LOCUS       sequence-95643-e        1069 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  retroviral pBabe puro vector encoding N-terminally FLAG tagged 
            mutant human IRE1a deleted from amino acids P28 to D408.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Volmer R, van der Ploeg K, Ron D
  TITLE     Membrane lipid saturation activates endoplasmic reticulum unfolded 
            protein response transducers through their transmembrane domains.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Mar 19;110(12):4628-33. doi: 
            10.1073/pnas.1217611110. Epub 2013 Mar 4.
  PUBMED    23487760
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1217611110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-03-19- 19"; volume: "110"; issue: "12"; pages: "4628-33"
COMMENT     Sequence Label: Flag_HsIRE1a_deltaP29_D408_pBabePuro
FEATURES             Location/Qualifiers
     source          1..1069
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..23
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             43..87
                     /codon_start=1
                     /product="leader sequence from mouse preprotrypsin"
                     /label=preprotrypsin leader
                     /translation="MSALLILALVGAAVA"
     CDS             88..111
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tcctcccttt atccagccct cactccttct ctaggcgccg gcatgtctgc acttctgatc
       61 ctagctcttg ttggagctgc agttgctgac tacaaagacg atgacgacaa gctgattttt
      121 ggaagtacca gcacagtgac gcttcagact tcagaaaacg cacctaccac cgtgtctcgg
      181 gatgtggagg agaagcccgc ccatgcccct gcccggcccg aggcccccgt ggactccatg
      241 cttaaggaca tggctaccat catcctgagc accttcctgc tgattggctg ggtggccttc
      301 atcatcacct atcccctgag catgcatcag cagcagcagc tccagcacca gcagttccag
      361 aaggaactgg agaagatcca gctcctgcag cagcagcagc agcagctgcc cttccaccca
      421 cctggagaca cggctcagga cggcgagctc ctggacacgt ctggcccgta ctcagagagc
      481 tcgggcacca gcagccccag cacgtccccc agggcctcca accactcgct ctgctccggc
      541 agctctgcct ccaaggctgg cagcagcccc tccctggaac aagacgatgg agatgaggaa
      601 accagcgtgg tgatagttgg gaaaatttcc ttctgtccca aggatgtcct gggccatgga
      661 gctgagggca caattgtgta ccggggcatg tttgacaacc gcgacgtggc cgtgaagagg
      721 atcctccccg agtgttttag cttcgcagac cgtgaggtcc agctgttgcg agaatcggat
      781 gagcacccga acgtgatccg ctacttctgc acggagaagg accggcaatt ccagtacatt
      841 gccatcgagc tgtgtgcagc caccctgcaa gagtatgtgg agcagaagga ctttgcgcat
      901 ctcggcctgg agcccatcac cttgctgcag cagaccacct cgggcctggc ccacctccac
      961 tccctcaaca tcgttcacag agacctaaag ccacacaaca tcctcatatc catgcccaat
     1021 gcacacggca agatcaaggc catgatctcc gactttggcc tctgcaaga
//
