LOCUS       sequence-95639-e        1052 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  retroviral pBabe puro vector encoding N-terminally FLAG tagged 
            mutant human IRE1a deleted from amino acids P28 to D408 and bearing 
            mutation K599A (kinase domain mutant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Volmer R, van der Ploeg K, Ron D
  TITLE     Membrane lipid saturation activates endoplasmic reticulum unfolded 
            protein response transducers through their transmembrane domains.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Mar 19;110(12):4628-33. doi: 
            10.1073/pnas.1217611110. Epub 2013 Mar 4.
  PUBMED    23487760
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1217611110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-03-19- 19"; volume: "110"; issue: "12"; pages: "4628-33"
COMMENT     Sequence Label: Flag_HsIRE1a_deltaP29_D408_K599A_pBabePuro
FEATURES             Location/Qualifiers
     source          1..1052
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..22
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             42..86
                     /codon_start=1
                     /product="leader sequence from mouse preprotrypsin"
                     /label=preprotrypsin leader
                     /translation="MSALLILALVGAAVA"
     CDS             87..110
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cctcccttta tccagccctc actccttctc taggcgccgg catgtctgca cttctgatcc
       61 tagctcttgt tggagctgca gttgctgact acaaagacga tgacgacaag ctgatttttg
      121 gaagtaccag cacagtgacg cttcagactt cagaaaacgc acctaccacc gtgtctcggg
      181 atgtggagga gaagcccgcc catgcccctg cccggcccga ggcccccgtg gactccatgc
      241 ttaaggacat ggctaccatc atcctgagca ccttcctgct gattggctgg gtggccttca
      301 tcatcaccta tcccctgagc atgcatcagc agcagcagct ccagcaccag cagttccaga
      361 aggaactgga gaagatccag ctcctgcagc agcagcagca gcagctgccc ttccacccac
      421 ctggagacac ggctcaggac ggcgagctcc tggacacgtc tggcccgtac tcagagagct
      481 cgggcaccag cagccccagc acgtccccca gggcctccaa ccactcgctc tgctccggca
      541 gctctgcctc caaggctggc agcagcccct ccctggaaca agacgatgga gatgaggaaa
      601 ccagcgtggt gatagttggg aaaatttcct tctgtcccaa ggatgtcctg ggccatggag
      661 ctgagggcac aattgtgtac cggggcatgt ttgacaaccg cgacgtggcc gtggcgagga
      721 tcctccccga gtgttttagc ttcgcagacc gtgaggtcca gctgttgcga gaatcggatg
      781 agcacccgaa cgtgatccgc tacttctgca cggagaagga ccggcaattc cagtacattg
      841 ccatcgagct gtgtgcagcc accctgcaag agtatgtgga gcagaaggac tttgcgcatc
      901 tcggcctgga gcccatcacc ttgctgcagc agaccacctc gggcctggcc cacctccact
      961 ccctcaacat cgttcacaga gacctaaagc cacacaacat cctcatatcc atgcccaatg
     1021 cacacggcaa gatcaaggcc atgatctccg ac
//
