LOCUS       sequence-95641-e        1049 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-terminally FLAG tagged human IRE1a in retroviral pBabe puro 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Volmer R, van der Ploeg K, Ron D
  TITLE     Membrane lipid saturation activates endoplasmic reticulum unfolded 
            protein response transducers through their transmembrane domains.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Mar 19;110(12):4628-33. doi: 
            10.1073/pnas.1217611110. Epub 2013 Mar 4.
  PUBMED    23487760
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1217611110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-03-19- 19"; volume: "110"; issue: "12"; pages: "4628-33"
COMMENT     Sequence Label: Flag_HsIRE1a_pBabePuro
FEATURES             Location/Qualifiers
     source          1..1049
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..23
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             43..87
                     /codon_start=1
                     /product="leader sequence from mouse preprotrypsin"
                     /label=preprotrypsin leader
                     /translation="MSALLILALVGAAVA"
     CDS             88..111
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tcctcccttt atccagccct cactccttct ctaggcgccg gcatgtctgc acttctgatc
       61 ctagctcttg ttggagctgc agttgctgac tacaaagacg atgacgacaa gctgattttt
      121 ggaagtacca gcacagtgac gcttcctgaa accttgttgt ttgtgtcaac gctggatgga
      181 agtttgcatg ctgtcagcaa gaggacaggc tcaatcaaat ggactttaaa agaagatcca
      241 gtcctgcagg tcccaacaca tgtggaagag cctgcctttc tcccagatcc taatgatggc
      301 agcctgtata cgcttggaag caagaataat gaaggcctga cgaaacttcc ttttaccatc
      361 ccagaattgg tgcaggcatc cccatgccga agttcagatg gaatcctcta catgggtaaa
      421 aagcaggaca tctggtatgt tattgacctc ctgaccggag agaagcagca gactttgtca
      481 tcggcctttg cagatagtct ctgcccatca acctctcttc tgtatcttgg gcgaacagaa
      541 tacaccatca ccatgtacga caccaaaacc cgagagctcc ggtggaatgc cacctacttt
      601 gactatgcgg cctcactgcc tgaggacgac gtggactaca agatgtccca ctttgtgtcc
      661 aatggtgatg ggctggtggt gactgtggac agtgaatctg gggacgtcct gtggatccaa
      721 aactacgcct cccctgtggt ggccttttat gtctggcagc gggagggtct gaggaaggtg
      781 atgcacatca atgtcgctgt ggagaccctg cgctatctga ccttcatgtc tggggaggtg
      841 gggcgcatca caaagtggaa gtacccgttc cccaaggaga cagaggccaa gagcaagctg
      901 acgcccactc tgtatgttgg gaaatactct accagcctct atgcctctcc ctcaatggta
      961 cacgaggggg ttgctgtcgt gccccgcggc agcacacttc ctttgctgga agggccccag
     1021 actgatggcg tcaccatcgg ggacaaggg
//
