggatct ccgtggggcg gtgaacgccg
     3061 atgattatat aaggacgcgc cgggtgtggc acagctagtt ccgtcgcagc cgggatttgg
     3121 gtcgcggttc ttgtttgtgg atcgctgtga tcgtcacttg gtgagtagcg ggctgctggg
     3181 ctggccgggg ctttcgtggc cgccgggccg ctcggtggga cggaagcgtg tggagagacc
     3241 gccaagggct gtagtctggg tccgcgagca aggttgccct gaactggggg ttggggggag
     3301 cgcagcaaaa tggcggctgt tcccgagtct tgaatggaag acgcttgtga ggcgggctgt
     3361 gaggtcgttg aaacaaggtg gggggcatgg tgggcggcaa gaacccaagg tcttgaggcc
     3421 ttcgctaatg cgggaaagct cttattcggg tgagatgggc tggggcacca tctggggacc
     3481 ctgacgtgaa gtttgtcact gactggagaa ctcggtttgt cgtctgttgc gggggcggca
     3541 gttatggcgg tgccgttggg cagtgcaccc gtacctttgg gagcgcgcgc cctcgtcgtg
     3601 tcgtgacgtc acccgttctg ttggcttata atgcagggtg gggccacctg ccggtaggtg
     3661 tgcggtaggc ttttctccgt cgcaggacgc agggttcggg cctagggtag gctctcctga
     3721 atcgacaggc gccggacctc tggtgagggg agggataagt gaggcgtcag tttctttggt
     3781 cggttttatg tacctatctt cttaagtagc tgaagctccg gttttgaact atgcgctcgg
     3841 ggttggcgag tgtgttttgt gaagtttttt aggcaccttt tgaaatgtaa tcatttgggt
     3901 caatatgtaa ttttcagtgt tagactagta aattgtccgc taaattctgg ccgtttttgg
     3961 cttttttgtt agacgaagct tgggctgcag gtcgactcta gagaattcgc caccatgtat
     4021 aacatgatgg agacggagct gaagccgccg ggcccgcagc aagcttcggg gggcggcggc
     4081 ggaggaggca acgccacggc ggcggcgacc ggcggcaacc agaagaacag cccggaccgc
     4141 gtcaagaggc ccatgaacgc cttcatggta tggtcccggg ggcagcggcg taagatggcc
     4201 caggagaacc ccaagatgca caactcggag atcagcaagc gcctgggcgc ggagtggaaa
     4261 cttttgtccg agaccgagaa gcggccgttc atcgacgagg ccaagcggct gcgcgctctg
     4321 cacatgaagg agcacccgga ttataaatac cggccgcggc ggaaaaccaa gacgctcatg
     4381 aagaaggata agtacacgct tcccggaggc ttgctggccc ccggcgggaa cagcatggcg
     4441 agcggggttg gggtgggcgc cggcctgggt gcgggcgtga accagcgcat ggacagctac
     4501 gcgcacatga acggctggag caacggcagc tacagcatga tgcaggagca gctgggctac
     4561 ccgcagcacc cgggcctcaa cgctcacggc gcggcacaga tgcaaccgat gcaccgctac
     4621 gacgtcagcg ccctgcagta caactccatg accagctcgc agacctacat gaacggctcg
     4681 cccacctaca gcatgtccta ctcgcagcag ggcacccccg gtatggcgct gggctccatg
     4741 ggctctgtgg tcaagtccga ggccagctcc agcccccccg tggttacctc ttcctcccac
     4801 tccagggcgc cctgccaggc cggggacctc cgggacatga tcagcatgta cctccccggc
     4861 gccgaggtgc cggagcccgc tgcgcccagt agactgcaca tggcccagca ctaccagagc
     4921 ggcccggtgc ccggcacggc cattaacggc acactgcccc tgtcgcacat ggctagcggc
     4981 agcggcccgg tgaaacagac tttgaatttt gaccttctca agttggcggg agacgtcgag
     5041 tccaaccctg ggcccgcatg catggctgga cacctggctt cagacttcgc cttctcaccc
     5101 ccaccaggtg ggggtgatgg gtcagcaggg ctggagccgg gctgggtgga tcctcgaacc
     5161 tggctaagct tccaagggcc tccaggtggg cctggaatcg gaccaggctc agaggtattg
     5221 gggatctccc catgtccgcc cgcatacgag ttctgcggag ggatggcata ctgtggacct
     5281 caggttggac tgggcctagt cccccaagtt ggcgtggaga ctttgcagcc tgagggccag
     5341 gcaggagcac gagtggaaag caactcagag ggaacctcct ctgagccctg tgccgaccgc
     5401 cccaatgccg tgaagttgga gaaggtggaa ccaactcccg aggagtccca ggacatgaaa
     5461 gccctgcaga aggagctaga acagtttgcc aagctgctga agcagaagag gatcaccttg
     5521 gggtacaccc aggccgacgt ggggctcacc ctgggcgttc tctttggaaa ggtgttcagc
     5581 cagaccacca tctgtcgctt cgaggccttg cagctcagcc ttaagaacat gtgtaagctg
     5641 cggcccctgc tggagaagtg ggtggaggaa gccgacaaca atgagaacct tcaggagata
     5701 tgcaaatcgg agaccctggt gcaggcccgg aagagaaagc gaactagcat tgagaaccgt
     5761 gtgaggtgga gtctggagac catgtttctg aagtgcccga agccctccct acagcagatc
     5821 actcacatcg ccaatcagct tgggctagag aaggatgtgg ttcgagtatg gttctgtaac
     5881 cggcgccaga agggcaaaag atcaagtatt gagtattccc aacgagaaga gtatgaggct
     5941 acagggacac ctttcccagg gggggctgta tcctttcctc tgcccccagg tccccacttt
     6001 ggcaccccag gctatggaag cccccacttc accacactct actcagtccc ttttcctgag
     6061 ggcgaggcct ttccctctgt tcccgtcact gctctgggct ctcccatgca ttcaaacgca
     6121 tgcggctccg gcgagggcag gggaagtctt ctaacatgcg gggacgtgga ggaaaatccc
     6181 ggcccactcg agatgaggca gccacctggc gagtctgaca tggctgtcag cgacgctctg
     6241 ctcccgtcct tctccacgtt cgcgtccggc ccggcgggaa gggagaagac actgcgtcca
     6301 gcaggtgccc cgactaaccg ttggcgtgag gaactctctc acatgaagcg acttccccca
     6361 cttcccggcc gcccctacga cctggcggcg acggtggcca cagacctgga gagtggcgga
     6421 gctggtgcag cttgcagcag taacaacccg gccctcctag cccggaggga gaccgaggag
     6481 ttcaacgacc tcctggacct agactttatc ctttccaact cgctaaccca ccaggaatcg
     6541 gtggccgcca ccgtgaccac ctcggcgtca gcttcatcct cgtcttcccc agcgagcagc
     6601 ggccctgcca gcgcgccctc cacctgcagc ttcagctatc cgatccgggc cgggggtgac
     6661 ccgggcgtgg ctgccagcaa cacaggtgga gggctcctct acagccgaga atctgcgcca
     6721 cctcccacgg cccccttcaa cctggcggac atcaatgacg tgagcccctc gggcggcttc
     6781 gtggctgagc tcctgcggcc ggagttggac ccagtataca ttccgccaca gcagcctcag
     6841 ccgccaggtg gcgggctgat gggcaagttt gtgctgaagg cgtctctgac cacccctggc
     6901 agcgagtaca gcagcccttc ggtcatcagt gttagcaaag gaagcccaga cggcagccac
     6961 cccgtggtag tggcgcccta cagcggtggc ccgccgcgca tgtgccccaa gattaagcaa
     7021 gaggcggtcc cgtcctgcac ggtcagccgg tccctagagg cccatttgag cgctggaccc
     7081 cagctcagca acggccaccg gcccaacaca cacgacttcc ccctggggcg gcagctcccc
     7141 accaggacta cccctacact gagtcccgag gaactgctga acagcaggga ctgtcaccct
     7201 ggcctgcctc ttcccccagg attccatccc catccggggc ccaactaccc tcctttcctg
     7261 ccagaccaga tgcagtcaca agtcccctct ctccattatc aagagctcat gccaccgggt
     7321 tcctgcctgc cagaggagcc caagccaaag aggggaagaa ggtcgtggcc ccggaaaaga
     7381 acagccaccc acacttgtga ctatgcaggc tgtggcaaaa cctataccaa gagttctcat
     7441 ctcaaggcac acctgcgaac tcacacaggc gagaaacctt accactgtga ctgggacggc
     7501 tgtgggtgga aattcgcccg ctccgatgaa ctgaccaggc actaccgcaa acacacaggg
     7561 caccggccct ttcagtgcca gaagtgcgac agggcctttt ccaggtcgga ccaccttgcc
     7621 ttacacatga agaggcactt ttaactcgag ggctcgggcc agtgtactaa ttatgctctc
     7681 ttgaaattgg ctggagatgt tgagagcaac ccaggtcccg gcgcgccgaa ttcgtcgagg
     7741 gacctaataa cttcgtatag catacattat acgaagttat acatgtttaa gggttccggt
     7801 tccactaggt acaattcgat atcaagctta tcgataatca acctctggat tacaaaattt
     7861 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     7921 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     7981 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     8041 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     8101 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     8161 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     8221 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     8281 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     8341 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     8401 tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgatcgaga cctagaaaaa
     8461 catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa
     8521 gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg
     8581 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     8641 ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc
     8701 tacttccctg attggcagaa ctacacacca gggccaggga tcagatatcc actgaccttt
     8761 ggatggtgct acaagctagt accagttgag caagagaagg tagaagaagc caatgaagga
     8821 gagaacaccc gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa
     8881 gtattagagt ggaggtttga cagccgccta gcatttcatc acatggcccg agagctgcat
     8941 ccggactgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     9001 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     9061 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     9121 aaaatctcta gcagcatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     9181 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     9241 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     9301 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     9361 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     9421 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     9481 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     9541 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     9601 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     9661 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     9721 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     9781 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     9841 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     9901 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     9961 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
    10021 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
    10081 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
    10141 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
    10201 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
    10261 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
    10321 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
    10381 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
    10441 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
    10501 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
    10561 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
    10621 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
    10681 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
    10741 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
    10801 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
    10861 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
    10921 acatttcccc gaaaagtgcc acctgac
//
